Lamb3 (NM_001277928) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229434 representing NM_001277928 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCATTTTTCCTCCTGTGGCTTGCCTTGCCTGGCTTCCTGTGTGCCCAGCAAGCCTGCTCCCGAG GGGCCTGTTATCCACCTGTGGGGGACTTGCTCATTGGAAGGACTCAGCTTCTTCGAGCCTCATCTACCTG TGGATTGACCAAACCTGAGACCTATTGCACCCAATATGGACAGTGGCAGATGAAATGCTGCAAGTGTGAC TCCAGGCTGCCTCGAAATTACAACAGTCACCGAGTGGAGAATGTCGCATCGTCTTCAGGCCCCATGCGCT GGTGGCAGTCACAGAATGATGTGAGCCCTGTCTCTCTGCAGCTGGACCTAGACAAGAGGATGCAGCTTCA GGACATCATGATGGATTTTAAGGGTCTCACACCAGCTGGCATGTTGATTGAGCGCTCTTCTGACTTTGGC AAGACATGGAGGGTGTACCAGTACCTGGCGACAGACTGTGCCAGCACCTTTCCCCAGGTCCACCAGGGCC AGCCCAAGAACTGGCAGGACGTCCGGTGCCGGCCCTTGTCCCAGAGGCCTAATGGGCATCTGACTGGGGG AAAGGTCCAACTTAACCTTATGGATTTAGCATCAGCTATCCCTGCATCTCAAAGTAAAAAGATTCAAGAA CTTGGGGACATCACAAACTTGAGAGTCAACTTCACCAAGCTGGCCCCTGTGCCCCAGAGGGGCTCCTATC CACCCAGCGCCTACTTTGCAGTGTCACAGCTACGTCTACAGGGGAGTTGCTTCTGTCACGGACATGCCGA CCGCTGTGCCCCTAATCCTGGAGGTTCTACCACTGCTGTGCAGGTCAACAATGTCTGTGTCTGCCAGCAT AACACAGCTGGCCCCAATTGTGACCGCTGTGCCCCTTTCTACAACAACCGTCCATGGAGACCTGCAGAGG GCCAGGACACCCACGAGTGCCAAAGGTGTGACTGCAATGGGCACTCAGAGACCTGCCACTTCGACCCAGC TGTGTTTGCTGCCAGCCAGGGGACAAATGGAGGTGTGTGTGACAACTGCCGGGACCACACCGAGGGCAAG AACTGCGAGCGCTGTCAGCTACACTATTTCCGGAATAGACGACCCAGCGCTCCCATTCATGAGACTTGTA TTCCCTGTGAGTGTGATCCAGATGGAGCAGTCCAGGGAGCTCCCTGTGACCGACTGACGGGCCAGTGTGT GTGCAAAGAGTACGTGCAAGGAGAGCGCTGCGACCTTTGCAAGCCTGGCTTCACCGGGCTCACCTTCGCC AACCCGAAGGGCTGCCATGCCTGTGACTGCAGTATCTTAGGTGCCCGGAAAGATATGCCTTGTGAGGAGG AAACCGGACGCTGCTTGTGTCTGCCCAATGTAGTGGGCCCCAAATGTGACCAGTGTGCCCCTTCCCACTG GAAGCTGGCCAGCGGCCTGGGCTGCGAGCCCTGTGCCTGTGACCCTCGCAACTCCCTAAGCTCCCAGTGC AACCAGTTTACAGGACAGTGTCCTTGTCGGGAAGGGTTTGGCGGCCTCACGTGTAGTTCTGCAGCCATCC GCCAGTGTCCTGACCAGACCTATGGACACGTGGCCACAGGGTGCCGAGCTTGTGACTGTGACTTCAGAGG AACAGAGGGTCCTGGCTGTGACAAAGCCTCAGGCCGTTGCCTCTGCCGCCCTGGCTTTACCGGGCCCCGC TGTGACCAATGCCAACGAGGTCACTGTGACCGCTACCCAGTATGTGTGGCCTGCCACTCCTGCTTCCAGG CCTATGACACAGACCTCCAGGAGCAAGCTCGACGCCTTCACAGTCTCCGTAATGCCACCGAAGGCCTGTG GACAGGGACAGGTCTGGAAGACCATGGCCTGGCTTCTCGGTTGCTAGATGCCAAGAGCAAGATTGAGCAG ATCAGACAGATTCTGGAAGGCACTTCTGTCACAGAGCAGGATGTGGCTCAGGTGGCCAATGGCATCTTGT CTATCAGGAGAACTCTTCAGGGCCTGCCGCTGGACCTGCCCTTAGAGGAGGAGATGGAATCCTTCTCTGG AGACCTGGGGAATCTAGACAGAAGCTTCAGTCGGCTCCTCCTTATGTACCGAAGCAAGAAGGAGCAATTT GAGAAGCTAAGCAGTGAAGACCCTTCAGGAGCCTTCCGCATGCTGACCATGGCTTATGAGCAGTCCTCCC GGGCTGCACAGCAAGTGTCTGACAGTTCTAGCCTGCTGAGCCAGTTGAGGGACAGTCGCAGGGAAGCAGA GGGCCTGGAGAGACAGGCTGGAGGAGGAGGCACCGGAGGAGCTCAGCTCATGGCCCTGCGCCTAGAAATG GCTTCGCTGCCTGACTTGACACCCACCATCAACAAGCTTTGTGGCAGGTCTAGGCAGACAGCCTGTACTC CAGGAGATTGCCCTGGAGAGCTGTGTCCTCAGGACAATGGTACAGCTTGTGGCTCTCACTGCAGGGGAGC CCTGCCCAGAGCCAAAGGGGCCTTCCACATGGCAGGGCGGGTAGCTGAGCAGCTACGAAACTTCAACACC CAGCTCCAGCAGACCAGGCAAATGATCAGGGCAGCCGAGGAAGCAGCATCAAGGGTCCAAGCCGATGCCC AGCGCCTTGAGACCCAGGTGAGTACCAGCCGCTTGCTGATGGAGGAAGATGTCCAGCGCACACGGCTTCT CATCCAACAGGTCCGGGGCTTTCTCACAGATCCTGACACAGATGCAGCCACCATCCAACAGGTCAGCGAG GCAGTGCTGGCTCTCTGGCTGCCCACAGACTCAGCCACAGTGCTGCGCAAGATGAAAGAGATCCAGGCCA TTGCGGCCAGGCTCCCTAATGTGGACTCAGTGCTATCCCAGACCAAACAAGACATCGCACGGGCCCGCAG GCTCCAGGCTGAGGCTGAGCAGGCCAGAAGCCGAGCCCACGCTGTGGAAGGGCAGGTAGATGATGTGGTC GGGAACCTTCGGCAGGGCACCGTGGCTCTGCAGGAAGCTCAGGACACAATGCAGGGTACTGGCCGCTCTC TTCGGCTCATCCAGGAAAGGGTTGGTGAGGTTCAGCAGGTCCTTGTACCAGCTGAAAGGCTGGTGAAAGG CATGAAGGAACAGATGAGTGGATTCTGGGCACGGATGAAGGAGCTTCGCCGTCAGGCCCAGGAGGAGCAG GCCCAGGCAATGCAGGCCCGGCAGCTTGCAGAGGGTGCCAGCCAGCAGGCAATGAATGCCCAGGAGGGCT TTAAGAGACTTAAGCAAAAGTATACAGAGTTGAAGGACCGGCTGGGTCAGAGTCCTGTGCTGGGCGAGCA AGGCAATCGAATCCTGAGCATCAAGATGGAGGCAGAGGAGCTCTTTGGGGAGACCATGGAAATGATGGAC AAAATGAAAGACATGGAGTCAGAGCTGCTTCGAGGGAGTCAGGCTATCATGCTGCGCTCTGCAGACCTGT CGGGGCTGGAGAAGCGTGTGGAGCAGATCCGCAGTTACATCAATGGGCGAGTGCTCTACTATGCCACCTG CAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277928
Insert Size	3507 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277928.1, NP_001264857.1
RefSeq Size	4016 bp
RefSeq ORF	3507 bp
Locus ID	16780
UniProt ID	Q61087
Gene Summary	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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