Ace (NM_001281819) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW208573; CD143
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229518 representing NM_001281819 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGCGTCCGGCCAGCGGGGGCGGTGGCCGTTGTCGCCTCCGCTCTTGATGCTGTCGCTGCTGG TGCTGTTGCTGCAGCCGTCGCCCGCCCCGGCACTCGACCCTGGATTGCAGCCGGGCAACTTTTCCCCGGA CGAGGCAGGGGCGCAGCTTTTCGCTGAAAGCTATAACTCGAGTGCCGAGGTGGTGATGTTCCAGAGCACC GTGGCCAGTTGGGCGCACGACACCAACATCACGGAGGAGAACGCGCGACGCCAGGAGGAAGCGGCCCTGG TCAGCCAGGAGTTTGCAGAGGTCTGGGGCAAGAAGGCCAAGGAGTTGTATGAGTCCATTTGGCAGAACTT TACTGACTCAAAGCTGCGAAGGATCATCGGATCTATTCGGACCCTAGGACCTGCCAATCTGCCCCTGGCC CAGCGGCAGCAGTACAACTCTCTGCTAAGCAACATGAGCAGAATCTACTCCACTGGCAAGGTCTGCTTCC CCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGAGCTCACCAACATCCTGGCTTCCTCACGAAGCTA TGCCAAGTTGTTGTTTGCCTGGGAGGGCTGGCATGATGCTGTGGGTATCCCACTGAAACCCCTCTATCAA GACTTTACTGCCATCAGTAACGAAGCCTACAGACAAGACGACTTCTCAGACACAGGAGCCTTCTGGCGCT CCTGGTATGAGTCCCCCTCCTTTGAGGAGAGTCTGGAACATATCTACCACCAACTAGAGCCCCTCTACCT GAACCTCCATGCCTACGTCCGCCGCGCACTGCACCGCCGCTATGGGGACAAATACGTCAATCTCAGGGGG CCTATTCCTGCCCATTTGCTGGGAGACATGTGGGCCCAGAGCTGGGAGAACATCTACGACATGGTAGTGC CTTTCCCAGACAAACCCAACCTCGATGTCACCAGTACAATGGTACAGAAGGGCTGGAACGCCACACACAT GTTCCGGGTATCAGAGGAATTCTTCACCTCGCTGGGGCTCTCACCCATGCCTCCTGAGTTCTGGGCGGAG TCAATGCTGGAGAAACCAACGGATGGACGGGAGGTGGTGTGCCACGCCTCTGCCTGGGACTTCTACAACC GGAAGGACTTCCGGATTAAGCAATGCACACGGGTCACGATGGAACAGCTGGCCACAGTACACCACGAGAT GGGCCACGTGCAGTACTACCTCCAGTACAAGGACCTGCACGTCTCTCTGCGTAGAGGTGCCAACCCTGGC TTCCATGAGGCCATTGGGGATGTGCTTGCACTCTCCGTCTCTACCCCTGCACATCTGCACAAAATCGGCC TACTGGACCATGTTACCAATGACATAGAGAGTGACATCAATTACCTGCTAAAGATGGCCCTAGAGAAAAT CGCCTTCTTGCCCTTTGGCTACCTGGTGGACCAGTGGCGTTGGGGGGTCTTCAGTGGACGGACCCCACCC TCTCGCTACAACTTCGACTGGTGGTATCTTCGAACCAAGTATCAGGGGATCTGCCCACCAGTTGCCCGGA ATGAAACCCATTTTGATGCTGGAGCCAAGTTTCACATCCCAAACGTGACACCGTACATCAGGTACTTCGT GAGCTTTGTGCTGCAGTTCCAGTTCCATCAAGCACTGTGCAAGGAGGCAGGCCACCAGGGCCCACTACAC CAGTGTGACATCTACCAGTCCGCCCAGGCGGGGGCCAAGCTCAAGCAGGTGTTGCAGGCTGGCTGCTCCA GGCCCTGGCAGGAGGTACTGAAGGACCTGGTAGGCTCAGATGCCCTGGATGCCAAGGCACTGCTGGAGTA CTTCCAACCGGTCAGCCAGTGGCTGGAAGAGCAGAATCAGCGGAATGGCGAAGTCCTAGGCTGGCCAGAG AATCAGTGGCGTCCACCGTTACCCGACAACTATCCAGAGGGCATTGACCTAGAGACTGATGAAGCCAAGG CTGACAGGTTCGTGGAAGAGTATGACCGGACAGCCCAAGTGTTGTTGAACGAGTACGCAGAGGCCAACTG GCAATATAACACCAACATTACCATAGAGGGCAGCAAGATCCTGCTTGAGAAAAGCACGGAGGTATCCAAT CACACCCTGAAATATGGCACCCGGGCCAAGACATTTGATGTGAGCAACTTTCAAAACTCTTCCATCAAGC GGATCATAAAGAAGCTTCAGAACCTGGACCGGGCAGTGCTGCCTCCCAAGGAATTAGAAGAGTACAACCA GATCCTGCTAGACATGGAGACAACTTACAGCTTATCCAACATTTGCTACACAAATGGCACTTGTATGCCC CTGGAACCTGATCTAACAAACATGATGGCCACATCCCGGAAATATGAAGAATTGCTATGGGCATGGAAGA GCTGGAGAGACAAGGTGGGGAGAGCCATCCTTCCTTTTTTCCCAAAGTATGTGGAGTTCTCCAACAAGAT TGCCAAGCTCAATGGCTACACGGATGCAGGGGATTCATGGAGATCCTTATACGAGTCTGACAACCTGGAG CAAGACCTGGAAAAACTGTACCAGGAGCTGCAGCCACTCTACCTGAACCTGCATGCCTATGTGCGTCGTT CCCTGCACCGCCACTATGGGTCCGAGTACATCAACCTGGATGGCCCCATTCCTGCCCATCTGCTAGGGAA CATGTGGGCGCAGACCTGGTCCAACATCTATGATTTGGTGGCGCCCTTCCCTTCCGCCCCCAATATAGAT GCCACGGAAGCCATGATAAAGCAGGGATGGACACCCAGAAGGATATTTAAGGAAGCTGACAATTTCTTTA CCTCCCTGGGGCTGTTACCTGTGCCCCCTGAGTTCTGGAACAAGTCGATGTTAGAGAAGCCCACCGATGG AAGGGAGGTGGTGTGCCATCCCTCAGCCTGGGACTTCTACAACGGCAAGGACTTCAGGATCAAGCAGTGT ACCTCTGTGAACATGGAGGACTTGGTGATAGCGCACCACGAAATGGGCCACATCCAGTATTTCATGCAGT ACAAAGACTTACCCGTGACTTTCCGGGAGGGTGCCAACCCTGGTTTTCATGAAGCTATTGGAGATATAAT GGCTCTCTCAGTGTCTACCCCCAAGCATCTATACAGTCTCAACCTGCTTAGCACTGAGGGCAGTGGCTAC GAGTATGACATCAACTTTCTAATGAAGATGGCCCTCGACAAGATCGCCTTTATCCCCTTCAGCTACCTCA TCGACCAGTGGCGCTGGAGGGTCTTTGATGGAAGCATCACCAAGGAGAACTATAACCAGGAGTGGTGGAG CCTCAGGCTGAAGTATCAGGGTCTGTGCCCCCCAGTGCCAAGATCCCAAGGTGACTTTGACCCAGGGTCC AAGTTCCACGTTCCTGCGAACGTGCCATACGTCAGGTACTTTGTCAGCTTCATCATCCAGTTCCAGTTCC ACGAGGCGCTGTGTCGCGCAGCCGGGCACACGGGTCCCCTGCACAAGTGTGACATCTACCAATCCAAGGA AGCAGGGAAGCTCCTGGCGGATGCCATGAAGCTGGGCTACAGTAAGCCGTGGCCAGAGGCCATGAAGCTG ATCACAGGCCAGCCTAACATGTCAGCCTCCGCCATGATGAATTACTTCAAGCCACTGACAGAATGGCTCG TCACCGAGAACAGGAGACATGGAGAGACACTGGGCTGGCCGGAGTACAACTGGGCGCCAAACACCGGTAC TACACCCACCCTGCCTCCAGCCCCCGGGCCCAGCTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281819
Insert Size	3750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281819.1, NP_001268748.1
RefSeq Size	5091 bp
RefSeq ORF	3750 bp
Locus ID	11421
Gene Summary	Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate segment in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.

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