Tnxb (NM_031176) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Tn-mhc; Tnx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229766 representing NM_031176 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTCAGTGCACCCCGTCTATTCTGACCATCCTGGTGTTGCTTGGCTTAGCCAGAGCAGGCCCAT TCTCTCCTCGGTCTAATGTGACACTTCCGGCCCCACGGCCCCCTCCCAAGCCCGGTGGTCCTTCTTCTCA GCTGTACGAGCATACTGTGGAAGGAGGAGAGAAGCAGGTGGTGTTCACCCATCGCATTAACCTGCCCCCG TCAGCCGGCTGCGGCTGTCCCCCGGGCTCTGAGCCCCCGGTGCCTGCTTCAGAGGTGCAGGCCCTGAGGG TCCGCCTGGAGATCCTGGAGGAGCTGGTAAAAGGGCTTAAGGAGCACTGCACAGGAGGATGCTGTCCCAC TGCGGCCCAAGCTGGCACAGGCCAGACTGACGTGCGCAGCCTCTGCAGCCTCCACGGTGTCTTTGACCTG AGCCGCTGCGCCTGTTCCTGTGAGCCTGGCTGGGGTGGACCCACCTGCTCAGACCCTACGGACACAAAGA CGCCCACCTCATCGCCGCCCTCCAAGTCCTGTCCCGAAGACTGCAACGACCAGGGACGCTGCGTCCGAGG ACGCTGCGTGTGCTTCCCCGGCTACAGTGGCCCCAGCTGTAGCTGGCCCTCTTGCCCTGGAGATTGTCAG GGGCGCGGGCGCTGCGTGAAGGGCGTGTGCGTGTGTCGCGCGGGCTTCTCAGGCCCTGACTGCAGCCAGC GCTCTTGCCCTCGCAACTGCAACCAGAGAGGACGCTGCGAGGAAGGACGCTGCGTGTGTGACCCCGGCTA CTCCGGTGAGGACTGTGGGGTGCGGAGCTGTCCCAGGGGATGCAGCCAAAGAGGCCGCTGCGAGAACGGC CTCTGCGTGTGCAACCCCGGCTACTCTGGCGAGGACTGTGGGGTGCGGAACTGTCCCAGGGGCTGCAGCC AGAGGGGTCGCTGCGAGGACGGCCGCTGTGTGTGCGATCCCGGCTATAGTGGCGAAGATTGCAGCATGAG GACCTGCCCATGGGACTGTGGCGACGGAGGGCGCTGCGTGGACGGTCGCTGCGTGTGCTGGCCCGGGTAC TCGGGCGAAGACTGTAGCACGCGGACGTGCCCGCGTGACTGCCGTGGCCGGGGCCGCTGCGAGGACGGGG AATGTATCTGTGACGCAGGCTACAGTGGCGACGACTGCGGTGTGCGGAGCTGCCCCGGGGACTGTAACCA AAGGGGCCACTGTGAGGATGGCCGCTGCGTGTGCTGGCCCGGGTATACTGGAGCGGACTGTAGCACGCGC GCCTGCCCACGGGACTGCAGGGGCCGCGGGCGCTGCGAGGATGGCGTGTGCGTGTGCCATGCGGGCTACA GCGGCGAGGACTGCGGGGTGCGCAGCTGCCCCGGGGACTGTCGCGGCCGTGGGAACTGCGAGAGCGGCCG CTGCGTGTGCTGGCCTGGGTACACAGGCCGAGACTGTGGCACGCGTGCCTGCCCCGGTGACTGTCGTGGA CGCGGGCGCTGCGTGGACGGCCGCTGCGTGTGCAATCCGGGCTTCACTGGCGAGGACTGCGGGAGCCGTC GGTGTCCTGGGGACTGTCGCGGACACGGCCACTGCGAGAACGGCGTGTGCGTGTGCGCTGTGGGCTACTC TGGCGACGACTGCAGCACTCGCAGCTGTCCCAGCGACTGCAGAGGCCGCGGCCAGTGCCTGAATGGGCTC TGCGAGTGCGACGAAGGCTACTCGGGTGAGGACTGTGGCATAAGACGGTGTCCCCGCGATTGCAGCCAGC ACGGCGTGTGTCAGGATGGCTTGTGCATGTGCCACGCAGGCTATGCGGGCGAAGACTGCAGCATTCGCAC CTGCCCGGCCGACTGTCGCCGGCGTGGCCGCTGTGAGGACGGGCGCTGTGTGTGCAACCCAGGCTACACC GGGCCCGCATGTGCCACTCGAACCTGTCCAGCTGATTGTCGTGGCCGTGGGCGCTGCGTGCAGGGAGTGT GCATGTGCTACGTTGGCTACAGCGGTGAGGACTGTGGACAGGAAGAGCCTCCTGCTAGTGCCTGCCCTGG GGGCTGTGGGCCGCGGGAACTGTGCCGCGCCGGACAATGCGTGTGTGTTGAGGGCTTTCGAGGCCCAGAC TGTGCCATCCAGACGTGCCCAGGTGACTGTCGCAGCAGGGGAGAGTGTATCCAGGGCAGATGCGTCTGCC AAGAAGGCTACGCTGGGGACGACTGCGGGGAAGAGATACCAGCCATCCAGAACATGAGGATGCATCTTCT AGAGGAGACAACAGTCCGGACAGAGTGGACACGAGCTCCCGGTCCTGTGGATGCCTATGAAATCCAGTTT ATCCCCATGACCGAGGGGGTGAGTCCACCATTCACAGCTCGGGTGCCCAGCTCTGCCTCAGCCTATGACC AGCGAGGACTGGCGCCTGGACAGGACTACCAGGTCACTGTTCGTGCTCTACGAGGGACCAGCTGGGGTCC TCCTGCCTCTAAGACCATCACTACCATGATCGATGGTCCTCAGGACCTCCGGGTGGTAGCTGTGACCCCG ACCACACTGGATCTTAGCTGGCTGCGCCCCCAGGCTGAGGTGGACCGATTTGTGGTGTCCTATGTCAGTG CTGGAAACCAAAGGGTGCGACTGGAAGTTCCCCCTGAGGCAGACAGGACTCAGCTAACCGATCTGATGCC TGGTGTGGAGTATGTGGTGACTGTCACTGCAGAAAGAGGCCATGCAGTCAGCTACCCAGCTTCTATCAGG GCGAATACAGACAGGGCTCAGGAACAGCCTGGGAAGCCATCAGTCCAGCCACGCCTGGGTGAACTGACAG TGACAGGCCTGACCTCTGATTCCCTGCTCCTGCACTGGACTGTCCCAGAGGGGGAGTTTGACTCCTTCCT GATCCAGTACAAGGACAAAGACGGGCCCCAGGCGATATCCGTGGAGGGACCCCAGCGTTCTACACCCATC TCAGGCTTGGAACCAGGACGCAAGTACAAATTTATCCTGTATGGACTCATCGGCAAGAAGAGGCATGGCC CACTTATGGCTGAAGCCAAGATCTTGATTCAGAGTGACCCTGACCCAGGATCCCCACCCCGCCTGGGAGA GCTATGGGTGACAGACCCCACTCCACACTCATTGCACCTCTCCTGGACTGTTCTCGGAGGCCAGTTCGAC TCCTTCGTGGTCCAGTACAGAGACAAGGAAGGCCAGCCCCGTGTGGTACCTGTGGAGGGGCCTGATCGCT CAGTGGTGATCTCCCCACTGGACCCCAATCGCAAGTACAGGTTCACTCTGTTTGGGATTGCCAATAAGAA GCGGTACGGGCCCCTTACTGCTGATGGCACTACTGCTGCAGAGACGAAAGAGGAGTCCTCAGAGCCGCCC CGCCTCGGCGAACTGACTGTGACTGGTGTGACCCCAGACTCCCTGCGCCTGTCCTGGACAGTGGCCCGGG GTCCCTTCGACTCCTTTGTAATCCTTTACAAGGATGCACAGGGCCAGCCCCAGAGTGTACCCATCGAAGG TGATGAGAATGAGGTCACTGTCCCCGGCCTGGAGTCCAACAGGAAGTATAAGATGAACCTCTACGGGCTT CGTGGCAGGCAGCGTGTGGGGCCGGTATCAGTGGTGGCCAAAACTGCCCCACCAGATGTTGTAGACCAGC CCCTCAGCCCCCCAGAGCCCAGCACAGAGGCCCCAGAGCCCCCCGAGAAGCCACTCCTGAGAGAGCTGAT CGTGACAGACACCACACCCAACTCCCTGAGCCTCTCCTGGACAGTCTCCGAGGGACAGTTTGACCAGTTC GTGATCCAGTACAAGAACGGGGATGGGCAGCCCAAGGTGGTGCGGGTGCCAGGACACGAGGACCAGGTCA CCATCTCCGGCCTGGAGCCAGACCACAAGTATAAGATGAACCTGTATGGCATCCATAGTGGCCAGCGTGT GGGCCCCGTGTCTGTTGTTGGTGTGACAGCTGCAGAGGAAGAACCCCCCAGCCCCACAGAGCCCAGCACA GAGGCGCCAGAACCCACTGAGGAGCCGCTCCTGGGAGAGCTGACAGTGACAGGATCGTCCCCAGACTCCC TGAGCCTCTCCTGGACCGTCCCCCAGGGAGACTTTGACTCCTTCACTGTCCAGTACAAGAGCAGTGATGG GCGGCCCCAGGTGATGCGTGTCGGGGGCCAGGAGAGGGAGGTCATCGTGAGAGACCTGGATCCTGGGCAC AAATACAAGATGAACCTGTATGGACTCCATGAGGGGCATCGTGTGGGCCCTGTGTCCACTGTGGGCACAA CCGACAAACAACCAGAAGAAAAGCCCCCCCTTGAGCCACGCCTGGAGGAGTTGACAGTGACTGGTGTAAC CCCCAATTCTGTGAGCCTCTCGTGGATAGTTCCTGAGGGACAGTTTGACTCCTTCGTGGTCCAGTACCAG GACAGGGATGGGCAGCCCCGGGAGGTACCACTGACAGCAGAACAGCGTGAGGTCACTGTCCTTGACTTGG ACCCTGCAAGAATATACAAAATGAACTTCTACGGGCTACACGGTAGACGACGTGTGGGCCCCCTCTCCGT GTCAGCAATGACAGCACCAGCTCCAGCCACCGAGGCCTCCAAGCTGCTCCCAGAACCACGACTGGGGGAA CTAACAGTGACAGATGTGACCCCGGACTCAGTGGGCCTCCTGTGGACTGTCCCTGAGGGTGAATTCGACT CCTTCACGGTCCAGTACAAGGACAGGGATGGCCAGCCCCACGTGGTGTCTGTGGCAGCAGACCTACGGGA GGTCACCATCCCAGGCCTGGAGCCCTCTCGCAAGTACAAGTTCCTGCTCTTTGGGGTCCAAGATGGGAAA AGACGCAGCCAGGTCTCTGTAGAGGCGAAGACAGCTACCCATGATGACACCAGCCCAGGGGCCCCTCCCC GCCTTGGAGAGCTGTGGGTGACGGATCCTACCACGGACTCTCTGCGCCTATCCTGGACAGTTCCCGAGGG CCACTTTGACTCTTTTGTGGTCCAGTTCAAGGACAAAAGTGGGCCCAGGGTGGTGCCAGTAAAGGGTCAA GAGCGGACAGCCACTATCACGGCCCTGGATGCTGGCCGCAAGTACAGATTCCTCCTCTATGGGCTTCTGG GAAGGAAACGCTTTGGCCCCCTCACTGCAGAGGGCATAACAGAGCCCACGAATGCTGCGGACCACACAAA ATCTCGACTGGGAGAGGAACTGCGGGTGACTGGTGTGACTCAAAACTCCGTGGACCTCTCCTGGACAGTC TCCGAGGGCCAGTTTGACTCCTTTGTGGTCCAGTACAAGGACAGGGATGGGCAGTCCCAGGTGGTGCCTG TGGACGGAAGCCATAGAGAGGTTAGAGTCTCTGGTCTAGACCCAGGTCGCAGGTATAAGCTGCTACTCTA CGGCCTCCGAGACAGCAAGCGTGTGGGTCCCCTTTCAGTCATTGCTGTGACCGACCCCAGAGAAACAACA GAAGCTTGGACAGAGGTCCCCACAACTTCAACTCCTGCAGCTGTGCCCCGCCTCGGGGAGCTGAGAGTGG AGGAAGCCACGCCTCACAGCCTGCATCTCTCCTGGGTGGTGACTGAGGGAGAATTTAACTCCTTCGAGGT CCAGTACACTGACAGAGATGGGCAACTCAGAGTGGCCAGTACAGAGGGTGACCAGACTGACATTACCCTT ACTGGCCTGGAATCGAACCACAGATACCTGATAACCCTATATGGCTTCCATGATGGGCAGCCTGTAGGCT CTGCTCAAATTGAGGCCCTGACAGTGCCGTGGGAGGAAGAAGAGGAGGAAGAGGAGTCCACAGAGCCACC CACCATGACTCCTGAGCCCAGGCCACAACTGGGAGAGCTGACCGTGACAGATGCCACCCCTGACTCTCTG AGCCTTTCCTGGACAGTCCCCGAGGGACAGTTTGACCAGTTCGTGATCCAGTACAAGAACGGGGATGGGC AGCCCAAGGTGGTGCGGGTGCCAGGGCATGAGGACCAGGTCACCATCTCTGGCCTGGAGCCAGACCACAA GTACAAGTTGAACCTGTATGGCATCCACAGTGGTCAGCGTTTTGGCCCCGTGTCTACTGTTGGGGTGACA GCTGCAGTGGAAGAGCCCCCCAGCCCCACAGAGCCCAGCATAGAGGCCCCAGAGCCCACTGAGGAGCCAC TCCTGGGAGAGCTGACAGTGACAGGATCGTCCCCAGACTCTCTGAGCCTCTCCTGGACCGTTCCCCAGGG AGACTTTGATTCCTTCACTGTCCAGTACAAGGACAGTGATGGGCGGCCCCAGGTGATGCGTGTCGGGGGC CAGGAGAGGGAGGTCATCGTGAGAGACCTGGATCCTGGGCGCAAGTACAAGATGAACCTGTATGGGCTCC ATGAGGGGCGTCGTGTGGGTCCCGTGTCGACTGTGGGTGTGACTGCCTCTCTGACCACAGAGCCCCCCAT AGAGCCCCGCCTGGGAGAGCTGGCTGCAGTAGAAGTGACTTCCAGTACAGTGCACCTGTCCTGGACTGTG GCCCAGGGCCCCTTCGACTCCTTCCTGGTCCAGTACAAGGACACACAGGGGCAGCCCCAGATGGTGCCTG TGAGTGGAGACCTGGGTGAGGTCACCGTCTCAGGTTTGGAGCCAGCCCGAAAGTACAAGTTTCTACTCTT TGGACTCAAGGATGGGAAGCGCCATGGCCCTGTCTCCACAGAAGCAAAGACTGTCTCTGATGCAAAGCTG CTTCCTCGTCTTGGGGAGCTCACCGTGACAGACACGACCCAAAACTCCGTGGGTCTCTCCTGGACTGTTC CTGAGGGTGAATTCGACTCTTTCTTAGTCCAGTACAAGAACAGGGGAGGGCAGGTCCAGGTCGTGCCTGC AGCAGCAGACCAACGTGAGGTCACCATCTCCAGCCTGGAGCCCAATAGAAAATACAGGTTCCTGCTCTTC GGCCTCATAGGCCTCAAACGGCTGGGGCCTGTCTCCGTGGAGGGCACCACGGCTCCCGTGGAGAAGACAC CACAGCCCCGCCTCGGTGAACTGTCAGTGACAGAGGAGACCTCTGACTCCATGCACCTCTCTTGGTCTGT GGCCCAGGGTCCCTTTGACTCCTTCCTGGTCCAGTACAAGGACAGGGATGGGCAGCTCCAGACGGTGTCC TTGGCTGCAGACCAGCGAGAGGTCACCATAGAGGGTCTACAGCCAGGCAGGAAATACAAGTTCCTGCTCT ATGGGCTCACTGGAGGGAAGCGCCTGGGACCCATCTCTGCCTTGGGAGTGACAGCCTCAGAAGTTGACAT ATCAGCCCCAGACCACACTGAGCCCCCTCATGCTCCCGGCCTGGGGACGCTGGCAGTGACAGAGGCAACC CCAGACTCCCTGCGACTCTCCTGGGCTGTGGCTCGTGGCCCCTTTGACTCCTTTGTGGTCCAGTACCAGG ACACAGAGAATGAAACCCAGGCCCTGCTTGTTGATGGAGACCAGGACAAAGTCCTCATCTCAGGCCTGGA ACCCAAAACTTCCTACAAGTTCTTCCTCTATGGCCTCCGTGAAGGGACACGCCACGGACCTGTGTCTGTA GAGGGCACCACAGATCCAGTTCCTGCTGGTCAGGGCCCAGGGGACCCCGGGCCCCGCCTGTCCCAGCTCT CAGTTTCAGATGTGACCACCAGTTCTCTGCGGCTAAACTGGGAAGCCCCGCTTGGGGCCTTTGACTCTTT CCTGCTACGCTTTGGGGTCCCCCTACTGAGTACCCTGGAGCAGAATCCTCGCACTCTGCAACAGCGAGAG CTTATGGTGCCAGGCGTGCGGCGATCCGCCATGCTCCGGGACCTGCGTCCTGGGACCCTGTATAGCCTTA CTCTGTATGGGCTGCGTGGGCCACACAAGGCAGACAGCATCCAGGGCACTGCCCGCACACTCAGCCCAGT TCTGGAGAGCCCCCGGGACCTGCAATTCAGCGACATCGGGGAAACATCAGCCAAAGTCAAATGGGTGCCG CCAACGTCCAGGGTGGACAGCTTCAAAATCTCCTACCAGCTAGCAGATGGAGGAGAGCCACAAAGTGTGC AGGTGGATGGCCGGACCCAGACCCAGATCCTCCAAGGGCTGATTCCAGACACTCGCTATGAGGTGACTGT GGTCTCCGTCCGTGGCTTTGAGGAAAGCGAGCCTCTCACAGGCTTCCTCACCACAGTGCCTGATGGTCCC ACCCAGCTACGGGCATTGAACTTGACTGACGGATCTGCCCTTCTACACTGGAAACCCCCCCACAAACCAG TGGACAAATATGACGTCGAGGTCGAATCCCCTGGGGCCCCACCCCTTCAGGCCTCAGCACCTGGCAGCGC TGTGGACTACCCACTGACTGACCTGGCACTCGACACCAATTACACAGCCACCGTGCGTGGTCTCCGGGGT CCTAACTTCACCTCCCCAGCCAGCATAACCTTCACCACAGGGTTAAAGCCCCCCCAGGACTTGGAGGCCA AGGAAGTGACTCCTCGCACGGCCCTGCTCACTTGGACTGAGCCTGAAGTCCCACCCACAGGCTACCTGCT CAGCTTTGACACCCCTGGAGGACAGATTCAGGAAATCCTGCTTCCAGCAGGAACCACCTCGCACAGGCTT CTGCGCCTCTTCCCCTCCACCTTCTACAGTGCCCAGCTGCGGGCCATCTGGGGCGAGAGCCTTACACCTC CCGTGCTCACTTCCTTTACTACTGGTGGGCTGCGGATCCCCTTCCCCCGGGACTGTGGGGAGGAGCTGAA GAATGGGCCCAGTGCCTCAAAGACCACCACCATCTTCCTCAATGGCAACCGTGAGCGGCCTTTGGATGTG TTCTGTGACATGGAGACTGATGGAGGTGGTTGGCTGGTGTTCCAGCGCCGCATGGATGGACAGACGGACT TCTGGAGAGACTGGGAGGAGTACGCCCATGGTTTTGGGAACATTTCCGGGGAATTCTGGCTGGGCAACGA GGCCCTGCACAGCCTAACACAGGCTGGAGACTATTCTCTGCGTGTGGACCTGCGGGCCGGAAAGGAAGCC GTGTTCGCCCAGTATGACTTCTTCCGTGTAGACTCAGCAAAGGAGAACTACCGCCTACACCTTGAGGGCT ACCATGGGACTGCAGGCGACTCTATGAGCTACCACAGTGGCAGTGTCTTTTCTGCCCGTGATCGAGACCC CAACAACTTGCTCATCTCTTGCGCTGTCTCTTATCGTGGGGCTTGGTGGTACAGGAACTGTCACTACGCC AACCTCAATGGGCTCTATGGGAGCACAGTGGATCACCAGGGAGTGAGCTGGTACCACTGGAAGGGCTTCG AGTTCTCTGTGCCCTTCACGGAAATGAAGCTGCGACCCAGAAACTTCCAGGTCCCCACCAGGGGCACCTG A AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_031176
Insert Size	9381 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031176.2, NP_112453.2
RefSeq Size	9569 bp
RefSeq ORF	9381 bp
Locus ID	81877

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