Asap1 (NM_001276463) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229377 representing NM_001276463 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT CCGAAAGAAGACTCTCAGAGCCGGCAAGGTGGGTACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACG GCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTG TGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTC CTCACCTGCCAAGTGAAACCGAATGCTGAGGACAAGAAGTCTTTTGACCTGATATCACATAATCGAACAT ATCACTTTCAAGCAGAAGATGAGCAGGATTATATAGCGTGGATATCAGTACTGACGAATAGCAAAGAAGA GGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCC ATCATCGAGGATGTACAGCGGCTCCCTGGGAATGACATCTGCTGTGACTGCGGCTCATCAGAACCCACGT GGCTTTCAACCAACTTGGGTATTTTGACCTGTATAGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCA TATTTCTCGCATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTA GGAAACAATAGTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAA GTGATATGACCGTGCGTAAAGAGTACATCACTGCAAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTG TGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTGAGGCCATCAAGTCCAGGGACTTACTTGCATTAATC CAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCCACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAG CCCTTCATCTTGCCGTCAGGACCGCAGACCAGACATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTG TGGGAACCTGGATAAGCAAACGTCTGTGGGGAATACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCG GAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCACCGTGGACATCGTTAACCAGAATGGAGAAACTGCCT TGGATATAGCAAAGAGACTCAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTT CAACCCTCATGTCCACGTAGAGTATGAGTGGAATCTTCGACAGGACGAGATGGATGAGAGCGATGACGAT CTGGATGACAAGCCAAGCCCGATCAAAAAGGAACGCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCA GCATCTCGCCACAGGACAAGCTGGCACTGCCAGGGTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTA CGGAGCCTTCACCAACCAGATCTTCGCCTCTACGAGCACAGATTTGCCCACATCACCCACCAGTGAGGCT CCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAGGCACCC AGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCAGGACACAAGAGAAC CCTGTCTGACCCTCCCAGCCCACTACCTCACGGGCCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGAT GTGGGCCCATTATCTTCAAGTAAGACGGCCAACAAGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTT CTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGA GACCAGCCATCATCTCTCCCTCGACAGAACCAACATCCCACCTGAGACTTTTCAGAAATCATCACAGTTG ACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCTGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCC AAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAA GCCACAGCTCTCAGACTTACCTCCCAAGCCACAGATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGAT CTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGCCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGT CACACACCGGGGATCTGTCTCCAAATGTACAGTCCAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTC CAACGACCTCACACCCACGCTGCCAGAGACACCCGTACCACTGCCGAGAAAAATCAATACGGGGAAAAAT AAAGTGAGGCGGGTGAAGACCATTTATGACTGCCAGGCAGATAATGACGACGAACTCACATTTATTGAGG GGGAAGTGATCATTGTCACCGGGGAAGAGGACCAGGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGA AAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACATCCTGTCTGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276463
Insert Size	3339 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276463.1, NP_001263392.1
RefSeq Size	6145 bp
RefSeq ORF	3339 bp
Locus ID	13196
Gene Summary	May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream start codon, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a.

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