Brpf1 (NM_001282128) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4833438B11Rik; 4930540D11Rik; Brpf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229482 representing NM_001282128 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTGGACTTTGACGTGAAGACCTTCTGCCACAATTTGCGGGCAACTAAGCCACCATATGAGTGCC CTGTGGAGACTTGCCGCAAGGTTTACAAGAGTTACAGTGGTATCGAGTACCACCTGTACCACTATGACCA CGACAGCCCACCACCCCCACAGCAGACCCCACTGCGCAAGCACAAAAAGAAAGGGCGCCAGTCACGACCA GCCAACAAGCAGTCACCCAGCCCCTCTGAAGTCTCACAGTCACCAGGCCGAGAGGTGATGAGCTATGCTC AGGCCCAGCGCATGGTAGAAGTGGACCTTCATGGCCGTGTCCACCGAATCAGCATCTTTGACAACTTGGA TGTGGTGTCAGAGGATGAGGAGGCCCCTGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAAAACACTGAG ACACCTGCGGCTACACCTAAGTCAGGCAAGCATAAGAACAAGGAGAAACGAAAAGACTCTAACCACCACC ATCACAGCGCTCCTGCCAGTGCTGCTCCCAAATTGCCTGAGGTGGTGTATCGTGAGCTAGAGCAAGATAC CCCTGACGCACCACCCCGGCCCACTTCCTACTACCGGTACATCGAGAAATCTGCAGAGGAGCTGGATGAG GAGGTGGAGTATGACATGGATGAAGAGGACTATATCTGGCTGGATATCATGAATGAGCGGCGGAAGACTG AGGGTGTAAGTCCCATCCCACAAGAGATCTTTGAGTACTTAATGGACCGTTTGGAGAAGGAGTCGTACTT TGAGAGTCACAATAAAGGTGACCCCAATGCACTAGTGGATGAAGATGCCGTGTGCTGTATCTGCAATGAT GGCGAGTGCCAGAACAGCAATGTTATCCTCTTCTGTGACATGTGTAACTTGGCTGTGCACCAGGAGTGCT ACGGTGTCCCCTATATCCCTGAAGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCTTCTCGTGCAGT GGATTGTGCTCTGTGCCCCAATAAGGGTGGTGCCTTCAAGCAGACAGATGATGGCCGCTGGGCCCACGTG GTGTGTGCCTTGTGGATCCCTGAGGTTTGCTTTGCCAACACAGTCTTCCTAGAACCTATTGACAGCATTG AGCACATCCCACCAGCTCGGTGGAAGCTCACCTGCTACATTTGTAAACAGCGGGGCTCTGGAGCCTGCAT CCAGTGCCATAAGGCCAATTGCTACACAGCCTTCCATGTGACATGTGCCCAACAAGCTGGCCTTTACATG AAGATGGAACCTGTGCGGGAGACAGGTGCCAATGGTACCTCCTTTAGCGTCCGCAAGACAGCCTACTGTG ACATCCACACACCCCCAGGTTCTGCTCGTCGCCTGCCTGCCCTATCCCACAGTGAGGGTGAGGAAGAAGA GGATGAAGAAGAAGATGAGGGTAAAAGCTGGAGCTCAGAGAAGGTCAAGAAGGCCAAGGCCAAGTCTCGG ATTAAGATGAAGAAAGCTCGGAAGATCTTGGCAGAGAAGAGGGCAGCAGCACCTGTGGTGTCCGTGCCCT GCATCCCGCCACACAGGCTCAGTAAGATCACCAACCGCCTGACCATCCAGAGGAAGAGCCAGTTCATGCA GAGGCTGCACAGCTACTGGACTCTGAAACGACAATCACGGAATGGGGTCCCACTACTCAGGCGCCTACAA ACACACCTTCAGTCTCAGAGGAACTGTGAACAAGTTGGGAGAGATTCTGATGATAAAAACTGGGCCCTCA AAGAACAGCTCAAGTCCTGGCAGAGACTGCGGCATGACCTGGAGCGAGCTCGGCTGCTGGTGGAGCTGAT CCGCAAGCGAGAGAAACTAAAAAGGGAGACGATCAAGATCCAGCAGATTGCCATGGAGATGCAGCTGACC CCTTTCCTCATCCTCCTCCGAAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCG AGCCGGTCCCTCTGTCTGAGGTAACCGAATTGGACGAAGTACCCGACTACCTAGACCACATCAAAAAGCC CATGGACTTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACTTGAACTTTGATGATTTTGAGGAG GACTTCAACCTCATTGTCAGCAACTGCCTAAAGTATAATGCCAAGGACACCATCTTCTACAGGGCAGCAG TGCGACTCCGTGAGCAGGGTGGTGCTGTGCTCCGTCAAGCCCGGCGACAGGCAGAAAAAATGGGCATTGA CTTTGAGACGGGCATGCATATCCCTCACAACCTGGCCGGAGATGAGGTCTCACACCACACTGAAGATGTA GAGGAAGAACGGCTGGTCCTGCTGGAGAACCAGAAACACCTGCCAGTAGAAGAACAGCTGAAGTTGTTGC TGGAGAGGCTGGATGAAGTCAATGCCAGCAAGCAGAGTGTGGGCCGCTCTCGGCGTGCAAAAATGATCAA GAAGGAGATGACAGCATTGCGGCGGAAGCTTGCTCACCAGCGGGAGACTGGCCGGGATGGGCCTGAGCGT CATGGCCCCTCAGGTCGGGGCAATCTGACACCCCACCCAGCAGCCTGTGACAAAGATGGACAGACCGACA GTGCTGCAGAAGAGAGCAGCAGCCAGGAAACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGC ACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAG AGGCCGGGCCGGCCCCCAAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGC CCCCTCAGCTCCCCATCATGGGCTCCCTACGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCAAG CTCAGACAGCGACAGTGATAAGTCCACAGAAGATCCCCCAATGGACTTACCAGCCAATGGCTTCAGCAGT GGGAACCAGCCAGTGAAGAAGAGTTTCTTGGTGTACCGTAATGACTGCAACCTTCCCCGAAGCAGCTCAG ACTCTGAGTCCAGCAGCAGCAGCAGCAGCAGTGCAGCCTCAGACCGGACCAGCACAACTCCCTCAAAACA AGGCAGGGGCAAGCCCTCTTTCTCTCGGGGCACATTCCCAGAAGACAGTAGTGAAGATACCTCAGGCACT GAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCATGGTAAGAAAGAGTCTGGGTC GAGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGTTGGATGCTCTGGACCTCGTGTGGGCCAAATG CCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCAT GGGGTTCCTATCCCTGTACCACCACTGGAGGTTCTAAAACTTGGGGAACAAATGACACAGGAAGCCCGAG AGCATCTCTACCTCGTTCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCCGGACTAAGCTTGT TCCTCTGGGTGTGAACCAGGATCTAGACAAAGAGAAGATGCTGGAGGGCCGCAAGTCCAACATCCGCAAG TCAGTGCAGATTGCTTACCACAGGGCTCTGCAGCACCGAAGCAAGGTGCAGGGTGAGCAGAGCAGCGAGA CCAGCGATAGTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282128
Insert Size	3657 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282128.1, NP_001269057.1
RefSeq Size	4796 bp
RefSeq ORF	3657 bp
Locus ID	78783
Gene Summary	Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Preferentially mediates histone H3-K23 acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice site, and lacks an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1.

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