Jak2 (NM_008413) Mouse Untagged Clone

CAT#: MC229400

Jak2 (untagged) - Mouse Janus kinase 2 (Jak2), transcript variant 1



  "NM_008413" in other vectors (1)

CNY 7,888.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Fd17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229400 representing NM_008413
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATGGCCTGCCTTACAATGACAGAAATGGAGGCAACCTCCACATCTCCTGTACATCAGAATGGTG
ATATTCCTGGAAGTGCTAATTCTGTGAAGCAGATAGAGCCAGTCCTTCAAGTGTATCTGTACCATTCTCT
TGGGCAAGCTGAAGGAGAGTATCTGAAGTTTCCAAGTGGAGAGTATGTTGCAGAAGAAATTTGTGTGGCT
GCTTCTAAAGCTTGTGGTATTACGCCTGTGTATCATAATATGTTTGCGTTAATGAGTGAAACCGAAAGGA
TCTGGTACCCACCCAATCATGTCTTCCACATAGACGAGTCAACCAGGCATGACATACTCTACAGGATAAG
GTTCTACTTCCCTCATTGGTACTGTAGTGGCAGCAGCAGAACCTACAGATACGGAGTGTCCCGTGGGGCT
GAAGCTCCTCTGCTTGATGACTTTGTCATGTCTTACCTTTTTGCTCAGTGGCGGCATGATTTTGTTCACG
GATGGATAAAAGTACCTGTGACTCATGAAACTCAGGAAGAGTGTCTTGGGATGGCGGTGTTAGACATGAT
GAGAATAGCTAAGGAGAAAGACCAGACTCCACTGGCTGTCTATAACTCTGTCAGCTACAAGACATTCTTA
CCAAAGTGCGTTCGAGCGAAGATCCAAGACTATCACATTTTAACCCGGAAGCGAATCAGGTACAGATTTC
GCAGATTCATTCAGCAATTCAGTCAATGTAAAGCCACTGCCAGGAACCTAAAACTTAAGTATCTTATAAA
CCTGGAAACCCTGCAGTCTGCCTTCTACACAGAACAGTTTGAAGTAAAAGAATCTGCAAGAGGTCCTTCA
GGTGAGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGGTCAAGAGGGAAACATA
AGGAAAGTGAGACACTGACAGAACAGGACGTACAGTTATATTGTGATTTCCCTGATATTATTGATGTCAG
TATTAAGCAAGCAAACCAGGAATGCTCAAATGAAAGTAGAATTGTAACTGTCCATAAACAAGATGGTAAA
GTTTTGGAGATAGAACTTAGCTCATTAAAAGAAGCCTTGTCATTCGTGTCATTAATTGACGGGTATTACA
GACTAACTGCGGATGCGCACCATTACCTCTGCAAAGAGGTGGCTCCCCCAGCTGTGCTCGAGAACATACA
CAGCAACTGCCACGGCCCAATATCAATGGATTTTGCCATTAGCAAACTAAAGAAGGCGGGTAACCAGACT
GGACTATATGTGCTACGATGCAGCCCTAAGGACTTCAACAAATACTTTCTGACCTTTGCTGTTGAGCGAG
AAAATGTCATTGAATATAAACACTGTTTGATTACGAAGAATGAGAATGGAGAATACAACCTCAGCGGGAC
TAAGAGGAACTTCAGTAACCTTAAGGACCTTTTGAATTGCTACCAGATGGAAACTGTGCGCTCAGACAGT
ATCATCTTCCAGTTTACCAAATGCTGCCCCCCAAAGCCAAAAGATAAATCAAACCTTCTCGTCTTCAGAA
CAAATGGTATTTCTGATGTTCAGATCTCACCAACATTACAGAGGCATAATAATGTGAATCAAATGGTGTT
TCACAAAATCAGGAATGAAGATTTAATATTTAATGAAAGTCTTGGCCAAGGTACTTTTACAAAAATTTTT
AAAGGTGTAAGAAGAGAAGTTGGAGATTATGGTCAACTGCACAAAACGGAAGTTCTTTTGAAAGTCCTAG
ATAAAGCACATAGGAACTATTCAGAGTCTTTCTTCGAAGCAGCAAGCATGATGAGTCAGCTTTCTCACAA
GCATTTGGTTTTGAATTATGGTGTCTGTGTCTGTGGAGAGGAGAACATTCTGGTTCAAGAATTTGTAAAA
TTTGGATCACTGGATACATACCTGAAGAAGAACAAAAATTCCATAAATATATTATGGAAACTTGGAGTGG
CTAAGCAGTTGGCATGGGCCATGCATTTTCTAGAAGAAAAATCCCTTATTCATGGGAATGTGTGTGCTAA
AAATATCCTGCTTATCAGAGAAGAAGACAGGAGAACGGGGAACCCACCTTTCATCAAACTTAGTGATCCT
GGCATTAGCATTACAGTTCTACCGAAGGACATTCTTCAGGAGAGAATACCATGGGTACCTCCTGAATGCA
TTGAGAATCCTAAAAATCTCAATCTGGCAACAGACAAGTGGAGCTTCGGGACCACTCTGTGGGAGATCTG
CAGTGGAGGAGATAAGCCCCTGAGTGCTCTGGATTCTCAAAGAAAGCTGCAGTTCTATGAAGATAAGCAT
CAGCTTCCTGCACCCAAGTGGACAGAGTTAGCAAACCTTATAAATAATTGCATGGACTATGAGCCAGATT
TCAGGCCTGCTTTCAGAGCTGTCATCCGTGATCTTAACAGCCTGTTTACTCCAGATTATGAACTACTAAC
AGAAAATGACATGCTACCAAACATGAGAATAGGTGCCCTAGGGTTTTCTGGTGCTTTTGAAGACAGGGAC
CCTACACAGTTTGAAGAGAGACACTTGAAGTTTCTACAGCAGCTTGGCAAAGGTAACTTCGGGAGTGTGG
AGATGTGCCGCTATGACCCGCTGCAGGACAACACTGGCGAGGTGGTCGCTGTGAAGAAACTCCAGCACAG
CACTGAAGAGCACCTCCGAGACTTTGAGAGGGAGATCGAGATCCTGAAATCCTTGCAGCATGACAACATC
GTCAAGTACAAGGGAGTGTGCTACAGTGCGGGTCGGCGCAACCTAAGATTAATTATGGAATATTTACCAT
ATGGAAGTTTACGAGACTATCTCCAAAAACATAAAGAACGGATAGATCACAAAAAACTTCTTCAATACAC
ATCTCAGATATGCAAGGGCATGGAATATCTTGGTACAAAAAGGTATATCCACAGGGACCTGGCAACAAGG
AACATATTGGTGGAAAATGAGAACAGGGTTAAAATAGGAGACTTCGGATTAACCAAAGTCTTGCCGCAGG
ACAAAGAATACTACAAAGTAAAGGAGCCAGGGGAAAGCCCCATATTCTGGTACGCACCTGAATCCTTGAC
GGAGAGCAAGTTTTCTGTGGCCTCAGATGTGTGGAGCTTTGGAGTGGTTCTATACGAACTTTTCACATAC
ATCGAGAAGAGTAAAAGTCCACCCGTGGAATTTATGCGAATGATTGGCAATGATAAACAAGGGCAAATGA
TTGTGTTCCATTTGATAGAGCTACTGAAGAGCAACGGAAGATTGCCAAGGCCAGAAGGATGCCCAGATGA
GATTTATGTGATCATGACAGAGTGCTGGAACAACAATGTGAGCCAGCGTCCCTCCTTCAGGGACCTTTCC
CTTCGGGTGGATCAAATCCGGGACAGTATAGCTGCGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008413
Insert Size 3399 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008413.3, NP_032439.2
RefSeq Size 5055 bp
RefSeq ORF 3399 bp
Locus ID 16452
UniProt ID Q62120
Gene Summary Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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