Ehmt2 (NM_001286573) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bat8; D17Ertd710e; G9a; KMT1C; NG36
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229489 representing NM_001286573 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGTCTGCCGAGAGGGAGGGGGCTGATGCGGGCCCGGGGGCGGGGGCGTGCGGCCCCCACGGGCG GCCGCGGCCGCGGTCGGGGGGGCGCCCACCGAGGGCGAGGTAGGCCCCGAAGCCTGCTCTCGCTGCCCAG GGCCCAGGCGTCTTGGGCCCCCCAGCTGCCTGCCGGGCTGACCGGCCCCCCGGTTCCTTGTCTCCCCTCC CAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAG TTCATAGCTCTTTGGGGGACACCCCTCAGAGTGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGA GCCTGCCGGCCCCTCCTCTCCGGCCTCTGTCACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTC GGGGCCGCATCACTCATCGGGGACGAACCCGAGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCC ATGCCACAAAGTCGTTCCCCTCTTCCCCCAGCAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAAT GACAGGGGCAGGAAAGTCGCCCCCCTCGGTCCAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCC CAGGGAGCTGCAACTGCTGGGCCTGAACCCTCTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAG TGCACCGAGCCCGGAAAACCATGTCCAAACCTAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCC TGAAGTCCAGCATTTCCGCATGAGTGATGACATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGG AGGAAGCTGAACTCTGGTAGCCTGTCCGAGGACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGG AGAAGGGAGAGCCCAGGCCCCTGGAGGAGTGGGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGA TGCGTACTCTGTGGATGAGCGGGTGGACTCTGACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTG AGTGAGGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGG AAGAAGAGGACGAGGAGTCGGGCAATCAGTCAGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAA GAAATGGCGGAAAGACAGCCCGTGGGTGAAGCCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCC AAGGAGCCAAGAGGGGTCTCCAATGACACGTCTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCCC TCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGGC CACAGAGAGTGTGGATGGAGAGCTCCTGGGCTGCAATGCTGCCATCCTTAAGCGGGAGACCATGCGGCCG TCTAGCCGCGTGGCGCTGATGGTGCTCTGTGAGGCCCATCGAGCCCGCATGGTCAAGCACCATTGCTGCC CGGGCTGCGGCTACTTCTGCACAGCGGGCACCTTCCTGGAATGCCACCCCGACTTTCGTGTAGCTCACCG CTTCCATAAGGCCTGCGTATCCCAGCTCAATGGGATGGTCTTCTGTCCCCACTGTGGAGAGGATGCCTCA GAGGCCCAGGAGGTGACCATTCCTCGGGGCGATGGGGGAACACCCCCAATTGGCACCGCAGCTCCTGCTC TGCCACCCCTGGCACATGATGCCCCAGGGCGAGCGGATACCTCCCAGCCTAGCGCCCGAATGCGAGGGCA TGGAGAGCCGCGGCGCCCGCCCTGTGATCCCCTGGCTGACACCATCGACAGCTCAGGGCCTTCACTGACT CTGCCTAATGGGGGCTGCCTCTCCGCTGTGGGTCTGCCCCCAGGGCCGGGCAGGGAAGCCCTGGAAAAAG CCTTGGTCATCCAGGAGTCTGAGAGGCGGAAGAAGCTGCGATTCCACCCACGGCAGCTGTACCTGTCGGT GAAGCAGGGGGAGCTGCAGAAGGTGATCCTTATGCTGTTAGACAACCTGGACCCCAACTTCCAGAGCGAC CAGCAGAGCAAGCGCACGCCCCTGCACGCGGCCGCCCAGAAGGGGTCGGTAGAGATCTGTCATGTGCTGC TGCAGGCAGGAGCCAACATCAATGCCGTAGATAAGCAACAACGCACGCCACTAATGGAGGCCGTGGTGAA CAACCACCTGGAGGTGGCACGCTACATGGTGCAGTTAGGTGGCTGTGTCTACAGCAAGGAAGAGGATGGC TCCACCTGTCTACATCATGCAGCCAAAATTGGGAACTTGGAAATGGTCAGCCTGCTACTGAGCACAGGAC AGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACGCCCATCATCTGGGCAGCCGAGCACAAGCACAT CGATGTGATTCGTATGCTGCTGACCCGGGGTGCCGATGTCACCCTGACTGACAATGAGGAAAACATCTGC CTGCACTGGGCCTCCTTCACGGGTAGTGCCGCCATCGCTGAGGTCCTTCTGAATGCCCAGTGTGATCTCC ATGCTGTCAACTACCATGGGGACACGCCCCTGCACATAGCCGCCAGGGAGAGCTACCATGACTGTGTTCT GTTGTTCCTGTCTCGTGGAGCCAACCCTGAGCTTCGGAACAAAGAAGGAGACACGGCATGGGATCTGACC CCAGAGCGCTCTGATGTGTGGTTTGCACTGCAGCTCAATCGAAAGCTTAGGCTTGGGGTAGGGAACCGGG CTGTCCGCACCGAGAAGATCATCTGCCGGGACGTAGCCCGAGGCTATGAGAATGTACCCATCCCCTGTGT CAATGGTGTGGATGGGGAGCCGTGCCCGGAGGACTACAAGTACATCTCTGAGAACTGCGAGACATCGACC ATGAACATCGACCGCAACATCACCCATCTGCAGCACTGCACGTGTGTGGATGACTGCTCCAGCTCCAATT GCCTATGTGGTCAGCTCAGTATCCGATGCTGGTATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACAA GATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCATGCTCCTGCTGGAGAAGCTGCAAGAACCGCGTG GTGCAGAGCGGCATCAAGGTACGGCTGCAGCTCTACCGGACTGCCAAGATGGGCTGGGGGGTCCGAGCCT TGCAGACCATCCCCCAGGGCACGTTCATCTGCGAGTATGTAGGAGAGCTGATCTCTGATGCCGAGGCTGA TGTGAGAGAGGATGATTCTTACCTCTTCGATTTAGATAACAAGGATGGCGAGGTTTACTGCATTGATGCC CGTTACTATGGCAACATCAGCCGATTCATTAACCACCTGTGTGACCCCAACATCATCCCTGTCCGGGTTT TCATGCTGCACCAAGATCTACGGTTCCCACGCATTGCCTTCTTCAGCTCCAGGGACATCCGGACTGGGGA GGAGCTGGGCTTTGACTACGGTGACCGATTCTGGGACATCAAGAGCAAGTATTTCACCTGCCAGTGTGGC TCTGAGAAGTGCAAGCATTCAGCGGAGGCCATCGCCCTGGAGCAGAGCCGCCTGGCCCGGCTGGACCCCC ACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCCATCAACACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286573
Insert Size	3690 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286573.1, NP_001273502.1
RefSeq Size	3968 bp
RefSeq ORF	3690 bp
Locus ID	110147
UniProt ID	Q9Z148
Gene Summary	Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame internal coding exon, compared to variant 1. The resulting isoform (c) lacks an internal segment, compared to isoform a.

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