Spag9 (NM_001199204) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110018C07Rik; 4733401I23Rik; 4831406C20Rik; AW552012; Jip4; JLP; JSAP2; JSAP2a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229557 representing NM_001199204 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGAGGACGGTGTGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGATGTCAGAGCGGG TGTCCGGCCTGGCCGGCTCCATCTACCGCGAGTTCGAGCGGCTCATCGGGCGCTACGATGAGGAGGTGGT CAAGGAGCTGATGCCACTGGTGGTGGCCGTGCTGGAGAACCTGGACTCGGTGTTCGCGCAGGACCAGGAG CACCAGGTGGAGCTGGAGCTGCTGCGGGACGACAACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGG CGCTGCGCAAGCACGCCGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAAGGATTT ACAGACGCGAGTGGAGTCTCTAGAATCTCAAACAAGACAACTTGAACTCAAAGCAAAAAACTATGCTGAC CAGATCAGCAGACTTGAAGAGAGAGAAGCAGAACTGAAGAAGGAATATAATGCACTGCATCAGAGACACA CTGAGATGATCCATAATTATATGGAACACTTAGAAAGAACCAAACTTCATCAGCTCTCAGGGAGTGATCA ACTAGAGGCTACAGCTCATAGTAGAATTAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCT GCTGGAGATGGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAAT TTCAAGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGG ATCTAAAGCTACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCA AAGGAAGATAATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAG AAGTCCAGGTTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGT TCAAGCAATCATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGC ACTCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCT CAGCTGGCTCGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGA GAATCTCATACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTA ATAGCCAAAGTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAG CCAAGCTGAAGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGA AGATGCAAGGCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACC AGAGTAGAAATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAG CTGTTCGATGGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAG CATCTGGCAATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTT AACCTGAAGTACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTC AGCTCCCTGGGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCG CAGAGAGCAAAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAA GCTTTCGGCTGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAA ATTTACCTGTGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGT AGGAGTCAATTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAG GATATTGCTGGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGG ATCAAGAACTCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTG TACCAGCACCCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGT TTCACTGTTTGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTG CAGGAGAAGAACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAG CTCCGCAGAGATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGA GCTGCCACTTCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAA ATAGCGAAGTTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTC CACTGAAGACACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGA GTTCAGATCCCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAA TATCAGTATTGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAAC CATGTGGCTTGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTC CATTCCATTAAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGG CGGATGGCACCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTT AGACCTTGGACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTAT AGGAACAAAATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGA AGGAGAGCCAAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCAC GCTCCGCCTCTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAA ATGTTAGGTACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTT TGTGGGTGGGGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACCGTAATCCTCCACCAGGG ACGTTTACTGGGGCTAAGGGCAAATAAAACCTCAGGGACTCCAGGTAACCGTCCTGGAAGTGTAATCCGT GTATATGGTGATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATACCCTACTGTTCAATGGCACATG CACAGCTTTGTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAGTCCCAGGTCAGGTCATTAG CCCACAAAGTAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGGGTCATCTGCACAGGAGCCCAGT AGCCAGACGCCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGCTACATTGACTTCCGGATGGGTG ATGAAGGTGGAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAACCTTCAGTCACCAAAGCCGAGAG GAGTCACCTGATAGTGTGGCAAGTGATGTGTGGCAATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199204
Insert Size	3963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199204.1, NP_001186133.1
RefSeq Size	7389 bp
RefSeq ORF	3963 bp
Locus ID	70834
UniProt ID	Q58A65
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) has multiple differences in the coding region, compared to variant 5, which results in a shorter isoform (6), compared to isoform 5.

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