Adcy9 (NM_001291910) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AC9; ACtp10; AW125421; D16Wsu65e; mKIAA0520
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229381 representing NM_001291910 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTACCTCTGTACCTGAGCTTGTTTTTGGGGGTGGTCTATTCTGTCCTTTTTGAGACCTTCGGCT ACCACTTCCGAAACGAAGACTGCTACCCTTCTCCGGGCCCTGGGGCCCTGCACTGGGAGCTGCTGAGCAG AGCCCTGCTTCACGTGTGCATTCACGCTATCGGGATCCATCTGTTTGTCATGTCTCAGGTGAGGTCCAGG AGCACCTTTCTCAAGGTGGGACAATCCATTATGCACGGCAAAGATCTGGAAGTAGAGAAAGCCCTGAAAG AGAGGATGATTCATTCAGTGATGCCAAGAATCATAGCCGACGACTTAATGAAACAAGGGGACGAGGAGAG TGAGAATTCCGTCAAGAGGCATGCCACCTCCAGTCCCAAGAACAGGAAGAAGAAGTCCTCCATACAGAAG GCACCGATAGCATTCCGCCCCTTTAAGATGCAGCAGATTGAAGAAGTCAGTATTTTATTTGCAGACATTG TGGGTTTCACCAAGATGAGCGCCAACAAATCTGCGCATGCCTTGGTAGGCCTACTCAATGACCTGTTCGG TCGCTTTGACCGCCTGTGTGAGCAGACCAAGTGTGAGAAGATCAGCACTCTGGGGGACTGTTATTACTGT GTGGCAGGGTGTCCGGAGCCCCGGGCAGACCATGCCTACTGCTGCATTGAAATGGGCTTAGGCATGATAA AAGCCATCGAGCAGTTCTGCCAGGAGAAGAAAGAGATGGTGAACATGCGTGTTGGGGTTCACACGGGGAC TGTCCTGTGTGGCATCCTGGGCATGAGGAGGTTTAAATTTGATGTGTGGTCCAACGATGTGAACTTGGCT AATCTCATGGAGCAGCTGGGAGTGGCTGGCAAAGTTCACATATCTGAGGCCACTGCAAAATACTTAGACG ACAGGTATGAAATGGAAGATGGGAGAGTTATTGAGCGCCTTGGGCAGAGTGTGGTGGCTGACCAGTTGAA AGGTTTGAAGACATACCTGATATCGGGTCAGAGAGCCAAGGAGTCCCACTGCAGCTGTGCAGAGGCCCTG CTTTCTGGCTTTGAGGTCATTGACGACTCACGGGAGTCCTCAGGCCCTAGGGGACAGGGGACAGCATCGC CAGGGAGTGTCAGTGATTTGGCGCAGACTGTCAAAACCTTTGATAACCTTAAGACTTGCCCTTCTTGTGG AATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCTGTCAAGACGAG CCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAACTCCAAAACCCAGAATGGACTTCTGAGCCCTCCTG CAGAGGAGAAGCTCACTAACAGCCAGACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGACGGTGGGCAGG GGTGAGCTTGGACCAGTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAACTGATGCCCAC TTTGTTGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCA GCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAGGTCATAAAGAA TTCTCCTGTGAAGACGTTCGCCAGTGCCACCTTCAGCTCCCTTCTGGATGTGTTTCTGTCAACCACCGTG TTCTTGATTCTCTCCATCACCTGCTTCCTAAAGTATGGAGCCACCGCCACCCCTCCCCCACCGGCTGCCC TGGCCGTCTTTGGTGCAGACCTGCTGCTGGAGGTGCTTTCCCTCATAGTGTCCATCAGAATGGTGTTTTT CCTAGAGGATGTCATGACATGCACAAAGTGGTTGCTGGAATGGATCGCTGGCTGGCTCCCTCGCCACTGC ATTGGGGCAATCTTGGTGTCTCTTCCTGCCCTGGCTGTCTATTCACACATCACCTCTGAGTTTGAGACCA ACATACATGTTACCATGTTCACTGGCTCTGCGGTGCTGGTGGCCGTTGTGCACTACTGTAACTTCTGCCA GCTCAGCTCCTGGATGAGGTCCTCCCTTGCCACCATCGTGGGGGCTGGGCTGCTGCTTCTGCTCCACATC TCCCTGTGTCAGGACAGTTCCATTGTGATGTCCCCCTTGGACTCAGCACAGAATTTCAGTGCCCAGAGGA ACCCATGCAACAGCTCAGTGCTGCAGGACGGCAGGAGGCCGGCCAGCCTCATAGGCAAGGAGCTTATCCT CACCTTCTTCCTCCTGCTCCTCTTGGTCTGGTTCCTGAACCGGGAGTTCGAGGTCAGCTACCGGCTGCAC TACCATGGGGATGTGGAGGCCGACCTACACCGCACCAAGATCCAGAGCATGAGAGACCAGGCTGACTGGC TACTGCGGAACATCATCCCCTACCATGTGGCTGAGCAGCTCAAGGTCTCTCAGACCTACTCCAAGAACCA TGACAGCGGGGGAGTCATCTTTGCCAGCATTGTCAACTTCAGTGAATTCTATGAGGAGAACTATGAGGGG GGCAAGGAGTGCTACCGTGTCCTCAACGAGCTGATCGGTGACTTCGATGAGCTCTTGAGCAAGCCGGACT ATAATAGCATCGAGAAGATCAAGACCATCGGGGCCACATACATGGCAGCCTCAGGGCTGAACACGGCCCA GTGTCAGGAGGGTGGCCACCCACAGGAGCATCTGCGTATCCTCTTCGAGTTCGCCAAGGAGATGATGCGC GTGGTGGATGACTTCAACAACAATATGTTATGGTTCAACTTCAAGCTCAGGGTCGGCTTTAACCACGGAC CCCTCACAGCAGGTGTCATAGGTACCACCAAGCTGCTGTATGACATCTGGGGGGACACCGTCAACATCGC CAGCAGGATGGACACCACTGGTGTGGAGTGCCGTATCCAGGTGAGCGAAGAGAGCTACCGTGTGCTGAGC AAGATGGGTTATGACTTTGACTACCGAGGGACCGTGAATGTCAAGGGGAAAGGGCAGATGAAGACCTACC TTTACCCAAAGTGCACGGACAATGGAGTGGTTCCCCAGCACCAGCTGTCCATCTCCCCAGACATCCGAGT CCAGGTGGACGGCAGCATTGGGCGGTCTCCCACAGATGAGATTGCCAACTTGGTGCCTTCCGTTCAGTAT TCGGACAAGGCTTCCCTGGGATCTGATGATAGCACACAGGCTAAGGAAGCTCGCCTGTCCTCTAAGAGGT CCTGGAGAGAGCCAGTCAAAGCAGAGGAAAGGTTTCCATTTGGCAAAGCCATAGAAAAGGACAGCTGTGA AGACATAGGAGTAGAAGAGGCCAGTGAACTCAGCAAGCTCAATGTCTCAAAGAGTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291910
Insert Size	3351 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291910.1, NP_001278839.1
RefSeq Size	7632 bp
RefSeq ORF	3351 bp
Locus ID	11515
Gene Summary	Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors. Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and by beta-adrenergic receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2), has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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