Arhgap33 (NM_001289670) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW324378; NOMA-GAP; Snx26; TCGAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229541 representing NM_001289670 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCCGCAGCACTGACAGCCTGGATGGCCCAGGGGAGGGCTCAGTGCAGCCCGTACCCACTACTG GGGGGCCGGGTACAAAGGGGAAGCCTGGGAAGAGGCTCTCAGCTCCTCGAGGCCCTTTCCCCCGGCTGGC TGACTGTGCCCATTTCCACTATGAGAATGTTGACTTTGGCCACATTCAGCTCCTGCTGTCTCCAGAGCGT GAGGGCCCCAGCCTCTCTGGAGAAAATGAGCTGGTCTTTGGGGTACAGGTGACCTGTCAGGGCCGCTCCT GGCCAGTCCTCCGGAGCTATGACGATTTCCGTTCTCTGGATGCCCATCTCCACCGGTGCATATTTGACCG GAGATTTTCCTGCCTCCCAGAGCTCCCTCCACCTCCAGAGGGTACCAGGGCTGCCCAGATGCTGGTACCG CTGCTGCTGCAGTACCTGGAGACCCTGTCAGGACTGGTGGACAGTAACCTCAACTGTGGACCTGTGCTCA CTTGGATGGAGCTGGACAACCATGGCCGGCGCCTGCTCCTCAGTGAGGAAGCCTCCCTCAATATCCCTGC TGTGGCTGCTGCCCACGTGGTCAAGCGGTACACTGCCCAGGCGCCAGATGAGCTGTCCTTTGAGGTGGGA GACATTGTCTCAGTGATCGACATGCCACCTACAGAGGATCGGAGCTGGTGGCGGGGCAAACGGGGCTTCC AGGTTGGTTTCTTCCCCAGTGAGTGTGTGGAACTCTTCACGGAGAGGCCAGGGCCTGGCCTAAAGGCAGA TGCTGACAGTCCCCTGTGTGGCATCCCAGCTCCCCAGGGCATCTCTTCTCTGACCTCAGCTGTGCCCCGG CCACGTGGGAAGCTGGCAGGACTCCTCCGCACCTTCATGCGCTCTCGTCCTTCTCGCCAGCGGCTGCGCC AACGGGGAATCCTGCGGCAGAGGGTGTTTGGTTGTGATCTTGGAGAGCACCTCAGCAACTCAGGCCAAGA CGTGCCCCAAGTGCTGCGCTGCTGCTCTGAGTTTATTGAGGCCCATGGTGTGGTGGATGGAATCTACAGG CTCTCTGGCGTGTCCTCCAACATCCAGAGGCTTCGGCATGAGTTTGATAGTGAGAGGATCCCTGAGCTGT CTGGCCCCGCCTTCCTACAAGATATCCACAGTGTGTCCTCCCTCTGCAAGCTCTACTTCCGGGAACTGCC CAACCCCTTGCTCACCTACCAGCTCTATGGGAAGTTCAGTGAGGCTATGTCAGTGCCTGGGGAGGAAGAA CGCCTAGTGCGAGTGCATGACGTCATCCAGCAGCTGCCTCCACCACACTACAGGACCCTGGAGTACCTGC TGAGGCACCTGGCCCGCATGGCAAGACACAGCGCTAACACCAGCATGCATGCCCGCAACCTGGCCATTGT CTGGGCACCCAACCTGCTACGGTCTATGGAACTGGAGTCGGTGGGGCTGGGTGGGGCAGCCGCCTTCCGG GAGGTCCGGGTGCAGTCGGTGGTGGTGGAGTTCCTGCTTACCCACGTGGAGGTTCTGTTCAGCGATACGT TCACCTCTGCTGGCCTGGACCCTGCAGGTCGCTGCCTCCTTCCCAGACCCAAGTCCCTTGCGGGGAGCAG TCCCTCCACTCGCCTGCTAACACTGGAAGAAGCCCAGGCACGAACTCAGGGCCGTCTTGGAACACCTACA GAGCCCACGACTCCTAAGACGCCAGCCTCACCAGTGGAAAGGAGGAAAAGAGAGAGGGCGGAGAAACAAA GGAAGCCAGGAGGTAGCAGCTGGAAGACATTCTTTGCTCTGGGGCGGGGCCCCAGCATACCCCGGAAGAA GCCGCTGCCATGGCTGGGTGGCAGCCGAGCCCCACCACAGCCTTCAGGCAGCAGACCTGACACTGTCACG CTGAGATCTGCCAAAAGTGAGGAGTCTCTGTCATCCCAGGCCAGCGGGGCTGGCCTCCAGAGGCTGCACC GGCTGCGACGACCCCACTCCAGCAGCGATGCTTTTCCCGTGGGCCCAGCACCTGCTGGCTCTTGCGAGAG CTTGTCCTCTTCTTCTTCCTCTTCTTCCTCTTCCTCCTCCTCCTCCTCCTCAGAGTCTTCAGCAGGTGGC CTGGGGCCACTCTCTGGCTCCCCCTCACACCGCACCTCAGCCTGGCTAGATGATGGCGATGATCTGGATT TCAGTCCACCCCGCTGTCTGGAAGGACTTCGGGGACTTGACTTTGATCCCCTTACCTTTCGCTGCAGCAG CCCCACCCCAGGGGACCCTGCACCTCCTGCCAGCCCAGCACCTCCAGCCTCAGCCTCTGCCTTCCCACCT CGGGCAACCCCACAGGCTCTGTCACCCCATGGACCCACTAAGCCTGCTTCACCCACTGCCCTGGACATCT CAGAGCCCCTGGCTGTATCAGTACCACCTGCTGTCCTGGAACTACTGGGAGCTGGAGGAACGCCTGCCTC AGCCACCCCAACACCGGCTCTCAGCCCTCATCTCATCCCCCTGCTGCTGCGTGGAGCTGAGGCCCAGCTA AGTGACACCTGTCAGCAGGAGATCAGCAGCAAGCTAGCACCAACCCGGGGAGCTCCAGGCCAGCAAAGTC CTGGCGGCATGGATTCACCGTTATTGCCCCCACCCTTGCCTCTGCTGCGCCCTGGTGGGGCTCCGCCCCC TCCCCCCAAGAACCCAGCTCGTCTCATGGCCCTGGCCTTGGCTGAGCGAGCTCAGCAGGTAGCAGAGCAA CAGAGCCAACAAGAGCAGGGGGGCACCCCACCTGCTCCCCACTCCCCCTTTCGACGCTCTCTGTCCCTAG AGGTGGGTGGGGAGCCTGTGGGGACTTCAGGGAGTGGGATACACCCTCCCTCCTTAGCTCACCCAGGTGC CTGGGCTCCAGGTCCCCCACCTTACCTCCCAAGGCAACAGAGTGATGGGAGCCTGGTAAGAAGCCAGCGG CCCTTGGGGACCTCAAGGAGAAGTCCCAGAGGGCCTTCCCAGGTCAGTGCCCATCTCAGGGCAAGCGGGG CTTACAGGGATGCTCCAGAGATGGCAGCCCAGTCACCATGTTCTGTCCCCTCACAGGGTTCTAACCCCAG CTTCTTCTCAACCCCCCGAGAGTGTTTGCCACCTTTCCTTGGCGTCCCCAAACAAGGCTTGTACTCTCTG GGACCCCCATCCTTCCCACCTAGCTCTCCAGCCCCAGTCTGGAGGAACTCTCTGGGTGCCCCCTCAGCAT TGGACAGGGGAGAGAATCTGTACTATGAAATTGGGGTAGGGGAGGGGACCTCCTACTCAGGCCCCAGCCG GTCCTGGAGTCCGTTTCGCTCCATGCCTCCCGACAGGCATAATGCCTCCTATGGTATGCTTGGCCAGTCA CCACCGCTCCACAGGTCCCCTGATTTCCTGCTCAGCTACCCGCCTCCCCCCTCCTGCTTTCCCCCAGAGC ACCTTACCCACTCAGTTTCACAGCGCCTTGCCCGGCGCCCTACCCGTCCTGAGCCCCTCTATGTCAACCT AGCCCTAGGGCCCAGGGGCCCTTCACCTGCCTCTTCTTCCTCTTCCTCTCCTCCTGCCCACCCCAGGAGC CGGTCAGATCCTGGGCCTCCTGTTCCCCGCCTCCCTCAGAAACAGCGGGCTCCTTGGGGTCCACATACTC CCCATCGGGTGCCTGGACCTTGGGGTTCCCCCGAACCTTTTCTGCTCTATAGGCCAGCTCCACCATCCTA TGGGAGGGGAGGGGAGGTCCGAGGATCCTTGTACAGAAATGGAGGACATAGAGGAGAGGGGGCTGGTCCC CCTCCTCCTTACCCCACACCCAGCTGGTCCTTACATTCAGAAGGACAGACCAGAAGTTACTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289670
Insert Size	3846 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289670.1, NP_001276599.1
RefSeq Size	4356 bp
RefSeq ORF	3846 bp
Locus ID	233071
UniProt ID	Q80YF9
Gene Summary	May be involved in several stages of intracellular trafficking (By similarity). Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon that includes a portion of the 5' UTR and 5' coding region, resulting in translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a.

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