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Brms1 (BC003485) Mouse Tagged ORF Clone
CAT＃: MG200909
- TrueORF®
Brms1 (tGFP-tagged) - Mouse breast cancer metastasis-suppressor 1 (cDNA clone MGC:6927 IMAGE:2811430)
ORF Plasmid: DDK
"BC003485" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AV003220; AW554636 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG200909 representing BC003485
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCCAGCCTTCAGGCAAAGAAACAGAAGAGATGGAGGCAGAAGGGGATTCAGCAGCTGAGATGA ACGGGGAGGCAGATGAGAGCGAGGAGGAGCGGAGTGGCAGCCAAACTGAGTCAGAGGAGGAGAGCTCAGA GATGGATGATGAAGACTATGAGCGGCGCCGCAGTGAGTGTGTCAGTGAGATGCTGGACCTGGAAAAGCAG TTCTCAGAGCTGAAGGAGAAGTTGTTCAGGGAACGGCTCAGCCAGCTACGTTTACGACTGGAGGAAGTGG GGGCTGAGAGAGCCCCAGAATATACGGAACCTCTTGGCGGGCTGCAGCAGAGCCTGAAGATCCGCATTCA GGTGGCAGGGATCTACAAGGGCTTCTGTCTGGATGTGATCAGGAATAAGTATGAGTGTGAACTCCAGGGA GGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200909 representing BC003485
Red=Cloning site Green=Tags(s) MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQ FSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQG G TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC003485 |
| ORF Size | 425 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003485, AAH03485 |
| RefSeq Size | 1212 bp |
| RefSeq ORF | 425 bp |
| Locus ID | 107392 |
| Gene Summary | Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR200909 | Brms1 (Myc-DDK-tagged) - Mouse breast cancer metastasis-suppressor 1 (cDNA clone MGC:6927 IMAGE:2811430) |
CNY 1,200.00 |
|
| MR200909L3 | Lenti ORF clone of Brms1 (Myc-DDK-tagged) - Mouse breast cancer metastasis-suppressor 1 (cDNA clone MGC:6927 IMAGE:2811430) |
CNY 4,750.00 |
|
| MR200909L4 | Lenti ORF clone of Brms1 (mGFP-tagged) - Mouse breast cancer metastasis-suppressor 1 (cDNA clone MGC:6927 IMAGE:2811430) |
CNY 4,750.00 |
