Ptprt (NM_001291151) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA0283; mRPTPrho; R-PTP-T; RPTP-rfo; RPTP-rho; RPTPmam4; RPTPrho
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229592 representing NM_001291151 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCTTGGCGGGCTCGCCCTCTGCCTGCTCCGGCTCCTGCTCCTGGGGCTGCAGCGGCCGCCGT TGCCCGGCGCCGGAGCGCAGAGCGCCGCAGGTGGCTGTTCTTTTGACGAACATTACAGCAACTGCGGGTA TAGCGTGGCTCTGGGAACCAATGGGTTTACCTGGGAGCAGATTAACACATGGGAGAAGCCAATGCTGGAC CCAGCTGTGCCCACAGGGTCCTTCATGATGGTGAACAGCTCTGGAAGGGCTTCAGGCCAGAAAGCCCATC TTCTTCTGCCAACCTTGAAGGAGAATGACACTCACTGCATTGACTTCCATTACTACTTCTCCAGTCGAGA TCGCTCCAGCCCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGACCCCAAGGGAACCCTGTCTGG AACGTATCTGGCGTCGTCACTGAGGGCTGGGTGAAGGCAGAGCTTGCCATCAGCACCTTCTGGCCTCATT TCTATCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTACATCGCTGTGGACGAAGTTCG GGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTGCGACTCCAAAACGTTGAGGTGAATGTGGGG CAGAACGCCACGTTTCAGTGCATTGCTGGTGGAAAGTGGTCCCAGCATGACAAACTTTGGCTCCAGCAAT GGAATGGCAGAGACACAGCCCTCATGGTCACCCGGGTGGTCAACCACAGACGCTTCTCAGCCACAGTGAG TGTGGCAGACACCTCTCAACGCAGTATCTCCAAGTATCGCTGCGTGATCCGCTCAGATGGTGGGTCTGGT GTGTCCAACTATGCAGAGCTGATTGTGAAAGAGCCTCCCACGCCCATTGCTCCCCCGGAACTACTGGCCG TGGGTGCCACCTACCTGTGGATTAAACCAAATGCCAACTCCATTATTGGGGATGGCCCCATCATACTGAA GGAGGTAGAATACCGCACAACCACAGGAACCTGGGCCGAGACCCACATCGTGGACTCTCCCAACTACAAG CTCTGGCATCTGGACCCTGATGTGGAGTATGAGATCCGGGTGCTGCTCACACGACCAGGAGAGGGGGGCA CAGGACCACCAGGACCACCCCTAACTACCAGGACCAAGTGTGCCGATCCCGTGCATGGCCCGCAGAATGT GGAGATTGTGGACATTCGGGCTCGGCAGCTGACCCTGCAGTGGGAACCCTTTGGCTATGCAGTGACCCGC TGCCACAGCTACAACCTCACAGTGCAGTACCAGTACGTGTTCAACCAGCAGCAGTATGAAGCTGAAGAGG TGATCCAGACATCTTCCCACTATACCCTTCGGGGTCTACGGCCCTTCATGACCATCAGACTGCGGCTGCT ACTGTCCAACCCTGAGGGCCGGATGGAGAGTGAGGAGTTGGTGGTACAGACAGAAGAGGATGTTCCAGGA GCTGTTCCTCTCGAGTCCATCCAAGGGGGTCCCTTTGAGGAGAAGATCTACATCCAGTGGAAACCTCCCA ATGAGACCAATGGGGTCATCACACTCTATGAGATTAACTACAAGGCTGTGGGCTCACTGGATCCAAGTGC TGACCTCTCCAGCCAGAGGGGAAAGGTGTTCAAACTCCGGAATGAAACCCACCACCTCTTTGTGGGTCTG TACCCTGGGACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCCCCAGTCACTA CTCGGATTGCCACCAAGATTTCAGCTCCATCAATGCCTGAATATGACGCAGATACCCCACTCAATGAAAC AGATACAACCATCACGGTGATGCTCAAACCCGCCCAGTCCAGGGGAGCCCCAGTCAGTGTTTACCAGCTG GTTGTCAAGGAGGAGCGACTCCAGAAGTCTCGGAGAGCAGCTGACATCATTGAGTGTTTCTCAGTACCTG TGAGCTACCGGAACGCCTCCAACCTCGATTCGCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAACCT GCCTGTCACCCAGCCATTTACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAACCCTCCTCTCTCC CCATTGAAGAGCTACAGCATCTACTTCCAGGCCCTCAGCAAAGCAAATGGAGAGACAAAAATCAACTGTG TGCGTCTGGCTACAAAAGGTGCTTCCACTCAGAATTCTAACACTGTGGAGCCGGAGAAGCAGGTCGACAA CACGGTGAAGATGGCTGGCGTGATTGCTGGCCTCCTCATGTTCATCATCATTCTCCTGGGGGTGATGCTG ACCATCAAAAGGAGAAGAAATGCTTATTCGTACTCCTATTACTTGAAGCTGGCTAAGAAGCAGAAGGAGA CCCAGAGTGGAGCCCAGAGGGAGATGGGTCCTGTGGCCTCGACTGACAAGCCTACCGCCAAGCTCGGCAC CAACCGCAATGATGAAGGCTTCTCCTCCAGCTCTCAGGATGTTAATGGATTCAATGGCAGCCGTGGGGAG CTGTCTCAGCCTACCCTCACCATTCAGACTCATCCCTACCGGACTTGTGACCCTGTAGAGATGAGCTATC CCCGGGACCAGTTCCAGCCTGCCATCCGGGTGGCGGACCTGCTTCAACACATCACCCAGATGAAGAGAGG CCAGGGCTACGGGTTCAAGGAGGAATACGAGGCCTTACCAGAAGGACAGACAGCTTCGTGGGACACAGCC AAGGAAGATGAAAACCGCAATAAGAATCGATACGGAAACATCATATCTTATGACCACTCTCGAGTAAGGC TGTTGGTGCTGGATGGAGACCCTCACTCAGACTACATCAATGCCAACTACATTGACGGGTACCACCGACC CCGGCACTACATTGCAACCCAAGGTCCAATGCAAGAGACGGTGAAGGACTTTTGGAGAATGATCTGGCAG GAAAACTCTGCCAGCATCGTCATGGTCACAAACCTTGTGGAAGTGGGCAGGGTGAAGTGTGTCCGATACT GGCCAGATGACACAGAGGTCTATGGAGACATTAAAGTCACCCTAATAGAAACAGAGCCCCTGGCAGAATA CGTCATCCGCACCTTCACAGTCCAGAAGAAAGGCTACCATGAGATCCGGGAGCTCCGCCTCTTCCACTTC ACCAGCTGGCCTGACCACGGTGTTCCCTGCTATGCCACTGGCCTTCTGGGCTTTGTCCGCCAGGTCAAGT TTCTCAATCCCCCAGAAGCTGGGCCCATAGTGGTCCATTGCAGTGCTGGAGCCGGGAGGACTGGCTGCTT CATTGCGATTGACACCATGCTCGACATGGCTGAGAATGAAGGGGTTGTGGACATCTTCAACTGTGTGCGT GAGCTCCGGGCACAGAGGGTCAACCTGGTGCAGACAGAGGAGCAGTACGTGTTTGTGCACGATGCCATCC TGGAAGCATGCCTCTGCGGCAATACTGCCATCCCAGTGTGTGAGTTCCGCTCTCTCTACTACAACATCAG CAGGCTGGACCCGCAGACCAACTCCAGCCAGATCAAAGACGAGTTTCAGACACTCAACATTGTGACACCT CGAGTGCGGCCTGAAGACTGCAGCATTGGGCTCTTACCCCGGAACCATGATAAGAATCGGAGCATGGATG TCCTGCCTCTGGACCGCTGTCTACCCTTCCTCATCTCAGTAGATGGAGAGTCCAGCAACTACATCAATGC AGCACTGATGGATAGCCACAAGCAGCCTGCCGCCTTCGTGGTCACCCAGCATCCTCTACCCAACACGGTG GCAGACTTCTGGAGGCTGGTGTTTGATTATAATTGTTCATCTGTGGTGATGCTGAACGAGATGGATACTG CTCAGCTCTGTATGCAGTACTGGCCTGAGAAGACCTCCGGGTGTTATGGTCCCATCCAGGTGGAGTTTGT CTCTGCAGACATCGATGAGGACATCATCCACAGAATCTTCCGGATCTGTAACATGGCTCGGCCACAGGAT GGTTATCGTATTGTCCAGCACCTCCAGTACATCGGCTGGCCTGCATACCGGGACACGCCCCCCTCTAAGC GCTCTCTGCTCAAAGTGGTCCGACGGCTGGAGAAATGGCAGGAGCAATACGACGGAAGAGAGGGGCGCAC TGTGGTCCACTGCCTAAATGGGGGAGGCCGCAGTGGAACCTTCTGTGCTATCTGCAGTGTGTGTGAGATG ATCCAGCAGCAGAACATTATTGACGTGTTCCACATTGTGAAAACCCTCCGCAACAACAAGTCCAACATGG TGGAGACGCTGGAACAGTATAAATTTGTATATGAGGTGGCACTGGAATATTTAAGCTCCTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291151
Insert Size	4335 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291151.1, NP_001278080.1
RefSeq Size	12126 bp
RefSeq ORF	4335 bp
Locus ID	19281
Gene Summary	May be involved in both signal transduction and cellular adhesion in the CNS. May have specific signaling roles in the tyrosine phosphorylation/dephosphorylation pathway in the anterior compartment of the adult cerebellar cortex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site and contains an additional in-frame exon but lacks a different in-frame exon in the central coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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