Nckap1 (NM_001290745) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C79304; H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1; p125Nap1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229401 representing NM_001290745 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGCTCCGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG GCGTCGGCATGCTCACGCGCCTCTACAACATCAAGAAGCAAGGACAAGTTTGGAAGGCATGTGGCGACCC CAAGGCTAAACCCTCCTACCTTATTGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTT CCTGCTGTAGAAACGCGCAACAACAATCAACAGCTGGCACAGCTACAGAAAGAAAAATCAGAGATTCTGA AAAATCTGGCGTTGTATTACTTTACATTTGTAGATGTTATGGAATTTAAGGACCATGTCTGTGACTTGCT GAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTGAACTTTGATTTAACAAAGAACTACTTA GACTTGACTGTAACCTATACAACATTAATGATACTGCTGTCCCGGATTGAAGAACGGAAGGCAATCATTG GACTATACAATTATGCACATGAAATGACCCATGGAGCAAGTGACCGAGAATATCCACGCCTTGGTCAGAT GATTGTGGACTATGAAAACCCTTTAAAAAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCA GATGCGCTAATTTCTCTTCAGATGGTGTATCCTAGACGGAACCTTTCAGCAGACCAGTGGAGGAATGCCC AGTTGCTGAGTCTCATCAGTGCACCCAGCACAATGCTTAATCCTGCGCAGTCGGACACTATGCCTTGTGA ATATCTCTCCTTGGATGCAATGGAGAAATGGATCATCTTTGGCTTTATTCTGTGCCATGGGATGCTAAAT ACGGAGGCTACCGCACTGAACCTTTGGAAGCTAGCTCTTCAGAGTAGCTCTTGCCTCTCTCTCTTTCGGG ATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATTCGGGGCTACAATAAACGTAT TAACGACATAAGAGAATGCAAGGAGGCGGCTGTATCCCACGCCGGCTCAATGCACAGAGAAAGACGCAAG TTTTTACGGTCTGCACTAAAAGAACTGGCCACTGTCCTCTCTGACCAGCCTGGCCTGCTTGGTCCCAAGG CACTTTTTGTTTTCATGGCGTTATCTTTTGCCCGTGATGAAATTATCTGGCTGCTCCGTCACGCGGACAA CATGCCAAAGAAGAGTGCAGATGACTTTATAGATAAGCACATTGCTGAGCTAATATTTTACATGGAAGAG CTTAGAGCACATGTCAGGAAGTATGGGCCTGTCATGCAGAGGTACTACGTGCAATACCTCTCTGGCTTTG ATGCTGTTGTCCTCAATGAACTTGTGCAGAACCTGTCTGTTTGCCCTGAGGATGAATCGATCATCATGTC CTCTTTTGTGAACACCATGACCTCCCTAAGTGTGAAGCAAGTTGAAGATGGAGAAGTGTTTGATTTCAGA GGGATGAGATTAGATTGGTTTAGGTTACAGGCGTACACTAGCGTCTCCAAAGCTTCACTTAGCCTTGCAG ATCACAGAGAACTTGGAAAGATGATGAACACAATCATATTCCATACAAAAATGGTAGATTCCTTGGTTGA AATGTTGGTGGAAACATCCGATCTCTCCATATTTTGTTTTTACAGCCGTGCTTTTGAGAAGATGTTTCAG CAGTGCTTGGAGCTGCCCTCTCAGTCAAGATACTCCATTGCCTTTCCTCTGCTCTGCACTCACTTCATGA GCTGCACACATGAACTGTGTCCTGAAGAGCGCCATCATATAGGAGATCGTAGCCTTTCCCTGTGCAATAT GTTCCTGGATGAGATGGCCAAACAAGCTCGAAATCTCATCACCGATATTTGCACAGAACAGTGTACTCTT AGTGACCAGTTATTGCCGAAGCATTGTGCCAAAACCATCAGTCAAGCAGTGAATAAGAAGTCAAAAAAAC AGACGGGCAAGAAAGGGGAACCTGAAAGGGAAAAACCGGGTGTGGAGAGCATGAGGAAAAACAGGCTGGT AGTGACCAACCTTGATAAGTTGCACACTGCACTTTCGGAGTTATGCTTCTCCATAAATTATGTTCCAAAC ATGGCGGTGTGGGAGCACACCTTCACGCCGAGGGAGTATTTGACTTCTCATCTGGAAATCCGGTTCACTA AATCAATTGTTGGAATGACTATGTATAATCAAGCTACACAGGAAATTGCAAAGCCATCAGAGCTTCTAAC AAGCGTAAGAGCATATATGACTGTACTCCAGTCCATAGAGAACTATGTGCAGATTGATATCACCAGAGTA TTTAATAATGTCCTTCTTCAGCAAACACAACACTTAGACAGCCATGGAGAACCAACCATCACGAGTCTGT ATACAAACTGGTACTTGGAAACTTTATTAAGACAAGTCAGCAATGGCCATATAGCTTATTTTCCTGCAAT GAAAGCATTTGTGAATTTACCCACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGATATATCA GAAATGAGATCATTATCAGAGCTCCTAGGCCCATATGGGATGAAGTTCCTGAGTGAGAGCCTTATGTGGC ACATTTCATCACAAGTAGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTTCTAACACAAATGAG GACCAGCTTTGACAAACCAGACCAGATGGCTGCACTCTTTAAAAGATTATCATCTGTTGACAGTGTCTTG AAAAGGATGACGATAATTGGTGTTATTTTATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCC TGTCCTACCACATTCCTTTCCTCGTGAGCTCCATTGAAGATTTCAAGGATCACATTCCTCGGGAAACTGA TATGAAGGTTGCAATGAATGTGTATGAGCTGTCCTCGGCTGCTGGGTTACCATGTGAGATTGATCCTGCC TTAGTCGTAGCTCTTTCCTCACAAAAATCAGAGAACATTAGTCCAGAGGAAGAATACAAGATCGCTTGTC TCCTGATGGTCTTTGTAGCAGTTTCCTTGCCTACACTGGCCAGCAATGTAATGTCACAGTACAGCCCTGC AATAGAAGGACACTGCAACAATATACATTGTTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTT ACAATTCACAAAGGAAGTATTGAAGACCGACTTAAGGAATTCCTGGCGCTTGCATCATCCAGTCTACTGA AAATTGGCCAAGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTGCTACTGGATATGAT TGTACAAGAATCACCATTCCTGACAATGGATCTCTTGGAATCTTGTTTTCCTTATGTGTTGCTGAGAAAT GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290745
Insert Size	3405 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290745.1, NP_001277674.1
RefSeq Size	4421 bp
RefSeq ORF	3405 bp
Locus ID	50884
Gene Summary	Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

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