Kcnma1 (NM_001253358) Mouse Untagged Clone

CAT#: MC229526

Kcnma1 (untagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 1



  "NM_001253358" in other vectors (1)

CNY 12,160.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal Anti-KCa1.1 (1184-1200)
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229526 representing NM_001253358
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT
ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG
ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT
CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC
AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA
TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC
TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA
CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC
TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC
ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG
TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC
TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT
GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC
ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA
GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA
ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACAT
CCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCC
CAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGA
TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTC
CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATG
ATAGCCATTGAGTACAAGTCTGCCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGA
TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG
CAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTGATCTAT
TTTGAAGATGAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGC
CCAACACCTCCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACAT
GGACTCCAATGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAA
GTCATCTTGACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGG
ATGTCAGCTCAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCA
TGAGCTCAAACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAAC
TTCCCGAAAGTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACC
TCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGA
ATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAAT
TCCCAAGGATTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTAC
GCCAGCCGTCCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTCGCTAAGCCGGGCAAGTTGCC
TTTGGTATCAGTCAATCAGGAAAAAAACAGTGGGACGCACATTCTAATGATAACGGAGTTGGTGAATGAT
ACCAATGTTCAGTTTTTGGACCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCACACAGCCCT
TTGCTTGTGGGACAGCATTTGCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTTCAATGACAA
TATCCTCACCCTAATACGGACCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAG
GAGAATGCACTTCGAGGAGGCTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCC
AGTTAGCCCTGTTAGATGGTCCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAA
AGCTCTGAAAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCC
AGCCAGTGTACAAAAAGGTACGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGA
TCTTCTGCCTGATGCAGTTTGACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTC
CTCACAGTCGTCCAGTAAGAAGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGG
CCCAAGTCCAGGGAGTCCCGCGACAAACAGAATGCAACAAGGATGACTAGAATGGGCCAAGCAGAAAAGA
AATGGTTTACAGATGAGCCGGATAATGCCTATCCCAGAAACATTCAAATCAAGCCCATGAGTACCCACAT
GGCTAACCAGATCAACCAATATAAATCCACAAGCAGCCTGATCCCTCCAATCAGAGAAGTTGAAGATGAA
TGTTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001253358
Insert Size 3786 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001253358.1, NP_001240287.1
RefSeq Size 5063 bp
RefSeq ORF 3786 bp
Locus ID 16531
Gene Summary Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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