Atp11a (NM_001293668) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930558F19Rik; Atpc1h; AU040868; Ih
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229406 representing NM_001293668 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGCAGTCTATTGAGGACGCTCGTGCGCAGATACTGTGCAGGGGAAGAGAACTGGGTGGACAGTC GGACCATCTACGTGGGACACAAGGAGCCCCCTCCGGGTGCAGAGGCCTACATTCCACAGAGATACCCCGA CAATAGAATCGTCTCCTCCAAGTACACATTCTGGAACTTCATACCCAAGAACTTATTTGAACAGTTCAGA AGAATAGCCAACTTTTATTTCCTCATCATCTTCCTGGTACAGTTGATCATCGACACACCCACAAGTCCAG TGACAAGCGGGCTCCCACTCTTCTTCGTCATCACTGTCACGGCCATCAAGCAGGGCTATGAAGACTGGCT TCGCCACAAGGCCGACAACGCCATGAACCAGTGTCCCGTGCACTTTATCCAGCATGGCAAGCTGGTCCGC AAGCAGAGTCGGAAGCTGAGGGTTGGGGACATTGTCATGGTGAAGGAAGATGAGACCTTTCCCTGTGACC TGATCTTTCTCTCCAGCAACCGGGCAGATGGGACATGCCATGTCACTACAGCCAGCTTAGACGGAGAGTC GAGCCATAAAACTCACTACGCAGTGCAGGATACCAAGGGCTTCCACACAGAGGCGGATGTTGACAGCTTG CACGCCACGATCGAGTGTGAACAGCCACAGCCTGACCTCTACAAGTTTGTGGGGCGCATCAATGTTTACA ACGACCTGAACGACCCTGTAGTGAGGCCTTTGGGGTCAGAAAACCTGCTTCTCAGAGGAGCCACACTCAA AAACACAGAGAAGATCTTTGGTGTGGCTATCTACACAGGCATGGAGACCAAGATGGCCCTGAACTATCAA TCCAAGTCCCAAAAGAGATCTGCTGTGGAAAAGTCAATGAACACATTCCTGATCGTGTACCTCTGCATCC TGGTGAGCAAGGCCCTGATCAACACGGTGCTGAAGTACGTGTGGCAGAGTGAGCCCTTCCGAGATGAGCC ATGGTACAACGAGAAGACCGAATCCGAGCGCCAGAGGAATTTGTTCCTCAGGGCCTTCACCGACTTCCTG GCCTTCATGGTCCTCTTCAATTACATCATCCCCGTATCCATGTACGTCACCGTGGAGATGCAGAAGTTCC TCGGCTCCTACTTCATCACCTGGGATGAGGACATGTTTGATGAGGAAATGGGGGAAGGGCCTCTGGTCAA CACATCTGATCTGAATGAGGAGTTGGGACAGGTGGAATACATCTTCACTGACAAGACCGGCACGCTTACA GAGAACAACATGGCCTTCAAGGAGTGCTGCATCGAGGGCCATGTCTACGTACCCCATGTCATCTGCAATG GGCAGGTCCTTCCCGACTCTTCTGGCATTGACATGATCGATTCTTCCCCCGGAGTCTGTGGAAGGGAACG GGAGGAGCTGTTTTTCAGGGCCATCTGCCTGTGCCACACTGTCCAAGTGAAAGATGACCATTGTGGGGAT GATGTCGATGGTCCCCAGAAATCTCCAGATGCAAAATCCTGTGTGTACATATCGTCCTCGCCTGATGAGG TTGCACTGGTCGAAGGCGTGCAGAGGCTTGGATTCACGTACCTGAGGCTGAAGGACAATTACATGGAAAT ACTGAACAGGGAGAACGACATAGAGAGATTTGAATTGCTAGAAGTGCTGACCTTTGATTCTGTGCGGAGA AGAATGAGTGTGATTGTAAAGTCTACTACAGGAGAAATTTATCTGTTTTGCAAAGGAGCAGATTCTTCAA TATTTCCCCGAGTGATAGAAGGCAAAGTAGACCAGGTCCGGTCTAGAGTGGAGCGCAATGCCGTGGAAGG GCTGCGAACCCTGTGTGTTGCATACAAGAGGCTCGAGCCAGAGCAGTACGAAGATGCTTGCCGACTGCTA CAGAGTGCCAAAGTGGCGCTTCAGGACCGGGAGAAGAAGCTGGCAGAAGCCTACGAGCAGATAGAGAAGG ACCTTGTTCTGCTTGGTGCTACGGCTGTTGAGGACAGGCTCCAGGAGAAAGCTGCTGACACAATTGAGGC ACTACAGAAGGCAGGCATCAAGGTCTGGGTGCTCACCGGGGACAAGATGGAGACAGCATCCGCCACCTGC TATGCCTGCAAGCTGTTCCGCAGGAGCACGCAGCTGCTGGAGCTGACCACCAAGAAGCTAGAGGAGCAGA GCCTGCACGATGTGCTCTTCGACCTGAGCAAGACAGTGCTGCGCTGCAGCGGGAGCATGACCAGGGACTC CTTCTCGGGGCTCTCCACAGATATGCACGACTATGGTTTAATCATCGATGGAGCAGCACTGTCCTTGATA ATGAAGCCGAGAGAGGACGGGAGCTCCTCGGGCAACTACCGAGAGCTCTTCCTGGAGATCTGCAGGAACT GCAGTGCTGTGCTGTGCTGCCGGATGGCACCCTTGCAGAAGGCCCAGATCGTTAAGTTAATCAAATTTTC AAAGGAGCATCCTATTACCTTAGCAATTGGTGATGGTGCAAATGATGTCAGTATGATCCTGGAGGCCCAC GTGGGCATAGGTGTCATCGGGAAAGAGGGTCGCCAGGCTGCAAGGAACAGCGACTATGCAATACCCAAGT TTAAACACTTAAAGAAGATGCTTCTTGTTCATGGGCATTTTTATTATATCAGGATATCTGAGCTTGTGCA ATACTTCTTTTATAAGAACGTCTGCTTCATTTTCCCTCAGTTTTTGTACCAGTTCTTCTGCGGATTTTCA CAGCAGACTTTGTACGATACTGCGTATCTGACTCTCTACAACATCAGCTTCACTTCCCTCCCGATTCTCC TGTACAGCCTCATGGAGCAGCACGTGGGCATTGACGTGCTCAAGAGAGACCCGACCCTCTACAGAGACAT TGCCAAGAATGCGCTGCTGCGCTGGCGGGTGTTCATTTACTGGACGTTTCTCGGCGTTTTTGACGCTTTG GTATTTTTCTTTGGTGCTTATTTCATATTTGAGAACACAACCGTGACCATCAACGGACAGATGTTTGGGA ACTGGACCTTCGGGACGCTGGTGTTCACTGTGATGGTGCTCACTGTCACACTGAAGCTCGCACTGGACAC GCACTACTGGACTTGGATCAACCACTTTGTCATCTGGGGGTCGCTGCTCTTCTACATTGCCTTCTCCCTG CTCTGGGGAGGGGTTATCTGGCCGTTCCTCAGTTACCAGAGGATGTACTATGTGTTCATATCCATGCTGT CCAGTGGGCCTGCCTGGCTGGGTATCATACTGCTTGTCACGGTCGGCCTCCTCCCTGACGTCCTCAAGAA GGTCCTGTGTAGGCAGCTGTGGCCCACGGCAACGGAGAGAACTCAGGCCAGGAACAAGTGCCTTTCTGTT GAGCAGCCCACCGTCTTTATGCTTTCCCAGACCGCCAGTAGTCTGAGTTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293668
Insert Size	3414 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293668.1, NP_001280597.1
RefSeq Size	7528 bp
RefSeq ORF	3414 bp
Locus ID	50770
Gene Summary	Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon in the 3' end compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

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