Muc4 (NM_080457) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933405I11Rik; Asgp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229775 representing NM_080457 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGGGCCTCACTGGAGAGTTCCCTGGCTGTGTCTGAGCTGCCTGTATTCTTGCCTCCTCTTGCTAC CTGATGCTCTAGCAACAACATCAACACAGACACCCATGTCCCTTTCCAGCAGCACCAGAACATCCCAAAT GAGCTCACAAGCATCGACCTCATCAACATCTTCTGACAGAAGGACAAGTAAAACAGAACAAACATCAACA AGGGATACTCCAAGTTCCATAACAACAGTTTCTCAGAGCCATCATACTACAAGCATGGAGACCAGCAAAC CTCAAACCACCACAACCACCGAGGTGACCACATCAACTCCTTCAGCCTCCTCACGTGACCAAATACAGAC AGAGACAAGTTCTCAAAGAACAATCTCTCCTGATGGAACAACCACCTCACATGCTCCCAGTATCAGCAGC TCAGCTCCAAGTACAACACACATGTTAACCACAACATCCTCCACAGAAAGTACCTCAGTAGACTCAGGAC ACACAACAGCAATAACAACTCAAGGTTTAACACCTGCCACCGCACAAGTCTCACTGACACCTTCATCCCA GAATATGTCAACAGTGTCAACACCCATCACCTCAACTCTTACTCAGAGACAACACACTGGAAGCAAGCAG ACCAGCAGCAAATCTCAAGTCAACATAGTCACATCAACTCTTTCAACCTCTACAAGTGACTCAACTCCTG CACAGACAATGTCTCAAGTGACATCATCTTCAGATAAAAGAACCAAGCCATCTACTTCTGGTGTCAGCAG TACATCACTAACTACAACAGAAGTGCTCACCCAAACATCCTCTACAGACTCTGCTCCAGGAAACACAACT CTGAGGATTACTCAGAACTCTACAACACATACCACGAAAGTCTCAACAACATCTACACCACAAAAACTGT CACCAGTGTCAACACTCATCAATTCAAGTCAAAAAATGTCAACCCTTCCTCAGAACCAACACACTGAAAG CATGGACACCAGCAGACAACCTCAAACCACCACAACCATAGAGGTGACCACATCAACCCCTTCAGCCTCC TCACTTCACCAAATTCAGACAGAGACAAATTCCCCAAAAACAATCTCTCCTGGTGAGACAACCACCTCAC ATGCTCCGAACATGAGAAGCTCACCACCAAAGACATCACAGATACTAACCACAATGCCCTCCACAAAAAG TACCTCAGTAGACACAAAACAAACAAAGGCAATAACTACTAAAGTTTCTACACCTGACACCACACAGGTC TCGATGACACCTTCATCCCAGAAATTGCCAACACACAGCACGTCTACTCAGGAATTAACATCTTCTTACA GCCAACATATTCAAAGCAAGGGGACCAGCAGCAAATCTCAAACAACCACAAACACCAAGGTGAACACATC AACTCCTTCAGCCTCCTCACGTGACAAAATTCAGACAGAGACAAGTTCCCAAAGAACAAACTCTCCTGGC GAGAAAAGGACATCACATGCTCCCAGCATGAGCAGCTCAGCTCCAAGTACAACACACATGTTATCCACAA CATCCTCCAATCAAAGTACCTCAGTAGACACAGGACAAACAACATCAGTAACAGCTCAAGGTTCTACACC TGCCATCACACAGACCTCATTGACACCTTCATCCCAGAATACGTCAACAGTGTCAACACCCATCACCTCT ACTCACAAATTGTCAACCCTTTCTCAGAGCCAACACACTGGAAGCAAGGGGACCAGCAGCAATCCTCAAA CTACCACCACCACAGAGGTGACCACATCAACTCCTTCAGCCACCACCCATGACCAAATTCAGACAGAGAC AAGTTCCCAAAACACAATCTCTCCTGGTGAGACAACCACCTCATATGCTCCCATCATGAGCAGCTCAGCT CCAAGTACAACACATATGTTATCCACAACATCCTCCACTCAAAGTACCTCAGTAGACACAAGACACACTA CAACATTAACGAATCAAGGATCTACACCTGCCACCACACAGGTCTCACCTTCATCCCAGAACATGTCAAC AGTGTCAGCACCCATCACCTCAACTCAAATTTTGTCAACCTTTCCTCAGAGCCAACACACTGGAAGCAAG GGGACCAGCAGCAATCCTCAAACTACCACCACCCCAGTGGTGACCACATCAAATCCTTCAGCCACCTCAC GTGACCAAATTCAGACAGAGACAAGTTCCCAAAGAACAATCTCTCCTGGTGAGACAACCACCTCATATGC TTCCATCATGAGCAGCTCAGCTCCAAGTACAACACACATGTTAACCACAACATCCTCCACTCAAAGTACC TCAGTAGACACAAGACACACAATAGCAGTAAGGACTCAAGGTTCTACACCTGCCACCACACAGGTCTCAC CTTCATCCCAGAACATGTCAACAGTGTCAACACCCATCACCTCAACTCAAATTTTGTCAACCCTTCCTCA GAGCCAACACACTGGAAGCAAGGGGACCAGCAGCAATCCTCAAACTACCACAAGCCCAGTGGTAACCACA TCAACTCCTTCAGGCACCTCAGGTGACCAAATTCAGACAGAGACAAGTTCCCAAAGAACAATCTCTCCCG GCAAGACAACCACTTCACATGCTCTCAACATAAACAGCTCAGCTCCAAGTACAACACACATGTTATCCAC AACATCCTCCACTCAAAGTACCTCAGGAGACACAAGACACACAACAGCAGGAAGGACTCAAGGTTCTACA CCTGCCACCACACAGGTCTCACCTTCATCCCAGAACATGTCAACAGTGTCAGCACCCATCACCTCAATTC AAATGTTGTCAACCCTTCCTCAGAGCCAACACACTGAAAGCAAGAGTACCAGCACCAATCCTCAAACTAC CACCACCCCAGAGGTGACCACATCAAATCCTTCAGCCACCTCACATGACCAAATTGAGACAGAAACAAGT TCCCAAAGAACAATCTCTCCTGGTGAGACAACCACCTCATATGCTCCCATCATGAGCAGCTCAGCTCCAA GTACAACACACATGTTATCCACAACATCCTCCACTCAAAGTACCTCAGTAGACACAAGAAACACTACAAC ATTAACGACTCAAGGATCTACACCTGCCACCACACAGGTCTCACCTTCATCCAAGAACATGTCAACAGTG TCAACACCAATCACCTCTACTCACAAATTGTCAACCCTTCCTCAGAGCCAACACACTGGAAGCAATGGGA CCAGCAGCTCAAGTTCTACACCTGCCACACACAGGTCTCACCTTCATCCCAACATGTCAACAGTGTCAAC ACCCATCACCACTACTCACAAATTGTCAACCCTTTCTCAGAGCCAACACACTGGAAGCAAGGGGACCAGC AGCAATCCTCAAACTACCACAACGCCAGTGATGACCACATCAACTCCTTCAGCCACCACCCATGACCAAA TTCAGACAGAGACAAGTTCTCAAAGAACAATCTCTCATGGTGAGACAACCACCTCATATGCTCCCATCAT GAGCAGCTCAGCTCCAAGTACAACACACATGTTATCAACAGCATCCTCTACTCAAATTACCTCAGTAGAC ACAAGACACACAACAGCAATAACAACTCAAGGTTCTACACCTGCCACCACACAGGTCTCACCTTCATCCC AGAACATGTCAACAGTGTCAGCACCCATCACCTCAATTCAAATTTTGTCAACCCTTCCTCAGAGCCAACA CACTGGAAGCAAGGGGACCAGCACCAATCCTCAAACTACCACCACCCCAGAGGTGACCACATCAACTCCT TCAGCCACCTCACGTGACCAAATTCAGACAGAGACAAGTTCCCAAAGAACAATCTCTCCTGGTGAGACAA CCACCTCACATGCTCCCATCATGAGCAGCTCAGCTCCAAGTACAACACACATGTTATCCACAACATCCTC CACTCAAAGTACCTCAGTAGACACAAGACACACAACAGCAGGAAGGACTCAAGGTTCTACACCTGCCACC ACACAGGTCTCACCTTCCTCCCAGAACATGACAACCACCTCACATGCCCTCATGAGCAGCTCAGCTCCAA GTACAACACACATGTTATCCACAACATCTTCCACTCAAAGTACCTCAGTAGATACAAGACACACTACAAC AGTAACGACTCAAGGTTCTACACCTGCCACCACACAGGTCTTACCTTCATCCCAGAACATGTCAACAGTG TCAGCTCCCATCACCTCAACTCAAATTTTGTCAACCCTTCCTCAGAGCCAACACACTGGAAGCAAGGGGA CCAGCACCAATCCTCAAACTACCACCAATGCAGAGGTGACCACATCAACTCCTTCAGCCACCTCACATGA CCAAATTGAGACAGAAACAAGTTCCCAAAGAACAATCTCTCCTGGTGAGACAACCACCTCACATGCTCCC AACATGAGTAGTTCAGCTCCAAGTACAACACACATGTTATCCTCAACATCCTCCACTCAAATTACCTCAG TAGACACAGGACACACATCAGCAGGAAGGACTCAAGGTTCTACACCTGCCACCACACAGGTCTCACCTTC ATCCCAGAACATGTCAACAGTGTCAGCACCCATCACCTCAACTCATATTTTGTCAACCCTTCCAAAGAGC CAACACACTGGAAGCAAGGGGACCAGCAGCAATCCTCAAACTACCATAACCCCAGTGGTAACCACATCAA CTCCTTCAGCCTCCTCCCGTGACCAAATTCAGACAGAGACTAGTTTCCAAAGAACAATCTCTCCTGGCGA GACAACCACCTCACATGCTCCCAGCATGAGCAGCTCAGCTCCAAGTTCAACACACATGTTATCAACAGCA TCCTCTACTCAAATTACCTCAGTAGACACAAGACACACAACAGCAATAACAACTCAAGGTTCTACACCTG CCACCACACAGGTCTCACCTTCATCCCAGAACATGTCAACAGTGTCAGCACCCATCACCTCAATTCAAAT GTTGTCAACCCTTCCTCAGAGCCAACACACTGAAAGCAAGAGTACCAGCACCAATCCTCAAACTACCACC ACCCCCAGAGTGACCACATCAACTCCTTCAGCCTCCTCACGTGACCAAATTCAGACAGAGACAAGTTCCC AAAGAACAATCTCTCCCGGCAAGACAACCACCTCACATGTTCCCAACATGAACAGCTCAGCTCCAAGTAC AACACACATCTTATCCACAACATCCTCCATTCAAAGTACCTCAGGAGATACAAGACACACAACAGCAGTA AGGACTCAAGGTTCTACACCTGCCACCACACAGGTCTCACTGGCACCTTCATCCCAGAACATGTCAACAC TGTCAGCACCCATCACATCACCTCAACATTTTTCAACCCTTCCTCAGAACCAACACACTGGAAGCATGGG GACCAGCAGCAATCCTCAAAGTACCACCATCCCAGAGGTGACCACATCAACTCTTTCAGCTTCCTCACGT GACCAAGTTCAGACAGAGACAAGTTCCCAAAGAACAATCTCTCCTGGTGAGACAACCACCTCACATGCTT CCAGCTTGAGCAGTTCAGCTCCAAGTTCAACACACATGTTATCCACAGCATCCTCCACTGAAATTACCTC AGGAGACACAAGACACACAACAGCAATAGTGACTCAAGGTTCTACACCTGCCACCACACAGACCACATTG ACACCTTCGTCCCAGAATATGTCAACAGTGTCAACACCCATCACTTCTACTCACAAATTGTCACCCCTTC CTCAGAGCCAACACACTGAAAACATGGGGACCAGCAGCAATCCTCAAACTACCACCACCCCAGAAGTGAC CACATCAACTCCTTCAGCCACCTCCTATGACCAAATTCAGACAGAGACAAGTTTCCAAAGAACAATCTCT CCTGGTGAGACAACCACTTCACATGCTCCCAGCATGAGCAACTCAGCTCCAAGTTCAACACACAAGTTAT CAACAGCATCCTCCACTGAAATTACCTCAGTAGACACAAGACACACAATAGCAATAACGACTCAAGGTTC TACACTTGCCACCACACAGACCTCACTGACACCTTCATCCCAGAACATGTCAACAGTGTCAGCACCCATC ACCTCATCTCAAATTTTGTCAACTCTTCGTCAGAGCCAACACACTGGAAGCAAGGGGACCAGCAGCAATC ATCAGACTACCACTACCCCTGTGGTGACCACATCAACTTCTTCAGCCACCTCACGTGACCAAATTCAGAC AGAGACAAGTTCCCTAAGAACAATCTCTCCTGATGGAACGACCACCTCACATGCTTCCAGCATGAGCAGT TCATCTCCAAATACAACACACTTGTTAATCACTACATCCTCCACAGAAAGTACCTCAGTAGACACAGGAT ACTCAACAGTAATAACGACTCATGGTTCTACACTTGCCACCACCCAGGTCTCATTGACACCTTCATCCCA GAATATGTCAACAGTGTCAATGCCCAGCACTTCTTCACAGGAATTAACATCACTTCCTCAGAGACAACAC ACTGGAAACATGGAGACCAGCAGCCAACCTCAGAATATCACCCCTACTGTGGTGACCACATCAACTCTTT TATCATTCTCAAGGGGATCCACTGAATTGCAAACCATGTCTTGGGGAACGTCCTCCTCAGGGACAATAAA TACCCTATCTACTCCCGTCAGGAATACCTCACCTGCATCCACATCAGGCATCCTCACCAGCACATTGACC ACCTCAGGAAACACAGGGTACACAGGTGTAACTAGAAGCCTCGGTGTGATCACATCAAGAGTCACAAGCA CAAGTCTACCTGGAAAGTCCACAGTAGTGCATAGCACCCCTGCTCAGCCATTGTCTGCACATTCTCAGAG TCACCAGACGTATGGCACGGGGACACCCAGCATGTCTCAAACCAGCATCCTCCCAGACATGACCTCAGAA AAGCGTGTTGCCTCTTCACCTGGACCCACTGTGACAGAGTCCTTTTCTCATGTGTCATCCTCCTCAGGAT TGACTACGAAGACAGACAATGACAGAAACACAGCAGTCTCAGCCACCTCCTCGACCCTTACTAGCCCCAG CCCAACCACAGCCTCCAGGTCAACAGTGCCCCTGCCTTCTCTTTTACCAGATCAGGGCATTTCCCTCTTC CCCTACGGGTCTGAAGTCGGAGACCAGAATTTGTTTGCCAGGACTGTGGATTTTAACTCACCAATCTTCA AGATCCTGATTGGCTTTCCCCTCGGTTCCTCCCTGCGGGATTCCTTCTACGTTACAGATAATGGTCAGAT CATTTTCCCAGAGTCAGACTATGACGTCTTCTCCTACCCCAACCCACCTCAAAGAGGCTTCACCGGAAGG GAGCGCGTGGCCATGGTGGCCCCGTTCTGGGCTGATGCTGATTTCTCTAGCTCCAGGGGAGCCATATTTT ACCAGGAATATGTCACATTTTACAATGAACACCATCAGCTAATCCGGGAAGTGGAGACTTTGATTAATGA TTTCACAAGTAGCTGGGGCTACAGAGCCAAGTGGACACTGAAGGTCACCTGGGTCAATGTTCCTGCCTAT ACTGCCCAAGAGAGCTTTGGGACCAATACCTACCAAGCCATCCTCTCCACAGATGGGAGCAGGTCCTATG CCCTGTTTCTCTACCAGAACAGTGGCATGAGGTGGGATGTGACCCAGGAACCATACAACCGGGTGCTCAT GGGCTTCTCCAGTGGAGATGGGTATTTTGAAAACAGCCCGCTGACATTCCGGCCAGCAATGGAGAAGTAC CGTCCAGATCGATTCCTGAATTCCAAATTAGGCATCCGGGGACTGCAGGTCTACAGGCTGCACAGGGAGG AGCGGCCCAACTACCGGCTCAAATGTCTGCAGTGGCTGGAGAGTCAGCCTCAGCAGCCCAGCTGGGGCTG GAGCTCAGTTTCCTGCCCTTGCTCCTGGCAGCAGGGACAACGGGACTTTCGGTTCAGGCCCATCAACCCA GGTTGGTGGGACAGGCAGCTGTGCAGCTTCTCCTCCGGGCGAGGGGGTGTGTGCTGCAGCTATGGTGCCT GGGGAGAGTTTCGAGAAGGCTGGAGAATGCACAGTCCTTGGCAGTTTGATGAGGAGCAGGAGGCACAGAA CTGGTGCTGCCAATGGAACGACAAGCCCTCGTTCTGTGTCTGGTACCAGCTTAGGCGGCCCCGTGTCAGC TGTGCTGGGTACAGGCCCCCACGGCCCGCTTGGACATTTGGTGATCCCCACATCACCACTTTGGATAATG CCAACTTCACCTTCAACGGGCTGGGAGACTTTCTGCTGGTCCAGGCCCAGGACAGAAATTCTTCCTTCCT GCTGGAAGGCCGCACTGCCCAGACTGGCACTGCCAAGGCCACTAACTTCATTGCCTTTGCGGCTCAATAC AACACCAGCAGCCTGAAGTCTCCCATCACGGTTCAGTGGTTTCTTGAGCCCAGTGACAAAATCCGAGTCG TATACAATAACCAGACTGTGGCCTTTAACACCAGAGACACTGAAGTCTTACCCATATTCAATACCACCGG TGTTCTGCTGACCCAAAATGGCTCCCAAGTCTCAGCCAACTTTGATGGGACAGTGACTATCTCTGTGATT GCTCGCTCCAACATCCTTCATGCCTCCTCCAGCCTATCAGAGGAATATCGAAACCACACAGAGGGCCTTC TGGGAGTGTGGAATGACAATCCAGAAGATGACTTCAGGATGCCCAATGGCTCCACCATTCCCTCAAACAG CTCTGAGGAGACCCTTTTCTACTATGGAATGACATGGCACGTCAACGGAACAGGCCTCCTTGGGATAAGG GCAGACCCGCTGCCTACCAAGTTCACCCCCATCTTTCTGTCTCAACTGTTGAATCAGAGTGCCTCAGGTG AAGACTTGGCCTCTGGGTGCAAAGGCGACAGAAAGTGCATGTTTGACATCCTGGCCACGGGAAACAGAAC TATTGGACAAAGCACCAATTCCATCCTTAATGAGTTCCAACATATGAATGACACCCTCAATCAGTACCCA CCCTCTATCAACTGCAGCAGCAAGATTCAAGCCTACAAGGGGCAGACAGTGACCACTGAGATCACCAGCA ACTCTAAGGATGCCACACTCAGCCTCTCCAAGAAATGTAGTGGCTTTAAGCTCTTTGAAAACGGGAGTTT GCAGTGGACACCAACATCCCCAGAAGCGTGTACCCTGGAGATTCTAGCGAGGGATGTCAGGACTAACTTG TCATGGGTACTCCAGCCCAAGACCGTGGCTTGCTTCTGCAGTAAGGAGGAGCAGTGTTTGTACAATGAAA CCAGCAAAGAGGGCAATTCTTCCCTTGAGGTGACCAGCTGCAAGTGTGATGGGGACACCTTTGGCCGCTT GTGTGAACGCTCCAAGGACCCGTGTGATGAGCCATGCTTCCCTAACGTGAACTGCATTCCTGGGAAGGGC TGTGAGGCCTGCCCTCCAAACACAACTGGAGATGGACGTCATTGTGCAGCTCTGGAGGACTCCTGCCCAA ACCGTTCCTGTCCCATGAATTACTGCTATAACAATGGCCATTGCGACATCTCTGAGGCTCCAGGCTGCCA GCCCACTTGCACCTGTCCCCCTGCCTTCACTGATAACCGCTGCTTCCTGGCTGGGAACAGTTTCACTCCC ACCATCTCTATGGAGCTTCCCTTAAGGACCATCGTGCTCTCTCTCAGGGAGGATGAAAATGCCTCCGCCG CTGACGTCAACGCCTCGGTGGCGAACATACTAGAGAACCTGGACATGCGGGCTTTTTTCTCCAACAGCTT AGTGGAACTGATACGAACCTCTCCCGGAGCACAGCCCTCGAGCAAGTCCATTCATCACTGGAAGGTCACC TCCCACTTCAAGTACCGTCCCAGGGGCCCGCTCATCCACTATCTGAACAACCAGCTGATAGGCGCCGTGA TGGAGGCCTTCCTCCTGCAGGCTCGGCAGGAGAGGCAGAAGAGGAGTGGAGAAGCCAGGAAGGACGTCCA CTTCTTCCCCATCTCGAGGGCAGATGTCCAGGACCAGATGGCTCTGAACCTAAGTATGCTGGAGGAGTAC TTCACATGTGATGGCTACAAAGGCTACCACCTGGTCTACAGCCCACAGGATGGTGTCACCTGTGTGTCCC CATGTAGTGAGGGCTACTGTCACAATGGAGGCCAATGCAAGCACCTGCCAGATGGGCCCCAATGCAGCTG CGCATCCTTCACCATCTACTCATCCTCGGGCGAACACTGTGAGCACCTAAGTGTGAAACTTGGGGCATTC TACGGGATCCTCTTCGGAACCCTGGGCGCCCTCTTGCTACTGGGGATCTTAGCATTTATGATCTTTCACT TCTGCGGCTGCTCCAAGAACAAGTTCTCCTACCCTTTGGACTCAGAATTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_080457
Insert Size	10413 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080457.3, NP_536705.3
RefSeq Size	11277 bp
RefSeq ORF	11973 bp
Locus ID	140474
UniProt ID	E9Q7Q0
Gene Summary	The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]

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