Fhdc1 (NM_001205355) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330505N24Rik; Gm126
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229415 representing NM_001205355 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGTTATGAATTGTGTCTCCCTGGCCAGCGATAAAGAAAATGGGACTCTTGCCACAGCAGCTGCAT TCATGACTGGGCAGACGTCACCCTCTGCATCTCCTCCACCTCCTCCTCCACCGCCTCCACCTCCACCTTG TCCACATTCAGGGGAGGGCTTTTCCCCCTCCCCTCCTCCTCCTCTGCCACCTCCACTTCCTGGGGGACCT CCCATACCCCCTCCTCCTCCTCCAGGACTCCCCTCAGTTTCTTACCTGAATGGCTACAGCTCCCTGGGTA AGAAGAAACGAATGCGGAGCTTTTTCTGGAAAACCATTCCAGAGGAGCAAGTTCGAGGCAAGACCAACAT CTGGACCTTAGCAGCTAAGCAGCAGCATCAGTACCAGATCGACAAGAAGACCATTGAGGAGCTTTTTGGG CAGCAAGAAGACACCTCCAAGGCTTCTCTTCCCAAGAGAGGAGGAGCTTTGAATTCATCCTTCAGAGATG CCCGGGAAGAGGTTACCGTCTTGGATGCAAAGCGAAGTATGAACATTGGCATATTTCTTAAGCAGTTTAA AAAGTCTCCTCAGTCCATCGTAGAGGATATCTATCAAGGAAAGAGCGAGCATTACGGATCAGAGACGCTT CGAGAAATCCTTAAGCTTTTGCCGGAGTCAGAAGAGGTAAAGAAATTAAAGGCATTTAATGGCGATGTAT CCAAGCTCTCGCTGGCAGATTCCTTTCTGCACTGCTTGATTCAGGTGCCAAACTATTCACTTCGGATTGA AGCCATGGTGCTAAAAAAGGAGTTTTTGCCATCTTGTTCCTCTCTGTTCAAAGACATAAGGACTCTGAGA GCAGCTACAAAAGAGCTGATGTTATGTGAGGAGCTACATTCAATATTACACTTGGTGCTCCAGGCTGGGA ATATCATGAATGCCGGAGGGTATGCCGGCAATGCCGTAGGATTTAAACTGTCTTCTCTACTCAAATTGGC GGACACGAAGGCAAACAAACCTGGAATGAATCTTCTGCATTTTGTAGCTCAGGAAGCCCAGAAGCAAGAT GCCATCCTTCTAAACTTCTCTGAGAAGTTGCAGCACGTGCAAGAGACATCGAGATTATCTCTGGATATCA CCGAGGCAGAGCTGCACTCTCTCTTTGTCAGAACGAAATCGCTGCAGGAAAACATCCAGCTTGACCAGGA GCTGTGCCAGCAGATGGAAGACTTCCTCCAGTTTGCTGTGGAGAAGTTGGCGGAGCTGGAACTCTGGAAA CGGGAGCTGCAGGGTGAAGCTCACACCCTTATAGACTTTTTCTGCGAAGACAAAGAAACCATGAAACTGG ATGAATGCTTCCAGATATTCAGAGACTTCTGTACCAGATTCAACAAAGCAGTTAAGGACAATCATGACCG GGAGGAACAGGAGCGGAAGCAGCTACAGAGGCAGAAGGAGATGGAACAGAAGCGGTATTCCTGGTCAACT GGGGAACTCGGGAGCTTTGGCAGGAGCAGCAGTGAGAATGATGTGCAGATGCTAGCTAAGACAGGCACAG AAGACCTGCCCTCCTTCCTGAAGCCCAGGCCCAACAGCCCCTCCTACCGGCCCCCCAACACACGTCGCTC CCGCCTCTCCCTGGGCATCTCTGCGGACCGGGAGCTTTTGACCTTCCTGGAGAGTGCCACCAGCAGTCCA GAGGACCCCAACAAATTCAACAGTTTGCCCCGGAGCAGTCCCCGACAAGCTCGGCCCACCATCGCCTGGA TGGAACCCAGAGAACAACAGAGCCATGGCCCTAACTTTACACATGAACCTCAGGCCTCAAAGATCCAGGA GAAAGCCCCTCCCCCTGCTTGGCAAAATCAGCTTCCGACAACCTGGCGGGAGGAGCCTGCTTCTCCCCTC CCACTGGCTGGGCGGAGCAGGCCGAGCCTCCGAAAAAGGAACAGTGAACCTGTGGGCTTGGGTCCCACAC AAAGCCCTCCACTCTTACCATTGGATCTGGGTGTTAGGGAGCATGAGTTGGTCACAGGGCTGACCCAGTT TGACCTCCAGAGTCCCAAGAGCCTGGAGGAAGGATCCCAACTGACTCTGAATGACTTCTGTCCCACAAAG CTGCCATCTCCAGGGGATAGGAGCTCACAGCCCTTTGCTGCTGGCGGTGACAGTCTCCCACCCAAGGACA CAGACACCCAGGAGGTTCTCAGCCCAGCTGGGGAGGATGACAGGACCATCTCTGATGAACCCAGCAGTGA GGCTCTCGTGTCTGTGGTTGTCACCGACACTGAAGATAAGGATGCTGGACCTCTGTTATATGTCTCAGAT ACCACAGACTGCTCACTGACTCTGGACTGCTCCGAGGGAATGGACTCCAGAGCCGGGGGAGACAAGCAAG AGGAAGAGAAGGAAGGGGATGGCTCAGTGTCCTCTGGGGCTGGAGAGGCAGGAAGCAGCCAGGTTTCCTC CAACTCTGTTTCCAGCCCCCCTGGGGAGGTTCCTGCTCCAAAATCCAGTAAGAGTGAGCTGAGCTGCCAA GGTGGCCTTCCGAAGGACAGACCCAGCAGAGGGAAGGATGCTATAGCACCAAAGAGAAATTCCTTCAAAG AGGCTTCAGTAGGGGCTTCAAAGCCTGTCAGTGCACGGCGCAGCCAAGGGGTAACAACCAAGCCTGTGCG AACCTTGAACTCCTCCGAGAACGAACATATGCGCAAAGTGGTCCCTATCTCCAAGTCCAGCAGGGGAGCC GGGCCCTGGAAGCGGCCGGAACCGACACCCAAGGCTACCCCTCGGGAGACACCCAGCAGCACAGACACAC CACTATCACGAAGGAGCTCAGTGAGAGGAACTTCGGACACTTCGCCCAGGAGGCCCCAGGTGAGTGGATC TGGGGCAGAGGAGCCCAGGCTGCCCCGCTCGAGTGGCAGCATCAGTGGACGGCCAGGGAAGGACGCCCCG CTGCAGCCCAGAGCCTCGTTCAGGAAGCCTAGCGCCAAGCCCCTCAGAAACATCCCCAGGCAGAAACCTG AAGAAAACAAGGTCAGCAGCCCCAACTCTCCTGACCCCGAGAGTCCCAAGGAAGAGCCCAAGGCTCCTCA GGCTACGGGTGTCTCTCGAGCCCTTCCCCCCATCCCAAGTTTTGCTCGAAATACGGTGGCCTCGTCATCT CGGAGCCTGCGAACGGATGCTCCACCCGCGGCCAGAACCACTGGCCTCACCAGGACAGTCTCCCAGCGGC AGCTTAGGGTCAAAGGTGGCTCCGAGGATTCTGCCTCCAAGGACATTGGCACTTTGAAGAGGGCCAGCAG TGCTCGGGCCTCCAAGAAGTGTCCTGAATCTGCTGGGGGTTCAAGTGCCAATGTAGAAACCTCACTGAAG GGCAGAGGGACGACGGAAAGATCCTCTCTCAGACTGAAGGACTCGGGGCAGGCCACCCTCGGGAGGATCC TCCGTCCCCTGCAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205355
Insert Size	3450 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205355.1, NP_001192284.1
RefSeq Size	6001 bp
RefSeq ORF	3450 bp
Locus ID	229474
UniProt ID	Q3ULZ2
Gene Summary	Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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