Spag9 (NM_001199205) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110018C07Rik; 4733401I23Rik; 4831406C20Rik; AW552012; Jip4; JLP; JSAP2; JSAP2a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229445 representing NM_001199205 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTGGATGCATGCTGCTGTTTGTGTTTGGCTTTGTTGGCGGGGCGGTGGTCATTAATTCTGCGA TCTTAGTATCTCTCTCTGTTTTGCTGCTTGTGCACTTTTCTATTTCTACCGGGGTGCCAGCGCTGACGCA GAACCTACCAAGGATACTCAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCTGCTGGAGAT GGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAATTTCAAGAAT TAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGC TACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGAT AATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGG TTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAAT CATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACC AAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCT CGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGAGAATCTCAT ACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTAATAGCCAAA GTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAGCCAAGCTGA AGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGAAGATGCAAG GCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACCAGAGTAGAA ATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAGCTGTTCGAT GGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAGCATCTGGCA ATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTTAACCTGAAG TACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTCAGCTCCCTG GGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCGCAGAGAGCA AAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAAGCTTTCGGC TGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAAATTTACCTG TGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGTAGGAGTCAA TTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAGGATATTGCT GGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGGATCAAGAAC TCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTGTACCAGCAC CCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGTTTCACTGTT TGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTGCAGGAGAAG AACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAGCTCCGCAGA GATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGAGCTGCCACT TCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAAATAGCGAAG TTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTCCACTGAAGA CACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGAGTTCAGATC CCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAATATCAGTAT TGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAACCATGTGGCT TGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATT AAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGGCGGATGGCA CCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTTAGACCTTGG ACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAGGAACAAA ATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGAAGGAGAGCC AAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCACGCTCCGCCT CTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAAATGTTAGGT ACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTTTGTGGGTGG GGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACCGTAATCCTCCACCAGGGACGTTTACT GGGGCTAAGGGCAAATAAAACCTCAGGGACTCCAGGTAACCGTCCTGGAAGTGTAATCCGTGTATATGGT GATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATACCCTACTGTTCAATGGCACATGCACAGCTTT GTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAGTCCCAGGTCAGGTCATTAGCCCACAAAG TAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGGGTCATCTGCACAGGAGCCCAGTAGCCAGACG CCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGCTACATTGACTTCCGGATGGGTGATGAAGGTG GAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAACCTTCAGTCACCAAAGCCGAGAGGAGTCACCT GATAGTGTGGCAAGTGATGTGTGGCAATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199205
Insert Size	3534 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199205.1, NP_001186134.1
RefSeq Size	6940 bp
RefSeq ORF	3534 bp
Locus ID	70834
UniProt ID	Q58A65
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) has a different 5' terminus and has multiple differences in the coding region, compared to variant 5, which results in a shorter isoform (7) with a different N-terminus, compared to isoform 5.

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