Apaf1 (NM_001282947) Mouse Untagged Clone
CAT#: MC229505
Apaf1 (untagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3
CNY 11,970.00
货期*
6周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC229505 representing NM_001282947
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG TGTCGTCTTCCAGTGTAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGGTTATTTTCGTTAC TAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTCTGGAAACTGAATGGAGAACCAGGGTGGGTCACC ATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAGCTGCGGAAGCCGTTCGAGATCACTCCCTCTTAG AAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCATTGGAAAACAAGACAAATCTGGGCTTCTCATGAA ACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAGAGTTTCTCTCAGAGGCTTCCACTTAATATTGAG GAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCAAACACCCAAGGTCTCTGTTGATCTTGGATGATG TTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCAGTGTCAGATTCTTCTTACAACCAGAGATAAGAG TGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTCCCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGA CTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAGAAGATCTGCCAGCGGAGGCTCACAGTATTATAA AGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAATTGGTGCGCTTTTACGTGATTTTCCCAATCGCTG GGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAGTCTTCATCTTATGATTAT GAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGACTATTACACAG ACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTACAAAGGTGTTGTGCGTTCTCTGGGACTTGGAAAC GGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAATAAGTCTCTCTTATTCTGTAATCGGAATGGAAAG TCATTTTGTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATCGCAGTCAGCTTCAGG ATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTATTACCAGCCCCACACGCTGTCTCCAGACCAGGA GGACTGCATGTATTGGTACAACTTCCTAGCCTATCACATGGCTAGTGCCAATATGCACAAAGAACTTTGT GCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAACAGAACTTGTCGGCCCTGCCCATCTGATTCACG AGTTCGTGGCATATAGGCATATATTGGATGAAAAGGATTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTT ATCTTTAAATGGACACCTCCTTGGACGACAGCCATTTCCTAATATTGTACAGCTGGGCCTCTGTGAACCA GAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGGCCAAGCAGGAGGGGGATACTGGGCGCCTTTACC TGGAATGGATAAACAAAAAAACTATCAAGAATCTGTCCCGCTTAGTCGTCCGCCCCCACACAGATGCTGT TTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATAGCTTCTTGTGGGGCTGATAAAACCTTACAGGTG TTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCAAAGCTCACGAAGATGAGGTGCTTTGCTGCGCGT TCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGCGGATAAGAAGGTTAAGATTTGGGATTCTGCGAC TGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAGCAAGTCAATTGCTGCCATTTCACCAACAAGAGT AACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCTTCCTCAAGCTCTGGGATTTGAATCAAAAAGAAT GTCGAAATACCATGTTTGGTCACACGAACTCAGTCAACCACTGCAGGTTCTCACCAGACGATGAGCTCTT GGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGGGATGTGAGATCAGCAAACGAGAGGAAAAGCATT AATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTCCAGAGGATGTGGAGGTGATCGTGAAGTGTTGTT CCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGCAAAAAACAAAGTCCTCCTTTTTGATATTCATAC TAGTGGCCTATTGGCAGAGATCCACACAGGCCATCACAGCACCATCCAGTACTGTGACTTCTCCCCCTAT GACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTGTGGAGTTGTGGAACATAGACTCCCGCCTAAAGG TGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGGTGTGATGTTTTCTCCCGATGGCTCCTCATTTTT GACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAGACAAAAAAGGTATGCAAGAACTCTGCCATCGTG CTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACGAAACGATGGTCCTTGCAGTTGACAACATAAGAG GCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGATTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTG CCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGATGAAGATGGAGCCATTAAGATTATAGAACTTCCA AACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGAAAGCTGTGCGGCACATCCAGTTCACAGCTGATG GGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGATTCAGGTATGGAATTGGCAGACAGGGGACTATGT ATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTCAGGCTCCTCCAAGATTCAAGATTGCTTTCTTGG TCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTACCGGAAGAATAGAAAGAGACTTTACTTGTCATC AGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGCGACCAAGTTTTCATCTACCTCTGCTGATAAGAC TGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTTCATGAGCTGAAGGGCCATAATGGCTGTGTCCGC TGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAACTGGAGATGACAATGGAGAAATCCGGATATGGA ATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCCGATCTCGGTAGAGGAAGGAACTGCTACCCACGG CGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGTAAAACGCTTGTCTCTGCTGGAGGATATCTCAAG TGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCTTCTACACAAATGGAACAAACCTCAAGAAAATCC ACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGATAATCTCGGTATTTTATATATTTTACAGGTTTT AGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-RsrII |
ACCN | NM_001282947 |
Insert Size | 3717 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282947.1, NP_001269876.1 |
RefSeq Size | 6497 bp |
RefSeq ORF | 3717 bp |
Locus ID | 11783 |
Gene Summary | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. |
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR231716 | Apaf1 (myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3 |
CNY 12,350.00 |
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