Tet1 (NM_001253857) Mouse Untagged Clone

CAT#: MC229698

Tet1 (untagged) - Mouse tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1



  "NM_001253857" in other vectors (1)

CNY 19,760.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
TET1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2510010B09Rik; AA517754; BB001228; Cxxc6; D10Ertd17e; LCX; mKIAA1676
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229698 representing NM_001253857
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGGTCCCGCCCCGCAAAGCCTTCCAAATCAGTCAAAACAAAGCTACAGAAAAAGAAAGACATCC
AGATGAAGACGAAGACATCAAAGCAGGCTGTCAGACATGGGGCATCAGCGAAGGCAGTGAACCCCGGAAA
ACCGAAGCAATTAATTAAAAGAAGAGATGGTAAGAAGGAAACGGAAGACAAAACCCCGACACCGGCGCCG
AGTTTTCTGACGCGAGCCGGGGCCGCCCGAATGAATCGGGATCGAAACCAAGTTCTTTTTCAGAACCCAG
ACTCCTTAACTTGCAACGGGTTTACAATGGCTCTTCGAAGAACCTCTCTTAGCTGGCGACTCTCCCAGCG
CCCAGTAGTCACACCCAAACCTAAGAAAGTACCACCTTCAAAGAAGCAGTGTACACATAATATCCAGGAC
GAGCCGGGGGTAAAGCACTCTGAAAATGATTCAGTCCCCAGCCAACACGCTACCGTGTCCCCAGGCACAG
AGAATGGTGAACAGAACAGGTGTTTAGTTGAAGGGGAGAGCCAAGAGATAACCCAGTCTTGCCCTGTATT
TGAGGAAAGAATAGAAGATACCCAAAGCTGTATTTCCGCATCTGGGAACCTGGAAGCAGAAATTTCTTGG
CCACTGGAAGGGACACATTGTGAAGAACTACTTTCTCATCAGACATCTGATAATGAGTGCACTTCCCCTC
AAGAGTGTGCTCCATTGCCCCAAAGATCAACTTCCGAAGTTACCTCTCAGAAAAACACTAGCAATCAGTT
AGCAGACTTGAGCTCACAAGTAGAGTCTATCAAGCTGTCTGATCCTTCTCCGAATCCTACGGGAAGCGAC
CATAATGGCTTTCCAGACTCCAGTTTTAGGATCGTTCCTGAATTGGACCTGAAAACCTGCATGCCTCTTG
ATGAGTCTGTGTATCCCACAGCTCTGATAAGATTCATCTTGGCAGGCTCACAGCCGGATGTCTTTGATAC
TAAGCCCCAAGAAAAAACCCTTATAACAACCCCAGAACAAGTGGGAAGCCACCCAAATCAAGTTCTGGAT
GCCACCTCTGTCCTAGGACAGGCCTTTAGTACTCTTCCACTTCAGTGGGGATTTTCTGGTGCTAACTTAG
TTCAAGTTGAGGCCCTGGGCAAGGGTTCAGACTCACCAGAGGATCTTGGTGCTATTACTATGCTAAACCA
ACAAGAAACTGTGGCTATGGATATGGATAGGAATGCTACCCCGGACCTGCCTATCTTCCTTCCTAAGCCT
CCAAACACAGTTGCTACCTACAGTAGCCCTCTTTTAGGGCCTGAGCCCCACAGCTCTACCTCCTGTGGGC
TTGAAGTCCAAGGTGCTACCCCGATTTTAACTTTGGACTCAGGACACACTCCCCAACTCCCACCAAACCC
TGAGTCAAGTTCAGTACCTCTGGTAATAGCTGCAAACGGTACGCGAGCTGAGAAGCAGTTTGGTACCAGC
CTTTTCCCAGCCGTCCCACAAGGGTTTACAGTCGCAGCCGAGAATGAAGTCCAGCATGCCCCATTAGATC
TTACCCAGGGTTCTCAGGCTGCTCCCAGCAAACTGGAAGGAGAGATTTCTCGGGTCAGCATCACTGGCTC
AGCTGATGTCAAAGCCACAGCGATGTCCATGCCGGTTACACAGGCCAGTACCTCTTCTCCCCCATGTAAT
TCTACGCCGCCAATGGTGGAGAGGAGGAAGCGCAAGGCCTGTGGGGTCTGTGAGCCGTGCCAGCAGAAGG
CCAACTGTGGGGAGTGCACCTACTGCAAGAATCGAAAGAACAGCCACCAGATCTGTAAGAAGAGGAAATG
CGAGGTGCTCAAAAAGAAGCCAGAGGCCACCTCACAGGCACAGGTTACAAAAGAAAACAAGAGGCCCCAG
AGGGAAAAGAAGCCCAAAGTTTTAAAGACAGACTTTAACAACAAACCAGTAAATGGCCCCAAATCAGAGT
CCATGGACTGCAGTAGACGTGGTCATGGGGAAGAAGAGCAAAGGTTGGATTTGATCACACATCCCCTTGA
AAATGTAAGGAAGAATGCAGGCGGCATGACGGGAATTGAGGTGGAGAAGTGGGCGCCAAACAAGAAATCA
CACTTAGCAGAGGGTCAAGTCAAAGGAAGCTGTGATGCCAATTTAACTGGGGTGGAAAACCCCCAGCCCT
CTGAAGATGACAAGCAGCAAACCAACCCTTCTCCGACATTTGCCCAGACCATAAGGAACGGCATGAAAAA
TGTACACTGTTTACCCACTGACACACACCTTCCGCTCAACAAATTGAATCATGAGGAATTTAGCAAGGCG
TTGGGAAATAATTCCTCTAAATTGCTGACTGATCCCTCAAACTGCAAGGATGCCATGAGTGTCACCACCT
CCGGGGGGGAGTGTGATCATCTCAAGGGGCCAAGGAACACCCTGCTCTTCCAGAAGCCTGGCTTGAACTG
CAGGTCTGGTGCAGAACCCACCATCTTTAATAATCACCCTAATACACACAGCGCTGGGAGTCGACCTCAC
CCTCCTGAGAAGGTACCCAACAAAGAACCCAAAGATGGCTCTCCGGTTCAGCCAAGTCTTCTATCCCTAA
TGAAGGACAGGAGATTAACGCTGGAACAAGTGGTAGCCATAGAAGCCCTGACTCAGCTCTCAGAAGCGCC
CTCAGAGAGCTCCTCCCCATCCAAGCCGGAGAAGGATGAAGAAGCGCATCAGAAAACGGCTAGTCTGCTT
AACAGTTGCAAGGCTATCCTTCATTCGGTGAGAAAAGACCTCCAAGACCCAAACGTTCAAGGAAAGGGCC
TTCACCATGACACTGTTGTCTTTAATGGCCAGAACAGAACATTCAAGTCACCTGATAGTTTCGCTACTAA
CCAAGCACTTATAAAATCACAAGGATATCCAAGTTCACCTACAGCTGAGAAGAAAGGCGCTGCTGGTGGG
AGAGCTCCCTTTGATGGTTTTGAGAATTCACATCCATTGCCCATAGAAAGTCACAATCTTGAAAACTGCA
GTCAGGTACTAAGCTGCGATCAGAATTTGAGTTCCCATGACCCATCATGCCAGGATGCACCCTACAGTCA
AATTGAAGAAGATGTCGCAGCACAATTGACCCAACTTGCATCCACGATTAATCACATCAACGCCGAGGTC
AGGAATGCTGAAAGTACACCAGAAAGCCTTGTTGCAAAGAACACAAAGCAAAAACACAGTCAGGAGAAAC
GCATGGTACATCAGAAGCCACCCTCTAGTACACAAACTAAACCCAGTGTACCATCAGCAAAGCCAAAGAA
GGCCCAGAAAAAGGCAAGAGCGACCCCACACGCAAACAAGCGGAAAAAGAAACCCCCAGCCCGAAGCTCT
CAAGAAAATGATCAGAAGAAGCAAGAGCAGCTAGCTATAGAGTATAGTAAAATGCATGACATTTGGATGT
CATCTAAGTTTCAAAGGTTTGGCCAGTCTAGTCCACGTAGTTTTCCTGTTCTGTTGAGAAATATTCCTGT
CTTTAACCAAATATTAAAGCCCGTGACTCAGAGTAAAACACCCTCACAACATAATGAGTTATTTCCTCCT
ATCAATCAGATAAAATTCACAAGAAACCCTGAATTGGCAAAGGAGAAAGTGAAGGTTGAACCATCAGATT
CTCTGCCAACATGCCAATTCAAGACAGAATCCGGTGGGCAGACATTTGCAGAGCCGGCAGATAATTCTCA
GGGACAGCCCATGGTGAGTGTCAATCAGGAAGCTCACCCTCTGCCCCAGTCCCCACCATCTAACCAGTGT
GCTAATATAATGGCTGGTGCTGCCCAAACACAGTTTCATCTAGGCGCTCAAGAGAACCTGGTGCATCAGA
TACCACCACCGACGTTGCCTGGTACCTCCCCTGACACACTCTTGCCAGACCCAGCATCAATCCTCAGGAA
AGGGAAAGTGTTACATTTCGATGGAATTACAGTTGTTACGGAGAAGCGTGAAGCTCAAACATCAAGCAAT
GGACCACTGGGTCCCACCACAGACAGTGCACAGTCAGAATTTAAAGAGAGCATCATGGACTTACTTAGCA
AGCCTGCAAAAAACCTGATAGCTGGATTGAAGGAACAGGAAGCTGCACCCTGTGACTGTGATGGAGGTAC
ACAAAAAGAAAAAGGCCCATATTATACACACCTTGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTC
ATGGAGACTAGGTTTGGCCAGAAGGGGAAGGCAATCCGGATTGAGAAGATAGTGTTCACGGGGAAGGAAG
GGAAGAGCTCTCAGGGCTGCCCGGTCGCCAAGTGGGTGATCAGAAGAAGTGGTCCTGAAGAGAAGCTTAT
TTGTTTGGTTCGTGAGCGTGTAGACCATCACTGTTCGACGGCTGTGATAGTTGTCCTTATCCTGCTGTGG
GAAGGTATCCCTCGCCTGATGGCTGACCGCCTGTACAAAGAGCTCACTGAGAACTTGAGGTCCTACAGCG
GACATCCCACAGACCGAAGATGTACCCTCAACAAAAAGCGTACCTGCACCTGTCAAGGCATCGACCCAAA
AACCTGCGGAGCGTCCTTCTCCTTTGGCTGTTCGTGGAGCATGTATTTCAACGGCTGTAAGTTTGGGAGG
AGTGAAAACCCCAGAAAATTCAGACTTGCTCCAAACTACCCCTTACATAACTACTATAAGAGAATTACTG
GAATGAGTTCTGAAGGAAGTGACGTGAAAACCGGGTGGATCATTCCAGACCGCAAGACCCTCATAAGCAG
AGAGGAAAAACAGCTTGAAAAGAATTTACAAGAATTGGCTACAGTATTAGCTCCACTTTACAAGCAGATG
GCTCCAGTTGCTTATCAAAATCAGGTGGAATATGAAGAAGTTGCTGGAGACTGTCGACTTGGAAATGAAG
AGGGGCGTCCTTTCTCTGGTGTCACCTGTTGCATGGATTTTTGTGCCCATTCTCACAAGGACATTCACAA
CATGCACAACGGAAGCACCGTGGTGTGTACGTTGATTCGAGCAGATGGCCGTGACACAAATTGTCCCGAG
GATGAACAACTCCACGTCCTGCCACTATACCGGCTTGCAGACACTGATGAATTTGGCTCCGTGGAAGGGA
TGAAGGCCAAAATCAAATCTGGGGCCATCCAAGTCAATGGGCCAACCAGGAAGAGGCGACTACGTTTTAC
TGAGCCTGTTCCTCGATGTGGGAAGAGGGCCAAAATGAAGCAGAACCACAATAAATCAGGTTCACACAAC
ACTAAGAGCTTTTCATCAGCCTCATCTACTTCTCACCTAGTGAAAGACGAATCTACAGACTTCTGTCCCC
TGCAGGCTTCCTCCGCAGAAACATCTACCTGTACGTACAGTAAAACAGCCTCAGGTGGGTTTGCAGAAAC
AAGTAGTATTCTCCACTGCACAATGCCTTCTGGAGCACACAGTGGTGCTAATGCAGCTGCTGGGGAATGT
ACTGGAACGGTGCAGCCTGCCGAGGTGGCTGCTCATCCTCACCAGTCTCTTCCCACAGCCGATTCTCCCG
TTCATGCTGAGCCTCTCACTAGTCCATCTGAGCAGCTAACTTCTAACCAGTCAAACCAGCAGCTCCCTCT
CCTCAGCAATTCTCAGAAACTGGCTTCCTGTCAGGTGGAAGATGAGCGGCACCCTGAAGCGGATGAGCCT
CAGCACCCCGAGGACGATAACTTGCCTCAACTTGATGAATTCTGGTCAGACAGTGAGGAGATCTACGCCG
ATCCTTCCTTTGGTGGCGTGGCGATAGCACCCATTCACGGCTCGGTGCTCATTGAGTGCGCTCGGAAGGA
GCTTCATGCTACCACCTCTTTGCGCTCCCCCAAACGAGGGGTCCCTTTTCGTGTGTCCCTTGTATTCTAC
CAGCACAAAAGCCTAAACAAGCCTAATCATGGTTTTGATATCAACAAAATTAAGTGTAAATGCAAAAAAG
TAACGAAAAAAAAGCCCGCAGACCGGGAGTGTCCTGATGTATCCCCCGAAGCCAATTTATCACACCAAAT
TCCTTCTCGAGTTGCATCAACCTTAACCCGAGACAATGTTGTTACCGTGTCCCCATACTCTCTCACTCAT
GTTGCGGGACCCTACAATCGTTGGGTCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001253857
Insert Size 6120 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001253857.1, NP_001240786.1
RefSeq Size 14082 bp
RefSeq ORF 6120 bp
Locus ID 52463
Gene Summary Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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