Dctn1 (NM_001198867) Mouse Untagged Clone
CAT#: MC229506
Dctn1 (untagged) - Mouse dynactin 1 (Dctn1), transcript variant 3
CNY 11,970.00
货期*
6周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AL022633; DAP-150; DP-150; Glued; p150; p150-glued; p150 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC229506 representing NM_001198867
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACGGAGGCAAGCGCCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACC GAGGCACTGTGGCCTATGTTGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGA AGCAAAAGGCAAAAATGATGGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATC TTTGTACGCCAGTCCCAGATCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACTCCTGATT CTTCTGCTTCAAAGGTCCTCAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCCTACTCG CCCAGCCAGCACTGGGGTGGCTGGGCCCAGTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAA CTAAGCAGCAGTGAGCCCAGCACCCCAGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCC TCACCTCTCCTGGAGCAGCACCCCCACTTCCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGT ACGGGACCTGGAGGAGAAGCTGGAGACCCTGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAG CTGGAGAAGCACAAGATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAG ACCTGCAGCGGCGCCTCAAGGAGGCTCGGAAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACAT GGAGGAGATGGCCGACACAGCCGACGCTATCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGC GCTGAGTCTCTGCAGCAAGAGGTGGAGGCACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGA TTCTCAAGGCTGAAATCGAAGAGAAAGGCTCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGA GGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAG CACGTGAAGCTGCAGAAACTCATGGAAAAGAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGC GTCTTCAGGAGGAGCTGAGCCAGGCTGAGAGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCT GGGAGCCGAGGAGATGGTGGAGATGCTGACCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTA CGGGAGACTGTGGGGGACTTGGAAGCCATGAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGA CGGAGCTGGAACTCCGAGAGCAGCTGGACATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGA AGCCGCCCAGGAGACAGTCGCCGACTACCAGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTA CAGGATGTCAATCGGGAGCTGACAAACCAGCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAG AGACTTTTGATTTCAAAATCAAGTTTGCTGAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAG ACAGATGGAAGTTGCCCAGGCCAACCGGCACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTT CGGCCAGGTGGAGACCACGACTGTGTCCTGGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGC TCATCCGGAAGCAGGCCCAGGAGAAGTTTGACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGG AGCTGCCGGGGAGCAGCTGAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTG CACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCG AGATGAGCGCCCACGAGCGCTCCTTAGATTTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGAC TGTCAACGTGGAGCCCCTCACCAAGGCCATCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAA CAACCCGAGGACTCCACCATGCAGCTGGCTGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGG GCGTGGAGGTGGGGCGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCT CCGAGACCTGGAAACATCATGTAGTGACACCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGG ACGGATGCTCCTGGGATCCCAGCAGCGCTGGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCA GGAAGCACTTGACGTGGGTGGTAGCTGTTCTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCC CTTGGCAGAGAACGAGGGGCTGCCTGTGGCTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATC TACGGGAGCCCCTCCAGCAGCCCCTATGAGTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGA ACAAGCTGGCCACAGCCATGCAAGAAGGCGAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGT TGAACTTCGGGCTGCAGCCCTGCGTGCAGAGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGAT CGAGAGACAGTTATCAAGGAGTTAAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACG TGCGGCTCAGCCTCCTGGAGAAGAAGTTGGACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGT TCAGACACGGCTGGACGAGACTCAGACCCTGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGAC GCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGT CCAAGCGCACAATCGAGGGGCTCCGGGGCCCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGC TGGTGGGGGCGCTCCTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCCCACTG CTGCTTCAGCAGATCTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCTCAGAG GAGCCCAGATGAAGGCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCACCCCA TGAGGGCCCCGGTGGTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGAAACTA AACCAGCTGAGTACCCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCCGTCAG CTCAGCTTATGGAACAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGATGAGGT CCTCAAGGAGACAGTGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTTCATCT GCCTTCCTCAGGGCCAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTCATGCG CGGCAGGCCTAGGACAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCT CATCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001198867 |
Insert Size | 3720 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001198867.1, NP_001185796.1 |
RefSeq Size | 4172 bp |
RefSeq ORF | 3720 bp |
Locus ID | 13191 |
Gene Summary | Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR,lacks multiple exons in the coding region, and uses an in-frame downstream start codon, compared to variant 1. Variant 3 encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR231717 | Dctn1 (myc-DDK-tagged) - Mouse dynactin 1 (Dctn1), transcript variant 3 |
CNY 12,350.00 |
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