Wnk1 (NM_001199084) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229756 representing NM_001199084 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCAGTCCATGGCGCATCCGTGTGGGG GGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACC ACCTCCCACCTGTCCACCGAAAGTAGCCATTTCCCAACGACGTAAGAGCACCTCCTTCCTGGAAGCCCAA ACTCGCCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTAGCAGCCCAACTA ACTGGACACCAGAGGCCATAGTTATGTTGGGTGCTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGAT GCAGGTCCAACCTGTGTTTGAGCCAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAA GAAGCTCACTATTTTATTCCTCAGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCAGGTGGCAG GACAGTATGAGGGCATTTCATACAACTCACCAGTACTGTCAAGTCCTATGAAACAGATATCTGAACAGAA GCCAGTGCCGGGGGGCCCTGCCTCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTGGTAGGA CACCTTCAGAATTTAAGATTAGATTCTGGACCAAGTCCAGCATCACCCCTCTCTAGTATTTCTGCGCCTA ACAGTACAGATGCTACACATCTGAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAAC TAATAGCAATGAGAACAGAAGCAACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCTTCAGGAGAA GGAGGTGGGATTTTACCTCAACGTGTTTACCGAAATCGGCAGGTTGCAGTGGACTCAAATCAGGAAGAAC TGTCTCCTCAGTCAGTTGGATTACATTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCATGC CCCCGAATTGACCATTTCTGTGGTAGAACCTATGGGACAAATCTGGCCAATAGGAAGCCCAGAATATTCC AGTGATTCCTCTCAAATTACTTCTTCAGATCTCAGTGATTTTCAATCACCTCCCCCTACAGGGGGGACAG CTGCACCTTTTGGCTCTGACGTCTCATTGCCCTTTATTCGCCTGCCTCAGACAGTGTTACAAGAATCCCC ACTATTCTTCTGTTTCCCCCAAGGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGAGGA TCTACACTCCATCCACAGGCACAGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTGGCTGGGGTTCTAT CTTCACAACCTATCCAACATCCTCAGCAGCAGGGAATACAGCCAACTGTTCCTTCTCAACAGGCAGTACA GTATTCACTTCCACAAGCAGCATCTTCCAGTGAAGGTACTACTGCACAGCCAGTGAGTCAGCCTCAAGTG TCAGCTGGAACACAGGGCTTCCCATCTCGACTGCCACCACAATACCCAGGAGATTCAAATATTGCTCCCT CTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTAGCCCCTCCTTCTATGCCGACAGAAGCATT GGCCACCCAAGGTTACTTTCCTACAGTAGTGCAGCCTTATGTGGAATCAACTCCTTTGGTTCCTATGGGC AGTGTAGGAGGCCAGGTTCAAGTGTCCCAACCAGCAGTGAGTTTAACGCAACAACCCCCAACTACATCCT CCCAGCAAGCAGTTCTGGAGAGTACTCAGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAATAAC ACAGTCACAACCTACTCAGCCTGTTCCATTGGTTACCTCTGCAGACAGTGCACATTCAGACGTTGCTTCT GGTATGAGTGATGGCAATGAGAATGCTCCATCATCCAGCGGGAGGCATGAAGGGAGGACAACAAAACGGC ATTATAGAAAATCCGTAAGAAGTCGCTCTCGGCATGAAAAGACTTCTCGCCCAAAACTGAGGATTTTAAA TGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATCGGAAGATGGTTACATTT AAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAG CAATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAGTGA GGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTGCAAGGAAAGGATGACTATGGCTTC CCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAATAG GTGTTCCTACCAGTTCTTTAACGCAAGTTGTTCATTCTGCTGGAAGACGGTTTATAGTGAGTCCTGTACC TGAAAGTAGGTTACGAGAATCAAAAGTTTTCACCAGTGACATATCAGATCCAGTTGTGGCCTCTACATCT CAGGCCCCTGGAATGAACTTATCTCACTCTGCTTCATCCCTCAGTCTCCAGCAGGCTTTTTCTGAACTGA AACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATCACATGGCGGGACCAACATT TTCTCCCTTTTTGGCTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACCGTCAGTATCAGTCCCT ATAACAAGCAGTCCTCTTAATGACATTTCCACATCAGTGATGCAGTCTGAGACTGCACTGCCCACTGAAA AAGGCATCGTGGGAGTTACCACTACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACAATGTCTGT GTCTGAGTCACCAACATCTTCCAGTGCGGTTTCAAGTTCTACAGTACCTGCAGTTGTCACTGTGTCCACA CCATCCCAGCCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCAAGTACAGGCAGTTTTCCTCCTGGAA CATTTTCTACAACTACAGCTACTACAATGGGTAGTGTGGTGGCCCCAGATGCTAAGCCTCCAACTGTATT ATTGCAACAGGTAGCAAGCAATACTGCTGGGGTTGCCATAGTGACATCAGTCTCTACCACCACTCCATTC CCAGGCATGGCTTCACAGCCATCCCTTCCACTTAGTAGCAGCACCTCTGCTCCTACTCTAGCTGAAACAA TGGTGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTG TGCGCCATCCTCTTCCTCCTCCTCTGGAACAGCTGTGTCTACTTCTGTTTCTCAGCCTGGTATGGTTCAT CCTTTGGTCATCTCATCAGCTGTAGTTTCTACACCTGGCCTCCCCCAACCAGTAGTACCTACTTCTACAC CTTTATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGTTAATGTGCCTGCTGTACAGCA AACACTCATTCACAGTCAGCCTCAACCAGCTCTGCTTCCCAACCAGCCTCACACACACTGTCCTGAAATG GATGCTGATACCCAATCCAAAGCTCCTGGGATTGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCTC TCTTTAGTGAACACAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTAGAGACACCTTTAGTAGTAGA GACGACTGTCACACCGGGCATCACTACCACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGT ACTTGCTTACCACCAACCAGTTTACCATTAGGAGCAGCTGGCATGCCAGTTATGCCAGTAGGCACACCTG GGCAGGTTTCTACCCCAGGCACACACGCTTCAGCCCCAGTCGGCACTGCAACAGGAGTAAAACCTGGAAC AACTCCCCCAAAGCCAACTAAGACTGTGGTGCCACCAGTGGGTACTGAACTTTCAGCCGGTACTGTACCC TGTGAGCAGCTGCCACCTTTTCCTGGACCTTCTCTAATTCAGTCCCAGCAACCACTAGAAGATCTTGATG CTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTACAGTGGCACCTGCAGTTGGTCCTTTGTCTACGAT GTCTTCAACAACAGTTACAGAAGCAGGGACTCGGCTTCAAAAGGATGGCACAGAAGGTCATGTCACAGCA ACTAGTTCAGGAGCTGGTGTTGTTAAGATGGGGCGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGA AAGAACGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGCACATCAGAGTCATCAGT TCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAATTAGTATTTCTGGCATC TCCTTAGATGTCCCAGACAGCACCCACAAAGCACCTACCCCAGAAGCAAAGTCAGATGCAGGACAGCCTA CCAAGGTGGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGA GGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTCCTTTCAGAGATTCAGAACAAACT GTTATTCCTGCTGTGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCATTTGAATGGGCCAT CTTCAGACCTGGAGGCTGCCTTTCTAAGTAGGGGTACAGAGGATGGCTCAGGTAGTCCACACTCTCCCCC TCACCTGTGTTCAAAGAGCCTTCCCGTCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCA TACATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTACGACGACTACGAG AAAAACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACTAA ACTGGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCGGGGAGAAGAAGGAGACCC ACTAAAAGCAAAGGCAGCAAGTCTAGTCGCAGCAGCTCATTGGGCAATAAAAGCCCACAGCTTTCAGGCA ACCTGTCTGGTCAGAGTGGAACTTCAGTCTTACACCCCCAACAGACCCTCCATCCCGCAGGAAACACCCC CGAGACTGGGCACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCC TTTACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAATA CCGTTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCTATGACTTCCAGTAGGAAGGG CACATTCACAGATGACCTGCACAAGTTAGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGG AGAGGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGC TGTGTGTTCCCATGACCTCAAACCTGGGTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGG TCACTTCACCAAATCCATGTGTCCCCCACAGCAGTATGGTTTTCCACCTGCCCCATTTGGCACTCAGTGG AGTGGAACAGGTGGTCCAGCACCGCAGCCACTTGGCCAGTTCCAACCTGTAGGAACAGCATCCTTGCAAA ATTTCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAATCTACGGACCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199084
Insert Size	7908 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199084.1, NP_001186013.1
RefSeq Size	11346 bp
RefSeq ORF	7908 bp
Locus ID	232341
UniProt ID	P83741
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) represents the longest transcript and encodes the longest isoform (5). This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant described in Figure 2F of PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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