Nek1 (NM_001293638) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D8Ertd790e; kat
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229492 representing NM_001293638 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATGTGAGACTGCAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGA CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGA ATCAAGGAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATCATTT GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTTTAAACGAATAAATG CTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTTTGTGCAGATATGTTTGGCTCTGAA GCATGTACATGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGCACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTG GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC CTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTATGATCTCCGCAGCT TGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT TATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACACTT TCAAAGTTTGGACCACAGCCTCTCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC CTGCTCAGAAAATCACAAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAA AAAGTTACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAATGAAT TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGAATTTATTGAGAAAG AAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGATGAAGCGGCAAGAGAAGCAGCGGTT GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA AAGGCTTCCTTTTTTGGCATTGGAGGGGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAAC ATTACCATGCCATTTTTGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGG AATCTATGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGTGGAA GAATTCCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACACGTGGGACTCCTGC AAAACCTGGCATCTCTGTATGGAGGCAGGCCCAGCTCTTCAAGAGGAGGGAAGCCAAGGAACAATGAGGA AGAGGTTTATTTGGCAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCC AAACTTCGTGGTGAGAATAAAGAAGCTGATGGTACCAAAGGACAAGAAGCAACTGAAGAGACTGACATGA GGCTCAAAAAGATGGAGTCACTTAAGGCGCAAACAAATGCACGTGCTGCTGTACTAAAAGAACAGCTGGA GCGAAAAAGAAAGGAAGCTTATGAAAGAGAAAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAA AGCTCAGATGTTCCTCTGCCTTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGC CTGTCATTTCTGTGACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGA AGAAATGGAAAAGAGTAACAGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAATGAAAATCTT AAAGCTCAAGAGGATGAAAAGGAAAAGCAGCATCACTCAGGTTCTTGTGAGACCGTTGGTCACAAAGATG AGAGAGAGTATGAGACAGAAAATGCCATTTCCTCTGATCGCAAGAAGTGGGAGATGGGAGGTCAGCTTGT GATTCCTCTCGATGCAGTGACACTGGATACATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTT ATTAAATTAGATTCTAATGGCTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGA TACTTGGAGAAGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTA TGCTGAAGAGGAGAACTACAAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTCAAAAGAAATA AATCCATCAGCTACTGTTGATTCTACTGAAACGAAAAGTCCAAAGTTTACTGAGGTGTCTCCACAAATGT CAGAAGGAAATGTGGAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTAGCACACACAC AGATGGGAGTTTGCCACCTGTTCTTAATGATGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAG TTGGAAGATAAGGTTGCTGTGCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAAT CCTGTAAGGATCAGAGAGATCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGT ACAGCCAGAATCGATTTTCCAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGCAGTTCATTGC TCACCAGAAGAACCAATTCCAATTCGATCTCACTCTGATTCTCCACCAAAAACTAAGAGCAAGAATTCCT TACTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAATCCAAAGATGCTGAGGACCTGCTCACTTCC AGATCTTTCCAAGCTGTTCAGAACCCTAATGGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTT GAAATCGATGAGCTGGAAGATGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAG AAACTGACACAGATTTACAAGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGA ATACAGTGAGGAGGAAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGGGACAGATGCC CCAGACGAGGAGGACAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGATAATAGTGACG CTGAGACCACTAGTGAATGTGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGA GCAAGAAATGGGCTTTGAAAAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGAT GAAAATATTGAAATTTGTTCAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCA AGATTCTGCATTTAGTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293638
Insert Size	3696 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293638.1, NP_001280567.1
RefSeq Size	5503 bp
RefSeq ORF	3696 bp
Locus ID	18004
UniProt ID	P51954
Gene Summary	Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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