Apaf1 (NM_001282947) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229505 representing NM_001282947 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG TGTCGTCTTCCAGTGTAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGGTTATTTTCGTTAC TAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTCTGGAAACTGAATGGAGAACCAGGGTGGGTCACC ATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAGCTGCGGAAGCCGTTCGAGATCACTCCCTCTTAG AAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCATTGGAAAACAAGACAAATCTGGGCTTCTCATGAA ACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAGAGTTTCTCTCAGAGGCTTCCACTTAATATTGAG GAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCAAACACCCAAGGTCTCTGTTGATCTTGGATGATG TTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCAGTGTCAGATTCTTCTTACAACCAGAGATAAGAG TGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTCCCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGA CTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAGAAGATCTGCCAGCGGAGGCTCACAGTATTATAA AGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAATTGGTGCGCTTTTACGTGATTTTCCCAATCGCTG GGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAGTCTTCATCTTATGATTAT GAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGACTATTACACAG ACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTACAAAGGTGTTGTGCGTTCTCTGGGACTTGGAAAC GGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAATAAGTCTCTCTTATTCTGTAATCGGAATGGAAAG TCATTTTGTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATCGCAGTCAGCTTCAGG ATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTATTACCAGCCCCACACGCTGTCTCCAGACCAGGA GGACTGCATGTATTGGTACAACTTCCTAGCCTATCACATGGCTAGTGCCAATATGCACAAAGAACTTTGT GCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAACAGAACTTGTCGGCCCTGCCCATCTGATTCACG AGTTCGTGGCATATAGGCATATATTGGATGAAAAGGATTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTT ATCTTTAAATGGACACCTCCTTGGACGACAGCCATTTCCTAATATTGTACAGCTGGGCCTCTGTGAACCA GAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGGCCAAGCAGGAGGGGGATACTGGGCGCCTTTACC TGGAATGGATAAACAAAAAAACTATCAAGAATCTGTCCCGCTTAGTCGTCCGCCCCCACACAGATGCTGT TTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATAGCTTCTTGTGGGGCTGATAAAACCTTACAGGTG TTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCAAAGCTCACGAAGATGAGGTGCTTTGCTGCGCGT TCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGCGGATAAGAAGGTTAAGATTTGGGATTCTGCGAC TGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAGCAAGTCAATTGCTGCCATTTCACCAACAAGAGT AACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCTTCCTCAAGCTCTGGGATTTGAATCAAAAAGAAT GTCGAAATACCATGTTTGGTCACACGAACTCAGTCAACCACTGCAGGTTCTCACCAGACGATGAGCTCTT GGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGGGATGTGAGATCAGCAAACGAGAGGAAAAGCATT AATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTCCAGAGGATGTGGAGGTGATCGTGAAGTGTTGTT CCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGCAAAAAACAAAGTCCTCCTTTTTGATATTCATAC TAGTGGCCTATTGGCAGAGATCCACACAGGCCATCACAGCACCATCCAGTACTGTGACTTCTCCCCCTAT GACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTGTGGAGTTGTGGAACATAGACTCCCGCCTAAAGG TGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGGTGTGATGTTTTCTCCCGATGGCTCCTCATTTTT GACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAGACAAAAAAGGTATGCAAGAACTCTGCCATCGTG CTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACGAAACGATGGTCCTTGCAGTTGACAACATAAGAG GCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGATTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTG CCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGATGAAGATGGAGCCATTAAGATTATAGAACTTCCA AACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGAAAGCTGTGCGGCACATCCAGTTCACAGCTGATG GGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGATTCAGGTATGGAATTGGCAGACAGGGGACTATGT ATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTCAGGCTCCTCCAAGATTCAAGATTGCTTTCTTGG TCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTACCGGAAGAATAGAAAGAGACTTTACTTGTCATC AGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGCGACCAAGTTTTCATCTACCTCTGCTGATAAGAC TGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTTCATGAGCTGAAGGGCCATAATGGCTGTGTCCGC TGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAACTGGAGATGACAATGGAGAAATCCGGATATGGA ATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCCGATCTCGGTAGAGGAAGGAACTGCTACCCACGG CGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGTAAAACGCTTGTCTCTGCTGGAGGATATCTCAAG TGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCTTCTACACAAATGGAACAAACCTCAAGAAAATCC ACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGATAATCTCGGTATTTTATATATTTTACAGGTTTT AGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282947
Insert Size	3717 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282947.1, NP_001269876.1
RefSeq Size	6497 bp
RefSeq ORF	3717 bp
Locus ID	11783
Gene Summary	Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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