Cyfip2 (NM_001252460) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1500004I01Rik; 6430511D02Rik; AA930218; AU022376; mKIAA1168; Pir121
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229520 representing NM_001252460 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCCACGTCACTTTGGAAGATGCCCTGTCCAACGTGGACCTGCTGGAGGAACTGCCCCTCCCAG ACCAGCAGCCATGTATCGAGCCCCCGCCATCCTCCATCATGTACCAGGCTAACTTTGACACGAACTTCGA GGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATTGAGCAGGCAACGGTCCACTCCAGCATGAAT GAAATGCTGGAGGAGGGCCACGACTATGCAGTCATGCTGTACACCTGGCGCAGCTGCTCCCGGGCCATCC CGCAGGTGAAGTGCAATGAGCAGCCCAATCGGGTCGAGATCTACGAGAAGACGGTGGAGGTGCTGGAGCC AGAGGTCACCAAGCTCATGAAGTTCATGTACTTTCAGCGCAAGGCCATCGAGCGCTTCTGTAGCGAGGTG AAGCGGCTGTGTCACGCTGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTGCTGACGCTGGGCAAGT TCATCAACATGTTCGCGGTCTTGGACGAGCTAAAGAACATGAAGTGCAGTGTCAAGAATGACCACTCTGC CTACAAGAGAGCAGCACAGTTCCTAAGGAAGATGGCGGACCCCCAGTCCATCCAGGAATCTCAGAACCTG TCCATGTTCCTGGCCAATCACAACAGGATCACCCAGTGTCTTCACCAGCAACTGGAAGTGATCCCGGGCT ATGAGGAGCTGCTGGCAGACATTGTCAACATCTGTGTTGATTACTATGAGAATAAGATGTACCTGACGCC CAGTGAAAAGCACATGTTGCTCAAGGTGATGGGCTTTGGTCTCTATCTGATGGACGGAAATGTCAGTAAC ATTTACAAACTGGATGCCAAGAAGAGAATCAACCTTAGCAAAATTGATAAGTTTTTTAAGCAGCTGCAGG TGGTACCCCTTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAGACCAGTGCTCACTATGAAGA GAACAAGTCCAAGTGGACATGCACTCAGAGCAGCATCAGCCCCCAGTACAACATCTGTGAGCAGATGGTT CAGATCCGGGATGATCACATCCGCTTCATCTCTGAGCTGGCTCGCTACAGCAACAGCGAGGTGGTGACAG GCTCAGGGCTGGACAGCCAGAAGTCGGATGAAGAGTACCGGGAGCTTTTTGACCTGGCCCTGCGAGGCCT GCAGCTTCTATCCAAGTGGAGTGCCCATGTCATGGAGGTGTACTCTTGGAAGCTGGTTCACCCCACAGAC AAGTTCTGCAACAAGGACTGCCCGGGCACCGCGGAAGAGTACGAGAGAGCCACGCGCTACAATTATACCA GCGAGGAGAAGTTTGCCTTTGTGGAGGTGATTGCCATGATCAAGGGTTTGCAGGTGCTCATGGGAAGGAT GGAGAGTGTCTTCAACCAGGCCATCCGCAACACTATCTACGCAGCCCTGCAGGATTTCGCCCAGGTGACG CTGCGGGAGCCGTTGCGGCAAGCAGTTCGAAAGAAAAAGAATGTCCTTATCAGCGTTCTGCAAGCAATTC GAAAGACCATCTGTGACTGGGAGGGAGGCCGAGAGCCCCCCAATGACCCATGCTTAAGAGGGGAGAAGGA CCCCAAAGGTGGCTTTGACATCAAGGTGCCCCGCCGTGCTGTGGGTCCATCCAGCACACAGCTGTACATG GTGCGCACCATGCTTGAGTCACTCATCGCAGACAAGAGCGGCTCCAAGAAGACGCTCAGAAGCAGCCTGG ATGGCCCTATCGTCCTTGCCATAGAGGACTTCCACAAGCAGTCCTTCTTTTTCACACACCTGCTTAACAT CAGCGAGGCTCTTCAGCAGTGCTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGTTGACC ATGGGCCGGCGAATCCAGTTCCCGATTGAGATGTCCATGCCCTGGATTCTAACGGACCATATTCTGGAAA CCAAAGAACCTTCCATGATGGAGTATGTCCTCTACCCCTTGGATCTGTACAACGACAGCGCCTACTATGC CCTCACCAAATTCAAAAAGCAGTTCCTGTATGACGAGATCGAAGCCGAGGTAAACCTGTGTTTTGATCAG TTTGTCTACAAGCTGGCTGACCAGATCTTCGCCTACTACAAAGCCATGGCCGGCAGTGTCCTGCTGGATA AACGTTTTAGAGCTGAGTGTAAGAACTATGGGGTGATCATACCGTACCCGCCATCCAACCGCTACGAGAC ACTGCTGAAGCAGAGACATGTCCAGCTGTTGGGGAGATCAATTGACTTGAACAGACTCATCACCCAGCGG ATCTCTGCTGCCATGTACAAGTCCTTGGACCAGGCCATCAGCCGCTTTGAGAGTGAAGACCTGACCTCCA TTGTGGAGCTGGAGTGGCTGCTGGAGATTAACCGACTCACACACCGACTCCTCTGTAAGCACATGACGCT AGACAGCTTCGATGCCATGTTCCGAGAAGCCAATCATAACGTGTCTGCCCCTTACGGCCGCATCACCCTG CATGTCTTCTGGGAGCTGAACTTTGACTTTCTCCCCAACTACTGCTATAATGGATCCACAAACCGTTTTG TCCGAACTGCCATCCCTTTCACCCAAGAACCACAACGGGATAAGCCCGCCAATGTCCAGCCTTATTACCT CTATGGATCCAAGCCTCTCAACATTGCCTACAGCCACATCTATAGCTCCTACCGGAACTTTGTGGGGCCC CCTCACTTCAAGACCATCTGCAGACTGTTGGGCTACCAGGGCATCGCTGTGGTCATGGAGGAACTGCTGA AAATCGTCAAGAGCTTGCTCCAAGGCACCATCCTGCAGTACGTGAAGACGCTCATAGAGGTGATGCCGAA AATCTGTCGTTTGCCTCGGCACGAGTACGGCTCTCCAGGAATCCTGGAGTTCTTCCACCACCAACTGAAG GACATCATCGAGTATGCAGAACTCAAAACAGACGTGTTCCAGAGCCTCCGAGAGGTGGGCAACGCCATCC TGTTCTGCCTCCTCATAGAGCAAGCTCTGTCTCAGGAGGAAGTCTGTGATTTGCTCCATGCTGCACCCTT CCAAAACATTCTGCCCCGAGTCTACATCAAAGAGGGGGAGCGCCTGGAAGTCCGGATGAAGCGCCTCGAG GCCAAGTACGCCCCACTTCACCTGGTTCCCCTGATAGAGCGGCTGGGGACGCCTCAGCAAATCGCCATCG CTCGGGAGGGTGACCTGCTGACCAAGGAGCGGCTCTGCTGCGGCCTGTCCATGTTTGAGGTCATCCTGAC CCGAATCCGGAGCTACCTGCAGGACCCCATCTGGAGGGGCCCGCCACCCACCAATGGCGTCATGCATGTG GATGAGTGTGTGGAGTTTCACCGGCTGTGGAGTGCCATGCAGTTTGTCTACTGTATCCCTGTGGGGACCA ACGAGTTCACAGCTGAGCAGTGCTTCGGTGACGGCTTGAACTGGGCCGGCTGCTCCATCATTGTCTTGCT GGGCCAGCAGCGGCGCTTTGACCTGTTTGACTTCTGTTATCATCTGCTGAAGGTGCAGAGGCAGGACGGG AAGGATGAGATCATTAAGAATGTGCCTCTGAAGAAGATGGCTGACCGCATCAGGAAGTACCAGATCTTGA ACAATGAGGTTTTTGCCATCCTGAACAAGTACATGAAGTCTGTAGAGACAGACAGTTCCACCGTAGAGCA TGTGCGCTGCTTCCAGCCCCCAATCCACCAGTCATTGGCCACCACCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252460
Insert Size	3762 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252460.1, NP_001239389.1
RefSeq Size	6677 bp
RefSeq ORF	3762 bp
Locus ID	76884
UniProt ID	Q5SQX6
Gene Summary	Part of the WAVE1 complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). Involved in T-cell adhesion and p53-dependent induction of apoptosis (By similarity). Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an additional segment in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein.

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