Flna (NM_001290421) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ABP-280; Dilp2; F730004A14Rik; filamin-1; Fln1; GENA 379
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229690 representing NM_001290421 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACATCCGTGAGGCACCCCAGGATTTTCACCCAGACAGGGTGAAGGCACGTGGGCCTGGATTGG AGAAGACTGGTGTGGCTGTCAACAAGCCAGCAGAGTTCACAGTTGATGCCAAGCATGCTGGGAAGGCTCC TCTTCGAGTTCAAGTTCAGGACAATGAGGGCTGCTCTGTGGAAGCGACAGTCAAGGACAATGGCAATGGT ACTTACAGCTGTTCTTATGTGCCCAGAAAGCCAGTGAAGCACACAGCCATGGTTTCTTGGGGAGGTGTCA GCATCCCCAACAGTCCTTTCCGGGTGAATGTGGGAGCTGGCAGCCATCCAAACAAAGTCAAGGTGTATGG TCCAGGAGTGGCCAAGACTGGGCTCAAGGCCCATGAACCTACCTACTTTACTGTGGATTGTACTGAAGCT GGCCAGGGAGATGTCAGCATTGGTATCAAGTGTGCCCCTGGAGTAGTGGGCCCCACTGAGGCTGATATTG ACTTTGATATCATCCGCAATGACAATGACACCTTCACTGTAAAATACACACCCTGTGGGGCTGGCAGCTA TACCATCATGGTCCTTTTTGCTGACCAGGCCACACCCACCAGCCCCATCAGAGTCAAAGTGGAGCCTTCT CATGATGCCAGCAAGGTGAAGGCTGAGGGTCCTGGCCTAAATCGCACTGGTGTTGAACTTGGCAAACCCA CCCATTTCACAGTCAATGCTAAAACTGCTGGGAAAGGCAAGCTGGATGTCCAATTCTCAGGACTGGCTAA GGGAGATGCAGTACGGGATGTGGACATCATTGACCACCATGATAATACCTACACAGTCAAGTACATTCCT GTGCAGCAGGGCCCAGTAGGTGTCAATGTCACTTATGGAGGAGATCACATCCCCAAGAGTCCATTTTCAG TGGGAGTATCTCCAAGCCTGGATCTCAGCAAAATCAAGGTGTCTGGCCTTGGTGACAAAGTGGACGTTGG CAAAGATCAAGAGTTCACAGTAAAGTCAAAGGGTGCAGGTGGTCAAGGCAAAGTAGCATCCAAGATTGTG AGTCCCTCAGGTGCAGCGGTACCCTGCAAGGTAGAGCCAGGCCTGGGAGCTGACAACAGCGTGGTACGTT TTGTGCCCCGTGAAGAGGGGCCCTATGAGGTGGAAGTGACCTATGATGGTGTGCCTGTACCTGGCAGTCC CTTTCCACTAGAAGCTGTGGCCCCCACCAAACCCAGCAAGGTGAAGGCGTTTGGACCAGGGCTACAGGGG GGCAATGCAGGCTCCCCTGCCCGCTTCACCATTGATACAAAGGGTGCTGGCACTGGTGGCCTGGGCCTGA CAGTGGAAGGCCCCTGTGAAGCACAGCTTGAGTGCCTAGACAACGGGGATGGTACATGCTCTGTGTCTTA TGTACCCACTGAGCCTGGGGACTACAACATCAACATCCTTTTTGCTGACACCCACATTCCTGGATCCCCA TTCAAGGCCCATGTGGCTCCTTGTTTTGATGCATCCAAGGTGAAGTGCTCAGGCCCTGGGCTGGAGCGGG CTACTGCTGGTGAGGTAGGGCAGTTCCAAGTGGACTGTTCAAGTGCTGGCAGTGCTGAGTTGACGATTGA GATCTGCTCTGAGGCAGGACTGCCAGCTGAAGTATACATTCAAGACCATGGTGATGGCACACACACCATT ACCTATATTCCTCTCTGTCCTGGGGCTTACACTGTTACCATCAAGTATGGCGGCCAGCCTGTGCCCAACT TCCCCAGCAAGCTACAGGTGGAACCTGCTGTAGATACCTCAGGTGTACAGTGCTATGGGCCTGGGATTGA AGGTCAAGGTGTCTTCCGAGAGGCAACCACTGAGTTCAGTGTGGATGCCCGGGCTCTTACACAGACTGGA GGGCCACATGTCAAGGCTCGAGTGGCCAACCCCTCAGGCAATCTGACAGATACCTATGTGCAAGACTGTG GGGATGGCACATACAAAGTGGAATACACTCCATATGAGGAAGGAGTACACTCTGTGGATGTGACTTATGA TGGCAGCCCTGTGCCCAGCAGCCCCTTCCAGGTGCCTGTAACAGAGGGCTGTGACCCCTCCCGGGTGCGT GTCCATGGACCAGGCATCCAAAGTGGTACCACCAACAAACCCAACAAGTTCACAGTAGAAACCAGGGGAG CTGGCACAGGTGGCCTGGGCTTGGCTGTTGAGGGTCCCTCAGAGGCCAAGATGTCTTGTATGGATAATAA AGATGGCAGCTGCTCAGTAGAATACATCCCCTATGAAGCTGGAACCTATAGCCTTAATGTCACTTATGGT GGTCACCAAGTGCCAGGTAGTCCCTTCAAGGTCCCTGTACATGATGTGACAGATGCATCTAAAGTCAAGT GTTCTGGACCTGGCCTAAGCCCAGGCATGGTCCGTGCCAACCTCCCTCAGTCCTTTCAGGTGGACACAAG CAAAGCTGGAGTTGCCCCACTGCAGGTCAAAGTGCAGGGGCCCAAAGGCCTGGTGGAGCCAGTGGATGTA GTGGACAATGCTGATGGTACTCAGACTGTCAACTATGTGCCCAGCCGAGAAGGGTCCTATAGCATTTCTG TGCTGTATGGTGAAGAAGAAGTGCCACGGAGCCCCTTCAAGGTCAAGGTGCTGCCTACACATGATGCCAG TAAGGTGAAGGCCAGTGGACCTGGACTCAACACCACTGGTGTACCTGCTAGCCTGCCTGTGGAGTTCACC ATTGATGCCAAGGATGCTGGGGAGGGTCTGTTGGCTGTCCAGATTACGGATCCTGAAGGCAAGCCCAAGA AGACACACATTCAAGATAATCATGATGGCACATACACGGTGGCTTATGTGCCAGATGTGCCAGGCCGGTA CACAATCCTCATCAAGTATGGTGGTGATGAGATTCCCTTTTCCCCGTACCGTGTCCGGGCTGTGCCCACT GGGGATGCCAGCAAGTGCACAGTCACAGTGTCAATCGGAGGTCACGGGCTAGGTGCTGGCATTGGCCCCA CCATCCAGATTGGGGAGGAGACGGTGATTACTGTGGACACAAAAGCAGCAGGCAAAGGCAAAGTGACTTG TACTGTGTGCACACCTGATGGCTCAGAGGTAGACGTGGACGTGGTGGAGAATGAGGATGGCACCTTTGAC ATCTTCTACACAGCTCCCCAACCGGGCAAATATGTCATCTGTGTGCGCTTCGGTGGCGAGCATGTGCCCA ACAGCCCCTTCCAAGTTACAGCTTTGGCTGGGGACCAACCAACAGTGCAGACCCCATTAAGGTCTCAGCA GCTGGCTCCACAGTATAACTATCCTCAGGGTAGCCAGCAAACCTGGATTCCAGAGAGGCCCATGGTGGGC GTTAATGGGCTGGATGTGACCAGCCTGAGGCCCTTTGATCTTGTCATCCCCTTCACTATCAAGAAGGGGG AGATCACTGGGGAAGTTCGAATGCCCTCAGGCAAGGTGGCCCAGCCTTCCATTACTGATAACAAAGATGG CACTGTTACTGTACGTTACTCACCCAGTGAAGCTGGCCTGCATGAAATGGACATTCGCTATGACAATATG CATATCCCAGGAAGCCCTCTGCAGTTCTATGTTGATTATGTCAACTGTGGCCACATCACTGCTTATGGTC CTGGCCTTACCCATGGAGTGGTCAACAAACCTGCCACCTTCACTGTCAACACCAAGGATGCAGGAGAGGG GGGCTTGTCTCTGGCCATTGAGGGTCCATCTAAAGCAGAAATCAGTTGCACTGACAACCAGGATGGAACA TGCAGTGTCTCTTACCTGCCTGTACTGCCTGGTGACTATAGCATCCTAGTTAAGTACAATGATCAACACA TCCCAGGCAGTCCCTTTACTGCCAGAGTAACAGGTGACGATTCCATGCGTATGTCCCACCTAAAGGTGGG TTCTGCTGCTGATATCCCCATCAATATCTCAGAAACAGACCTTAGCCTACTCACAGCCACTGTGGTGCCA CCTTCGGGTCGAGAGGAACCCTGTCTGCTGAAACGTTTGCGAAATGGCCACGTGGGGATTTCCTTCGTGC CCAAGGAGACAGGGGAGCACCTGGTACATGTGAAGAAGAATGGCCAGCATGTGGCAAGCAGTCCCATCCC AGTAGTGATCAGCCAGTCGGAGATAGGTGATGCCAGCCGTGTGAGGGTCTCTGGTCAAGGTCTTCATGAA GGTCATACCTTTGAGCCTGCAGAGTTTATTATTGACACCAGAGATGCAGGCTACGGTGGGCTTAGTCTGT CCATTGAGGGCCCTAGCAAAGTAGACATCAACACAGAGGATCTGGAGGATGGCACATGCAGGGTCACCTA CTGTCCCACAGAGCCTGGAAACTACATTATAAACATCAAATTTGCTGACCAGCATGTGCCTGGCAGTCCC TTTTCTGTGAAGGTGACAGGTGAGGGCCGGGTGAAAGAGAGTATCACACGCAGGCGACGTGCCCCTTCTG TGGCCAATATTGGCAGTCATTGTGACCTCAGCCTGAAGATTCCTGAAATTAGCATCCAAGATATGACAGC CCAGGTGACCAGCCCATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGAACCATACTTACTGT ATCCGATTTGTGCCTGCTGAGATGGGAATGCATACAGTCAGTGTCAAGTACAAGGGCCAGCATGTACCTG GGAGCCCCTTCCAGTTCACTGTGGGGCCTCTGGGGGAAGGGGGTGCTCACAAGGTCCGTGCTGGAGGCCC TGGCCTAGAGAGGGCTGAAGTTGGAGTGCCAGCGGAGTTCGGCATTTGGACTAGGGAAGCTGGCGCTGGA GGCCTGGCCATTGCTGTTGAAGGCCCCAGCAAGGCTGAGATCTCTTTCGAGGACCGAAAGGATGGCTCCT GTGGTGTGGCCTACGTAGTTCAGGAGCCAGGTGACTATGAGGTCTCAGTCAAGTTCAACGAGGAGCACAT ACCTGATAGCCCCTTCGTGGTGCCTGTGGCTTCTCCGTCTGGTGACGCCCGCCGCCTTACTGTTTCTAGT CTTCAGGAGTCAGGGTTAAAGGTCAACCAGCCAGCATCTTTTGCAGTCAGTCTGAATGGAGCCAAGGGGG CAATTGATGCCAAGGTGCACAGCCCCTCAGGAGCTCTGGAGGAGTGCTATGTCACAGAGATTGACCAAGA TAAGTATGCTGTGCGTTTCATCCCACGAGAGAATGGCATCTACTTGATTGATGTCAAGTTCAATGGTACT CACATTCCTGGAAGTCCCTTCAAGATCCGAGTTGGGGAGCCTGGGCATGGAGGGGACCCAGGCTTAGTGT CCGCCTATGGAGCAGGCCTGGAAGGTGGTGTCACAGGGAGCCCAGCAGAGTTTATTGTGAACACAAGCAA TGCAGGAGCTGGTGCCCTTTCGGTTACCATTGATGGCCCCTCCAAGGTGAAGATGGATTGCCAGGAGTGC CCCGAGGGCTATCGTGTCACCTATACCCCCATGGCACCTGGCAGCTACCTCATCTCCATCAAGTATGGTG GCCCCTATCACATTGGTGGAAGTCCCTTTAAAGCCAAGGTCACAGGTCCTCGTCTTGTAAGCAACCACAG CCTCCATGAGACATCATCTGTGTTTGTGGACTCTTTGACTAAAGTTGCCACGGTTCCCCAGCATGCAACC TCAGGCCCAGGTCCTGCTGATGTCAGCAAGGTAGTAGCCAAAGGCCTGGGGCTAAGCAAGGCTTATGTAG GCCAGAAGAGCAACTTCACAGTAGATTGCAGCAAAGCAGGTAACAACATGCTGCTGGTGGGCGTGCATGG CCCAAGGACACCCTGTGAAGAGATCCTGGTGAAACACATGGGCAGCCGCCTCTATAGTGTCTCTTACCTG CTCAAGGACAAAGGGGAGTACACACTGGTGGTCAAGTGGGGTGATGAGCATATCCCAGGCAGCCCATACC GCATTATGGTACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290421
Insert Size	5967 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290421.1, NP_001277350.1
RefSeq Size	8369 bp
RefSeq ORF	5967 bp
Locus ID	192176
Gene Summary	Actin binding protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins (By similarity). Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs (PubMed:17172441). Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (PubMed:20713593).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains multiple differences compared to variant 1, including initiation of translation at a downstream in-frame start codon. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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