Mbd5 (NM_001290656) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430004D19Rik; AA536666; AI426407; C030040A15Rik; Gm1630; OTTMUSG00000012483
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229606 representing NM_001290656 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGGCAAAGAGTGTGACGGAGGAGACAAGGAAGGAGGTCTTGCAGCTATCCAGGTTCCTGTGG GTTGGCAGCGGCGTGTGGACCATAATGGCGTGCTGTACATCAGTCCCAGTGGGTCTTTGTTATCTTGCTT GGACCAGGTTAAGACATACCTGCTCACTGACGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTATTCTT CCCAAGGTGTTTAATTTTGATCCTGGAGCTGCTGTGAAGCAGAGAACTGCAGAAGATGTTAAGGCAGATG ATGATGTCACAAAGCTATGCATACATAAAAGAAAGATCATTGCAGTGGCCACACTCCATCAAAGCATGGA GGCCCCGCATCCTTCTCTGGTGCTCACCAGTCCTGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCA CGGGCGGCAACTCCAAGATCAGTAAGAAACAAGTCTCATGAAGGAATTACAAATTCTGTAATGCCAGAAT GTAAGAATCCTTTCAAGTTAATGACTGGATCATCAAATGCCATGGGAAGGCTATACATGCAAGACCTGCC TGGAAGCCAGCAGCAGGAGCTCCACCCTGTCTACCCCCGCCAGAGATTGGGCAGTAGTGAGCATGGACAG AAATCTCCATTTCGTGGCAGCCATGGAGGCCTGCCTAGCCCAGCATCATCAGGGTCTCAGATCTATGGAG ATGGCTCAATCTCTCCAAGGACTGACCCGCTCGGAAGCCCTGATGTCTTCACAAGAAATAACCCTGGTTT TCATGGAGCTCCCAATTCCAGCCCTATTCACCTAAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA CATGGTTCTCCCGTACAGTCATCCTGTGCAATGGCTGGAAGAACTAATATACCTCTTTCCCCAACCTTGA CTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAATATGGAAATACCACGAGCAAT GTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCTCCACCTTCTTGTGCTCTTCAGAAAAAGCCA TTAACATCTGAGAAAGATCCACTTGGCATTCTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTA TTATCATTAATCCAACCAGTTTCCATTCAAATGTCCACTCTCAGGTACCTGTGATGAATGTAAGCATGCC TCCTGCTGTTGTTCCTTTGCCAAGTAATCTCCCTTTGCCAACTGTAAAACCTGGTCATATGAATCATGGG AGTCATGTACAAAGAATTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACATCCCCAGTGC ACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCACGCTCATCTTCCACATCATC AGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAGGCCACTTGTAGTGGCATAAAGGTTCCACCT AGGTCACCAAGGTCAACAATAGGTTCCCCAAGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCTGATG GACATCATCAGTACAAGGATATCCCTAACCCATTAATTGCTGGAATGAGTAATGTACTCAATACCCCAAG CAGTGCAGCTTTTCCTACTGCACCTGCCGGAAATGGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTCTCAGCAGCAG CCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGCAGCAGTAACAATTCTGGAGC TGTTGCCAGCAGCGGCAACACTGAAGGACACAGCACTTTAAACACCATGTTCCCTCCAACTGCCAACATG CTTCTCCCAACAGGTGAAGGTCAAAGTGGTCGAGCAGCACTAAGAGATAAGTTGATGTCTCAGCAAAAAG ACTCATTACGGAAAAGAAAGCAGCCACCCACCACAGTGTTGAGTTTGCTCAGACAGTCCCAAATGGATAG TTCTGCAGCTCCTAAACCTGGACCTGACTTGCTAAGGAAGCATGGGCAGGGGTCATTTCCCATCAGTTCA ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACCCCGGGTTGTG GGGGATCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTCCAGATCCTAATATGAATTCCAC TGTTCTTCAGAATTCACTGACACAGAGCATACCTTTAAGAGGGGAAGCCGTGCACTGCCACAATGCAAAC ACTAACTTTGTTCACAGTAACAGTCCAGTCCCCAACCACCATCTTGCAGGTTTAATAAATCAGATTCAGG CTAGCGGGAACTGTGGGATGCTCAGTCAGTCGGGCATGGCTTTAGGAAACTCATTACATCCCAATCCACC TCAGTCAAGAATTTCAACGTCCTCCACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACA AGTTCTGAAGCAGGTGGTTCAAGCCTGCCGTCCTCCATAGCTATAGCTGGCAGCAACCACCCTGCCATCA CAAAGACAACATCTGTTCTCCAAGATGGTGTCATAGTCACCACCGCAGCTGGAAACCCACTGCAGAGTCA GCTGCCCATTGGGAGTGACTTTCCTTTTGTTGGCCAAGAGCATGCCCTTCATTTTCCATCCAACAGCACC GCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGCTCTCTACCTATCTCTTTGCCAGTGA ATCAACAGCATCTCCTAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAGGTGATATATC ATCCATAAACAATTCATTGAATAACCATCAACTGACTCATCTACAGTCGCTACTAAACAGCAATCAGATG TTTCCTCCAAATCAGCAGCCGCAGCAACACCTTCTCCAGGGACACCAGAATCTCCAGGCCTTCCAAGGAC AACCCACAGTCCCTTGCCCAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTCCAGGTGAGGAT GCAAGGAGATGCAGCTCTTCTAAATAAAAGAATAAGCACTCAGCCTGGGCTCACAACACTTCCTGAAAAC CCAAACCTTGCACTTCCACATTTCCAAGATACACCCTGTGAGCTGCAGCCAAGGATTGACCTTGGTCAGC CTATGAAGGACGGCCTCGTTATGGGTGGCCAAGGAGATGCCTCCGTAGACGCCATTTACAAAGCAGTTGT GGATGCAGCCAGCAAAGGAATGCAGGTTGTCATCACCACTGCTGTCAACAGCACGACTCAAATCAGCCCT ATCCCAGCCCTGAGCGCCATGAGTGCCTTCACTGCCTCCATTGGTGACCCATTAAATCTGTCCAGTGCTG TGAGCGCTGTCATCCACGGGCGGAACATGGGAGGAGTGGATCATGATGGCAGGCTGAGGAATGCGAGAGG GGCACGGCTACCCAAGAACATAGACCATGGGAAGAACTCCAGCGAAGGAGACGGGTTTGAGTGTTTCAAG TCAGCGAGCTGCCACACATCCAGAAAACAGTGGGATGGGGAGCAAAGTCCCAGAGGGGAGCGCAACAGGT GGAAGTATGAGGAGTTTTTAGATCATCCAGGCCATATCCACAGTAGTCCTTGCCACGAAAGGCCCAACAA TGTCTCTACACTGCCATTTCTAGCTGGGGAACAGCACCCGATACTGTTACCACCAAGAAACTGTCAAGGT GATAAGATTCTGGAGGAAAATTTCAGGTATAATAATTACAAAAGAACTATGATGAGTTTTAAAGAGAGAC TAGAGAGTACTGTGGAGAGATGTACACACATTAACGGAAACAGACCTCGTCAGAGTCGGGGCTTTGGAGA GCTGCTAGGCACTGCAAAACAAGACCTAGTTCTAGAGGGGCAGTCTCCGGGTTCTTCAAATAGTTTGGAA AGTTCTCTGGTCAAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAACCATTAATGGGTGTGTGC CTAGTCCCTCAGATGCTAAAAGCATTAGTAGTGAAGACGACCTACGGAATCCAGACTCCCCCTCCTCACA TGAGTTGATACACTATAGACCAAGGACGTTCAACGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTG ACTTCCTGGCCTGGGAAATTCATAAGAGAAGACGACGTTCACAATTCATGCCAACAAAGCCCCGAGGAAG GGAAGGTGGAGCCCGAGAAGTTGAAGACTCTCACAGAAGGTTTAGAAGCCTACAGCCGCGTCCGGAAAAG GAGCAGAAAAAGTGGGAAACTAAATAACCATTTAGAAGCTGCTATTCACGAGGCCATGAGCGAACTGGAC AAAATGTCTGGGACTGTTCACCAAATCCCACAGGGTGACAGACAAATGAGGCCCCCCAAACCCAAGAGGA GGAAGATCTCCAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290656
Insert Size	4497 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290656.1, NP_001277585.1
RefSeq Size	5301 bp
RefSeq ORF	4497 bp
Locus ID	109241
UniProt ID	B1AYB6
Gene Summary	Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. The 5' UTR of this variant is incomplete due to a lack of full-length transcript support and splice pattern ambiguity in the upstream region. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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