Cacna1c (NM_001256000) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231917 representing NM_001256000 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATG ATGCCGTAGGAAGGGACTGGCCCTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACT TAACTTGGTTCTCGGTGTTCTTAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCC AGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGC ATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACT GTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATT CTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTC CTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAG CCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGC CTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTG GTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCA AGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGC CTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGG AAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTG TGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCC CTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTG AATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGG AGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGA GAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCC CCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCA ACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCT GTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCA AACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCT TCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATAT TCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTAAAGATGACTGCTTAC GGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCG TGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGT CCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCC ATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCC AGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAA CTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAG TTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAG AGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGAT CTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTC ATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAAT GTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGT GTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGC CTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCA CCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCC CTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAGACTAATAGT GCAGAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGC TGAGCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGT GGCTCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCC CTGAATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGT TCAGGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCC AGAGTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTC ATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTG ACTACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGA GTATGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCC CCACTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTC TGAACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAA AACAGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACT AGCATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATG CCACCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCC CTCACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGG CGGGCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTG CCTCCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGA CAGCAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAAC CAAGCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCAT CCACGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGG CTACTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAG GCAGCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGA TATTTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCT CTCCACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGG GAGATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATC TGGAATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCT GGTTCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACA TTCCCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTA CCCTACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTC CTGGTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTC ACCGTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGG TCTTGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCT GGCTGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCC CAGCAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGG CCCCAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAG GTCCTACGTCAGCAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231917 representing NM_001256000 Red=Cloning site Green=Tags(s) MVNENTRMYVPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGN ATISTVSSTQRKRQQYGKPKKQGGTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVA LAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSA ILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVII IYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFA FAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNRGAPAGLHDQKKGKFAWFSHSTETHVS MPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVF LNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETIL VETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGG KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILL NVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGES PPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSP NNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAY GAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVA IRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSK FDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFV IVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTIC LAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNS AEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIA LNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYF ISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQP PLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRT SMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIR RAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVTYYQSDSRGNFPQTFATQRPLHINKTGNN QADTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYSSTVSTVEGHGPPLSPAVRVQE AAWKLSSKRCHSRESQGATVNQEIFPDETRSVRMSEEAEYCSEPSLLSTDMFSYQEDEHRQLTCPEEDKR EIQPSPKRSFLRSASLGRRASFHLECLKRQKDQGGDISQKTALPLHLVHHQALAVAGLSPLLQRSHSPTT FPRPCPTPPVTPGSRGRPLRPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETTACSGSSSMARRARPVSL TVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMENAADNILSGGA QQSPNGTLLPFVNCRDPGQDRAVAPEDESCAYALGRGRSEEALADSRSYVSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256000
ORF Size	6459 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256000.2, NP_001242929.1
RefSeq Size	13634 bp
RefSeq ORF	6462 bp
Locus ID	12288
MW	242.3 kDa
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]

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