Cacna1c (NM_001255998) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229720 representing NM_001255998 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGGGCCTTTGTTCAGCCATCCACACCCCCATACCAGCCACTCTCCAGTCACTCGTCTGAGGAAA CAGAGAGGAAGTTCAAGGGCAAGGTGGTACATGAAGCTCAACTCAACTGTTTCTACATTTCTCCTGGAGG TTCCAACTATGGGAGCCCACGCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCC GAGCACATCCCTACTCCAGGGGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGC TCATGGGCAGTGCTGGCAACGCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGG GAAACCCAAGAAGCAGGGGGGCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAG AACCCTATCCGGAGGGCGTGCATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTA TTTTTGCCAATTGTGTGGCCTTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTC CAACCTGGAACGAGTGGAGTATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCC TACGGACTTCTCTTCCACCCCAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGG TTGTAGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAA AGGAGCTGGATTCGACGTGAAGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGA GTCCCAAGTCTCCAGGTGGTCCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTC TTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGAC CTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACA GGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCA CCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGA CGTGCTGTACTGGATGCAAGACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATC TTTGGATCCTTTTTCGTTCTAAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGA AAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTA CCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAG CCTCGAAACAGAGGCGCTCCAGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACT CTACAGAAACCCATGTGAGCATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGG TGACATCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTAC TGGCGCAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGC TCGTGATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCT CACAGAAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATG TACAGCCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGG GCATCCTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGT GCGGTTGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTG AACTCAGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGG GGATGCAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTT CCCGCAGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGG ATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCT GTGGAAATTATATCCTACTGAATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCT GACCTCAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAA AAGAAACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCA TTACAGCCGATGGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGA GGATAAGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTG GGGCCACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTG CATTTTTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTT CACCAACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCAC ACCTCCTTCAGGAACCATATTCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTG CTCTAAAGATGACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCT GGACCTGCTGGTGGTTAGCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAG ATTTTACGAGTGCTGCGAGTCCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGG TTCAGTGCGTGTTTGTGGCCATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCAT GTTCGCCTGCATTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACG GAGGCAGAATGCAAGGGTAACTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTC GAAGCTGGGAGAACAGCAAGTTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTC CACCTTCGAAGGGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATC TACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGA ACATCTTCGTGGGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCT GGACAAGAACCAGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAG AACCAGCACCAGTACAAAGTGTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCA TCCTGCTCAACACCATCTGCCTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAA TATACTCAACATGCTTTTCACCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCC AAGGGTTACTTTAGTGATCCCTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCA TTCTCAGTGAGACTAATCCAGCTGAACATACCCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCG CATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGC ATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGA TGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGA GATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGA GAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCA ACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCT CTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTAGGGAT TGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCA AGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAA ATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACA GTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAG AGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGA CCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAA GAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCAC TGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGA TCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATC TTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTC CTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTC ACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCC AACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCA GCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAG CTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACC CGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCT CCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCC AAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGA CAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCAT TGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCC CACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGG GCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGA CAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGC TGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGA CTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACA TGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGG CACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGC TGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACC TGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001255998
Insert Size	6585 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001255998.2, NP_001242927.1
RefSeq Size	13415 bp
RefSeq ORF	6585 bp
Locus ID	12288
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 6, which is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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