Lsm2 (BC049543) Mouse Tagged ORF Clone
CAT#: MG200745
- TrueORF®
Lsm2 (tGFP-tagged) - Mouse LSM2 homolog, U6 small nuclear RNA associated (S
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CNY 2,090.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Sm-X5, G7b, SmX5, snRNP, MGC13889 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200745 representing BC049543
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTTGGGGGAAACCTTTAGCAAGTCCAAGAGAAAGCCCACGGGCGCCGGGGGAGAACGGGAAGC GAGCCTTGCACTTCTGTGCTGTTGACGAGGACTTTGTGAGGCTCTTCTACTCCTTTTTCAAGTCTCTTGT GGGCAAGGATGTAGTCGTGGAACTCAAGAATGACCTGAGCATCTGTGGAACCCTCCACTCTGTGGACCAG TACCTCAATATCAAACTAACCGACATCAGTGTCACAGACCCTGAGAAATACCCTCACATGTTATCAGTCA AGAACTGCTTCATCAGGGGCTCAGTCGTCCGCTATGTGCAGCTGCCAGCAGATGAGGTGGACACACAACT GCTACAGGATGCAGCAAGGAAGGAAGCCCTTCAGCAGAAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200745 representing BC049543
Red=Cloning site Green=Tags(s) MVFWGKPLASPRESPRAPGENGKRALHFCAVDEDFVRLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQ YLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEALQQKQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC049543 |
ORF Size | 395 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC049543, AAH49543 |
RefSeq Size | 1090 bp |
RefSeq ORF | 395 bp |
Locus ID | 27756 |
Gene Summary | Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.[UniProtKB/Swiss-Prot Function] |
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