Tet1 (NM_001253857) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2510010B09Rik; AA517754; BB001228; Cxxc6; D10Ertd17e; LCX; mKIAA1676
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229698 representing NM_001253857 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGTCCCGCCCCGCAAAGCCTTCCAAATCAGTCAAAACAAAGCTACAGAAAAAGAAAGACATCC AGATGAAGACGAAGACATCAAAGCAGGCTGTCAGACATGGGGCATCAGCGAAGGCAGTGAACCCCGGAAA ACCGAAGCAATTAATTAAAAGAAGAGATGGTAAGAAGGAAACGGAAGACAAAACCCCGACACCGGCGCCG AGTTTTCTGACGCGAGCCGGGGCCGCCCGAATGAATCGGGATCGAAACCAAGTTCTTTTTCAGAACCCAG ACTCCTTAACTTGCAACGGGTTTACAATGGCTCTTCGAAGAACCTCTCTTAGCTGGCGACTCTCCCAGCG CCCAGTAGTCACACCCAAACCTAAGAAAGTACCACCTTCAAAGAAGCAGTGTACACATAATATCCAGGAC GAGCCGGGGGTAAAGCACTCTGAAAATGATTCAGTCCCCAGCCAACACGCTACCGTGTCCCCAGGCACAG AGAATGGTGAACAGAACAGGTGTTTAGTTGAAGGGGAGAGCCAAGAGATAACCCAGTCTTGCCCTGTATT TGAGGAAAGAATAGAAGATACCCAAAGCTGTATTTCCGCATCTGGGAACCTGGAAGCAGAAATTTCTTGG CCACTGGAAGGGACACATTGTGAAGAACTACTTTCTCATCAGACATCTGATAATGAGTGCACTTCCCCTC AAGAGTGTGCTCCATTGCCCCAAAGATCAACTTCCGAAGTTACCTCTCAGAAAAACACTAGCAATCAGTT AGCAGACTTGAGCTCACAAGTAGAGTCTATCAAGCTGTCTGATCCTTCTCCGAATCCTACGGGAAGCGAC CATAATGGCTTTCCAGACTCCAGTTTTAGGATCGTTCCTGAATTGGACCTGAAAACCTGCATGCCTCTTG ATGAGTCTGTGTATCCCACAGCTCTGATAAGATTCATCTTGGCAGGCTCACAGCCGGATGTCTTTGATAC TAAGCCCCAAGAAAAAACCCTTATAACAACCCCAGAACAAGTGGGAAGCCACCCAAATCAAGTTCTGGAT GCCACCTCTGTCCTAGGACAGGCCTTTAGTACTCTTCCACTTCAGTGGGGATTTTCTGGTGCTAACTTAG TTCAAGTTGAGGCCCTGGGCAAGGGTTCAGACTCACCAGAGGATCTTGGTGCTATTACTATGCTAAACCA ACAAGAAACTGTGGCTATGGATATGGATAGGAATGCTACCCCGGACCTGCCTATCTTCCTTCCTAAGCCT CCAAACACAGTTGCTACCTACAGTAGCCCTCTTTTAGGGCCTGAGCCCCACAGCTCTACCTCCTGTGGGC TTGAAGTCCAAGGTGCTACCCCGATTTTAACTTTGGACTCAGGACACACTCCCCAACTCCCACCAAACCC TGAGTCAAGTTCAGTACCTCTGGTAATAGCTGCAAACGGTACGCGAGCTGAGAAGCAGTTTGGTACCAGC CTTTTCCCAGCCGTCCCACAAGGGTTTACAGTCGCAGCCGAGAATGAAGTCCAGCATGCCCCATTAGATC TTACCCAGGGTTCTCAGGCTGCTCCCAGCAAACTGGAAGGAGAGATTTCTCGGGTCAGCATCACTGGCTC AGCTGATGTCAAAGCCACAGCGATGTCCATGCCGGTTACACAGGCCAGTACCTCTTCTCCCCCATGTAAT TCTACGCCGCCAATGGTGGAGAGGAGGAAGCGCAAGGCCTGTGGGGTCTGTGAGCCGTGCCAGCAGAAGG CCAACTGTGGGGAGTGCACCTACTGCAAGAATCGAAAGAACAGCCACCAGATCTGTAAGAAGAGGAAATG CGAGGTGCTCAAAAAGAAGCCAGAGGCCACCTCACAGGCACAGGTTACAAAAGAAAACAAGAGGCCCCAG AGGGAAAAGAAGCCCAAAGTTTTAAAGACAGACTTTAACAACAAACCAGTAAATGGCCCCAAATCAGAGT CCATGGACTGCAGTAGACGTGGTCATGGGGAAGAAGAGCAAAGGTTGGATTTGATCACACATCCCCTTGA AAATGTAAGGAAGAATGCAGGCGGCATGACGGGAATTGAGGTGGAGAAGTGGGCGCCAAACAAGAAATCA CACTTAGCAGAGGGTCAAGTCAAAGGAAGCTGTGATGCCAATTTAACTGGGGTGGAAAACCCCCAGCCCT CTGAAGATGACAAGCAGCAAACCAACCCTTCTCCGACATTTGCCCAGACCATAAGGAACGGCATGAAAAA TGTACACTGTTTACCCACTGACACACACCTTCCGCTCAACAAATTGAATCATGAGGAATTTAGCAAGGCG TTGGGAAATAATTCCTCTAAATTGCTGACTGATCCCTCAAACTGCAAGGATGCCATGAGTGTCACCACCT CCGGGGGGGAGTGTGATCATCTCAAGGGGCCAAGGAACACCCTGCTCTTCCAGAAGCCTGGCTTGAACTG CAGGTCTGGTGCAGAACCCACCATCTTTAATAATCACCCTAATACACACAGCGCTGGGAGTCGACCTCAC CCTCCTGAGAAGGTACCCAACAAAGAACCCAAAGATGGCTCTCCGGTTCAGCCAAGTCTTCTATCCCTAA TGAAGGACAGGAGATTAACGCTGGAACAAGTGGTAGCCATAGAAGCCCTGACTCAGCTCTCAGAAGCGCC CTCAGAGAGCTCCTCCCCATCCAAGCCGGAGAAGGATGAAGAAGCGCATCAGAAAACGGCTAGTCTGCTT AACAGTTGCAAGGCTATCCTTCATTCGGTGAGAAAAGACCTCCAAGACCCAAACGTTCAAGGAAAGGGCC TTCACCATGACACTGTTGTCTTTAATGGCCAGAACAGAACATTCAAGTCACCTGATAGTTTCGCTACTAA CCAAGCACTTATAAAATCACAAGGATATCCAAGTTCACCTACAGCTGAGAAGAAAGGCGCTGCTGGTGGG AGAGCTCCCTTTGATGGTTTTGAGAATTCACATCCATTGCCCATAGAAAGTCACAATCTTGAAAACTGCA GTCAGGTACTAAGCTGCGATCAGAATTTGAGTTCCCATGACCCATCATGCCAGGATGCACCCTACAGTCA AATTGAAGAAGATGTCGCAGCACAATTGACCCAACTTGCATCCACGATTAATCACATCAACGCCGAGGTC AGGAATGCTGAAAGTACACCAGAAAGCCTTGTTGCAAAGAACACAAAGCAAAAACACAGTCAGGAGAAAC GCATGGTACATCAGAAGCCACCCTCTAGTACACAAACTAAACCCAGTGTACCATCAGCAAAGCCAAAGAA GGCCCAGAAAAAGGCAAGAGCGACCCCACACGCAAACAAGCGGAAAAAGAAACCCCCAGCCCGAAGCTCT CAAGAAAATGATCAGAAGAAGCAAGAGCAGCTAGCTATAGAGTATAGTAAAATGCATGACATTTGGATGT CATCTAAGTTTCAAAGGTTTGGCCAGTCTAGTCCACGTAGTTTTCCTGTTCTGTTGAGAAATATTCCTGT CTTTAACCAAATATTAAAGCCCGTGACTCAGAGTAAAACACCCTCACAACATAATGAGTTATTTCCTCCT ATCAATCAGATAAAATTCACAAGAAACCCTGAATTGGCAAAGGAGAAAGTGAAGGTTGAACCATCAGATT CTCTGCCAACATGCCAATTCAAGACAGAATCCGGTGGGCAGACATTTGCAGAGCCGGCAGATAATTCTCA GGGACAGCCCATGGTGAGTGTCAATCAGGAAGCTCACCCTCTGCCCCAGTCCCCACCATCTAACCAGTGT GCTAATATAATGGCTGGTGCTGCCCAAACACAGTTTCATCTAGGCGCTCAAGAGAACCTGGTGCATCAGA TACCACCACCGACGTTGCCTGGTACCTCCCCTGACACACTCTTGCCAGACCCAGCATCAATCCTCAGGAA AGGGAAAGTGTTACATTTCGATGGAATTACAGTTGTTACGGAGAAGCGTGAAGCTCAAACATCAAGCAAT GGACCACTGGGTCCCACCACAGACAGTGCACAGTCAGAATTTAAAGAGAGCATCATGGACTTACTTAGCA AGCCTGCAAAAAACCTGATAGCTGGATTGAAGGAACAGGAAGCTGCACCCTGTGACTGTGATGGAGGTAC ACAAAAAGAAAAAGGCCCATATTATACACACCTTGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTC ATGGAGACTAGGTTTGGCCAGAAGGGGAAGGCAATCCGGATTGAGAAGATAGTGTTCACGGGGAAGGAAG GGAAGAGCTCTCAGGGCTGCCCGGTCGCCAAGTGGGTGATCAGAAGAAGTGGTCCTGAAGAGAAGCTTAT TTGTTTGGTTCGTGAGCGTGTAGACCATCACTGTTCGACGGCTGTGATAGTTGTCCTTATCCTGCTGTGG GAAGGTATCCCTCGCCTGATGGCTGACCGCCTGTACAAAGAGCTCACTGAGAACTTGAGGTCCTACAGCG GACATCCCACAGACCGAAGATGTACCCTCAACAAAAAGCGTACCTGCACCTGTCAAGGCATCGACCCAAA AACCTGCGGAGCGTCCTTCTCCTTTGGCTGTTCGTGGAGCATGTATTTCAACGGCTGTAAGTTTGGGAGG AGTGAAAACCCCAGAAAATTCAGACTTGCTCCAAACTACCCCTTACATAACTACTATAAGAGAATTACTG GAATGAGTTCTGAAGGAAGTGACGTGAAAACCGGGTGGATCATTCCAGACCGCAAGACCCTCATAAGCAG AGAGGAAAAACAGCTTGAAAAGAATTTACAAGAATTGGCTACAGTATTAGCTCCACTTTACAAGCAGATG GCTCCAGTTGCTTATCAAAATCAGGTGGAATATGAAGAAGTTGCTGGAGACTGTCGACTTGGAAATGAAG AGGGGCGTCCTTTCTCTGGTGTCACCTGTTGCATGGATTTTTGTGCCCATTCTCACAAGGACATTCACAA CATGCACAACGGAAGCACCGTGGTGTGTACGTTGATTCGAGCAGATGGCCGTGACACAAATTGTCCCGAG GATGAACAACTCCACGTCCTGCCACTATACCGGCTTGCAGACACTGATGAATTTGGCTCCGTGGAAGGGA TGAAGGCCAAAATCAAATCTGGGGCCATCCAAGTCAATGGGCCAACCAGGAAGAGGCGACTACGTTTTAC TGAGCCTGTTCCTCGATGTGGGAAGAGGGCCAAAATGAAGCAGAACCACAATAAATCAGGTTCACACAAC ACTAAGAGCTTTTCATCAGCCTCATCTACTTCTCACCTAGTGAAAGACGAATCTACAGACTTCTGTCCCC TGCAGGCTTCCTCCGCAGAAACATCTACCTGTACGTACAGTAAAACAGCCTCAGGTGGGTTTGCAGAAAC AAGTAGTATTCTCCACTGCACAATGCCTTCTGGAGCACACAGTGGTGCTAATGCAGCTGCTGGGGAATGT ACTGGAACGGTGCAGCCTGCCGAGGTGGCTGCTCATCCTCACCAGTCTCTTCCCACAGCCGATTCTCCCG TTCATGCTGAGCCTCTCACTAGTCCATCTGAGCAGCTAACTTCTAACCAGTCAAACCAGCAGCTCCCTCT CCTCAGCAATTCTCAGAAACTGGCTTCCTGTCAGGTGGAAGATGAGCGGCACCCTGAAGCGGATGAGCCT CAGCACCCCGAGGACGATAACTTGCCTCAACTTGATGAATTCTGGTCAGACAGTGAGGAGATCTACGCCG ATCCTTCCTTTGGTGGCGTGGCGATAGCACCCATTCACGGCTCGGTGCTCATTGAGTGCGCTCGGAAGGA GCTTCATGCTACCACCTCTTTGCGCTCCCCCAAACGAGGGGTCCCTTTTCGTGTGTCCCTTGTATTCTAC CAGCACAAAAGCCTAAACAAGCCTAATCATGGTTTTGATATCAACAAAATTAAGTGTAAATGCAAAAAAG TAACGAAAAAAAAGCCCGCAGACCGGGAGTGTCCTGATGTATCCCCCGAAGCCAATTTATCACACCAAAT TCCTTCTCGAGTTGCATCAACCTTAACCCGAGACAATGTTGTTACCGTGTCCCCATACTCTCTCACTCAT GTTGCGGGACCCTACAATCGTTGGGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253857
Insert Size	6120 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253857.1, NP_001240786.1
RefSeq Size	14082 bp
RefSeq ORF	6120 bp
Locus ID	52463
Gene Summary	Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.