Kcnt1 (NM_001302351) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C030030G16Rik; s; Sl; Slack; slo2; Slo2.2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229486 representing NM_001302351 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGCCAAGCTGCCGCGCTCGCCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCG CTGCAGCCCCTGAGGAGCCACACGGGCTCAGCCCGCTACTGCCGGCCCGCGGCGGGGGCTCCGTGGGCAG CGACGTGGGCCAGAGGGTCCAGGTGGAGTTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTG TTCTTCATCAAAAACCAAAGATCCAGCCTGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCT GCCTGCTGTATATCGTCCGTGTCCTGCTTGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAA GTATAACTACACTTTCAATGGCTCATCCTCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAA ATGGCTCTGTGGGTGATCCAGGTCATTGTGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACC TCAGCTACAAAGGCAACATCTGGGAGCAGATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACT GCCCTTCATCATCACGGTCTTCTGGCCACCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGG CTGGCCAAGCATGCGCTGGAGAACATGATTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCA TGTTCAACCAGGTGCTCATCCTGTTCTGCACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCA GCACCTGGAGCGAGCAGGTGGCAACTTGAACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCA ACTGTGGGCTTCGGTGATGTGACGCCCAAGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTG TCACCCTTGTGGTGCTCCCACTGCAATTTGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGG GGGCAACTATAGCCGCCACCGAGCACGGACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATT GATCTCCTCATGGACTTCCTGAATGAGTTCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCC TGTGTCCCTCTGAAATGGACGTCCAGGTGCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCAT CTACCTCCAGGGCTCTGCCCTCAAGGACCAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGC TTTATCCTCAGCAGCAGGAATGAGGTGGACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGG CTGTGAAGGACTTTGCCCCCAACTGTCCCCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGT CAAATTTGCTGACCACGTGGTATGCGAGGAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGC CCGGCCACCTCCACCCTCATCACCCTGCTGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCG AGCAGTGGCAGCGCACGTACGGGAGGTGCTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAA ATTCTTCCGGGAGTATGAGGGCAAGAGCTTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTG TGCCTCATCGGGCTGAAGCGTGAGGAGAACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGG CTGCCTCCGACACCTGCTTCTATATCAATATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGA GGAGAAGCAGAAGAGACGGGGCCTTGCAGGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCAT AGCATCATCGCCTCTATGGGGACAGTGGCCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTG GCAGTGGCGGGGACGGCACAAAGCTGACTCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCAT CGCACCCGTTCTGGAGTTGGCAGACAGCTCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAG GATGAGGTGACACCCTCGGACGACGAGGGGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACT CACCCTACATTGGCAGCTCCCCGACTTTATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCG GTTGGACAAGGGCTGCAAACACAACAGCTACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATT ATTGTCTCTGCTGAGACGGCAGGCAACGGGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGT CCCGCCGGGAGCTCAACCCTATCGTGTTGCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCAT CTGTTGCTTCCCCATGGTCTACTACATGGAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGC ATCATCTATGCTGACAACCTGGTGGTGGTGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGG CAGATGCCAAGACCATCGTCAATGTGCAAACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGA GCTCACACACCCTTCCAACATGCGGTTCATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCC AAACTTGAAAAGCAAGAACGGGAGAACGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTG GTCGAGTATTTAGTATCAGCATGTTGGATACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCAC CATCACCAGGCTGCTCTTGGGCCTTGATACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACC GAGGACGACCTGTGGATCCGCACTTACGGCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCC CCATCGGCATCTACAGGACCGAGTGCCATGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGAT CTCGGTGAACATGGAGGACTGCGAGGATACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCT GGCAGTGGCAGCACCCATGGCCGTCACGGGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCA AGAGCCTGCAGTGGGCCCGCAAGCTGAGTCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCAC AGACTGGATCACCCAGCAGCGGCTCAGCCTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTC AAGAACCGAATGAAGCACCTGGGACTGCCCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTG ATGTGATGAATCGGGTAAACCTGGGATATTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTC CTATGTACTCATCAACCCCCCGCCAGACACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCC GACCCCCTGGCCCATGTGGCCAGCAGCTCCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCT GTAATCCTGAGACCAGGGATGAGACCCAGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302351
Insert Size	3675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302351.1, NP_001289280.1
RefSeq Size	5945 bp
RefSeq ORF	3675 bp
Locus ID	227632
UniProt ID	Q6ZPR4
Gene Summary	This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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