Cacna1c (NM_001256000) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229706 representing NM_001256000 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATG ATGCCGTAGGAAGGGACTGGCCCTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACT TAACTTGGTTCTCGGTGTTCTTAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCC AGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGC ATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACT GTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATT CTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTC CTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAG CCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGC CTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTG GTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCA AGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGC CTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGG AAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTG TGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCC CTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTG AATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGG AGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGA GAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCC CCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCA ACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCT GTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCA AACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCT TCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATAT TCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTAAAGATGACTGCTTAC GGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCG TGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGT CCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCC ATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCC AGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAA CTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAG TTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAG AGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGAT CTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTC ATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAAT GTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGT GTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGC CTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCA CCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCC CTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAGACTAATAGT GCAGAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGC TGAGCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGT GGCTCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCC CTGAATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGT TCAGGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCC AGAGTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTC ATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTG ACTACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGA GTATGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCC CCACTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTC TGAACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAA AACAGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACT AGCATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATG CCACCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCC CTCACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGG CGGGCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTG CCTCCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGA CAGCAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAAC CAAGCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCAT CCACGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGG CTACTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAG GCAGCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGA TATTTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCT CTCCACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGG GAGATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATC TGGAATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCT GGTTCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACA TTCCCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTA CCCTACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTC CTGGTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTC ACCGTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGG TCTTGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCT GGCTGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCC CAGCAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGG CCCCAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAG GTCCTACGTCAGCAACCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256000
Insert Size	6462 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256000.2, NP_001242929.1
RefSeq Size	13634 bp
RefSeq ORF	6462 bp
Locus ID	12288
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (8) that is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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