Mthfs (BC024567) Mouse Tagged ORF Clone
CAT#: MG200973
- TrueORF®
Mthfs (tGFP-tagged) - Mouse 5, 10-methenyltetrahydrofolate synthetase (cDNA clone MGC:37660 IMAGE:5026828)
ORF Plasmid: DDK
"BC024567" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Gm2382 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200973 representing BC024567
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGATGAAGTTGAGACGGAAGTAATCATCAAGGACATTTTCAAACAAGGCAAAATCTGCTTCATCC CTCAGTACCAATTCCAGAGCAATCACATGGACATGGTGAGGTTAACGTCACCTGAAGAAATTGCTTTACT TCCCAAGACATCTTGGAATATTCATCAGCCTGGTGAGGGAGATGTTCGGGAGGAGGCCTTATCCACTGGT GGACTTGACCTCATCTTCCTTCCTGGCCTCGGGTTTGACAAAGATGGCAACCGGCTGGGGCGGGGTAAGG GGTACTATGACACCTACCTGAAGCGCTGTGTACAGCATCAGGAAGTGAAGCCCTACACCATGGCTTTGGC TTTTAAAGAGCAGATCTGTCCCCAGATCCCAGTGGATGAGCATGACATGAAGGTAGATGAAGTCCTTTAT GAAGATTCCCCAGCATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200973 representing BC024567
Red=Cloning site Green=Tags(s) MQDEVETEVIIKDIFKQGKICFIPQYQFQSNHMDMVRLTSPEEIALLPKTSWNIHQPGEGDVREEALSTG GLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEVKPYTMALAFKEQICPQIPVDEHDMKVDEVLY EDSPAS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC024567 |
ORF Size | 440 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC024567, AAH24567 |
RefSeq Size | 689 bp |
RefSeq ORF | 440 bp |
Locus ID | 107885 |
Gene Summary | Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-CHO-H(4)PteGlu) to yield 5,10-methenyltetrahydrofolate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC206973 | Mthfs (untagged) - Mouse 5, 10-methenyltetrahydrofolate synthetase (cDNA clone MGC:37660 IMAGE:5026828), (10ug) |
CNY 3,230.00 |
|
MR200973 | Mthfs (Myc-DDK-tagged) - Mouse 5, 10-methenyltetrahydrofolate synthetase (cDNA clone MGC:37660 IMAGE:5026828) |
CNY 1,200.00 |
|
MR200973L3 | Lenti ORF clone of Mthfs (Myc-DDK-tagged) - Mouse 5, 10-methenyltetrahydrofolate synthetase (cDNA clone MGC:37660 IMAGE:5026828) |
CNY 4,750.00 |
|
MR200973L4 | Lenti ORF clone of Mthfs (mGFP-tagged) - Mouse 5, 10-methenyltetrahydrofolate synthetase (cDNA clone MGC:37660 IMAGE:5026828) |
CNY 4,750.00 |