Eif4ebp3 (NM_201256) Mouse Tagged ORF Clone
CAT#: MG200316
- TrueORF®
Eif4ebp3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E binding protein 3 (Eif4ebp3)
ORF Plasmid: DDK
"NM_201256" in other vectors (4)
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CNY 2,800.00
CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4e-bp3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200316 representing NM_201256
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGTCCACGAGTTGCCCAATTCCAGGGTGCCGGGACCAGCTGCCAGACGGCTACAGCACCACGC CTGGCGGCACACTATACGCCACTACCCCGGGAGGCACCAGGATCATCTACGACAGAAAGTTCCTGCTGGA GTGCAAGAACTCACCCATTGCCCGGACACCTCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCTC CCAGCAGTACCACCCTCCAAGTTGGAGTTGCTGAAGGAACAGAAGCAGACAGAGGTAGAAATAACCGATG ACGAACAGTTTGAAATGGACATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200316 representing NM_201256
Red=Cloning site Green=Tags(s) MSSSTSCPIPGCRDQLPDGYSTTPGGTLYATTPGGTRIIYDRKFLLECKNSPIARTPPCCLPQIPGVTTL PAVPPSKLELLKEQKQTEVEITDDEQFEMDM TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_201256 |
ORF Size | 303 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201256.4, NP_957708.1 |
RefSeq Size | 1151 bp |
RefSeq ORF | 306 bp |
Locus ID | 108112 |
UniProt ID | Q80VV3 |
Gene Summary | Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204980 | Eif4ebp3 (untagged) - Mouse eukaryotic translation initiation factor 4E binding protein 3 (Eif4ebp3), (10ug) |
CNY 1,200.00 |
|
MR200316 | Eif4ebp3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E binding protein 3 (Eif4ebp3) |
CNY 1,200.00 |
|
MR200316L3 | Lenti ORF clone of Eif4ebp3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E binding protein 3 (Eif4ebp3) |
CNY 4,750.00 |
|
MR200316L4 | Lenti ORF clone of Eif4ebp3 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4E binding protein 3 (Eif4ebp3) |
CNY 4,750.00 |
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