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Pigx (NM_024464) Mouse Tagged ORF Clone
CAT＃: MG201059
- TrueORF®
Pigx (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx)
ORF Plasmid: DDK
"NM_024464" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2010319C14Rik; PIG-X |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201059 representing NM_024464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTATCAGAAAGTTTTAATATAGAAGCCCCCAACTACTTGTCCAATGAATCTGCAGTCCTCATTTATG CCCGGCAGGATGCACAGTGCATCGACTGCTTCCAGGCCTTTTTACCTGTGCACTATCGCTATCACCGGCC ACATAAGAAAGATGGAGACACCCTCATTGTGGTCAACAACCCCGACTTACTGATGTACTGTGACCAAGAG TTTCCAATTTTGAAATGCTGGGCTCAGTCAGAAGTAGCAGCTCCTTGTGCTTTGAAGAGTGAGGAGATCT GCCAGTGGAAAAGCATGCAGTACAAATCAATACTTAAGAATTTGACAGTGCAGGTTCCAGTGGGACTGAC TATACATACCTCTTTAGTGTGTTCTGTGACGCTGCTCATTACGATTCTGTGTTCTACTTTGATCCTTTTA GCTGTTTTCAAATATGGCCACTTTTCCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201059 representing NM_024464
Red=Cloning site Green=Tags(s) MLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLIVVNNPDLLMYCDQE FPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVPVGLTIHTSLVCSVTLLITILCSTLILL AVFKYGHFSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024464 |
| ORF Size | 450 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024464.2, NP_077784.1 |
| RefSeq Size | 984 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 72084 |
| UniProt ID | Q99LV7 |
| Gene Summary | This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200047 | Pigx (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 1, (10ug) |
CNY 1,200.00 |
|
| MR201059 | Pigx (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 1 |
CNY 1,200.00 |
|
| MR201059L3 | Lenti ORF clone of Pigx (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 1 |
CNY 4,750.00 |
|
| MR201059L4 | Lenti ORF clone of Pigx (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 1 |
CNY 4,750.00 |
