Rbl2 (NM_001282000) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	p130; PRB2; Rb2; RBR-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229363 representing NM_001282000 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTGGAGGCAACCAGTCGCCACCGCCTCCTCCAGCTGCTGCAGCCAGCTCGGAGGAAGAGGAGG AGGATGGCGACGCCGCGGATCGCGCGCAGCCCGCGGGGTCCCCGAGCCATCAGATCCAGCAGCGGTTCGA GGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGCGCCGAGGCCTGGAGCAGCTACCGCAGCATG AGCGAGAGCTACACGCTGGAGGGAAATGACCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAA AATCTGTTCCAACTGTGAGCAAAGGGACCGCTGAAGGAAACTATGTATCTTTAACCAGAATCCTTCGCTG TTCGGAGCAGAGCCTAATTGAATTTTTTAACAAGATGAAGAAGTGGGAAGACATGGCAAATCTGCCCCCA CATTTCCGAGAACGTACTGAAAGATTAGAAAGAAACTTCACTGTTTCTGCTGTGATTTTTAAGAAATATG AACCCATTTTTCAAGACATTTTTAAATATCCCCAAGAAGAACAGCCTCGCCAGCAAAGAGGAAGAAAACA GAGGCGACAGCCCTGTACCACATCAGAAATTTTCCATTTTTGCTGGGTGCTTTTTATATATGCGAAAGGC CTGTCCGAGGACTGTCACCCCAAGGACTCTAAAGCGTCCTCCGACCCGCCGTGTGTCATTGAGAAGCTGT GCTCCTTACACGACGGTCTAGTGTTGGAGGCCAAGGGGATAAAGGAACACTTCTGGAAACCCTATATTAG GAAACTGTTTGAGAAAAAGCTTCTCAAGGGGAAGGAAGAAAATCTTACTGGCTTCCTGGAGCCCGGAAAC TTTGGAGAGAGTTTTAAGGCCGTTAATAAGGCATATGAAGAATACGTGTTAGCCGCTGGGAATCTGGATG AGCGCGTATTCCTTGGTGAGGATGCTGAGGAGGAAGTTGGGACTCTGTCTCGGTGTCTAAGTGCTGCCTC AGGTACAGAGAGTGCTGAACGGACGCAGATGAGAGACATCTTGCAGCAGCATCTTGACAAGTCTAAAGCA CTTAGAGTCTGCACACCACTGACTGGCGTGAGGTATGTTCAGGAGAACAGCCCGTGTGTGACTCCCGTCT CCACAGCTGCACACAGCCTGAGCCGTCTTCACACCATGCTGTCCGGCCTCAGGAATGCACCCAGTGAGAA GCTGGAGCGGATACTCAGGTCATGTTCCCGAGATCCAACTCAGGCTATCGCTGACAGATTGAAAGAAATG TACGAAATATATTCTCAGCATTTCCAGCCAGATGAGAATTTTAGTAATTGTGCTAAAGAAATTGCCAACA AACATTTTCGTTTTGCAGAAATGCTCTACTATAAAGTATTAGAGTCTGTTATTGAGCAAGAACAAAAAAG ATTGGGAGACATGGATTTATCTGGTGTTCTGGAGCATGACGCATTCCACAGGTCACTCTTGGCCTGCTGC CTTGAGGTGGTCGCTTTTTCCCATAAGCCTCCTGGGAATTTTCCATTTATTGCTGAAATATTTGATGTAC CACATTATCATTTTTATAAGGTAATTGAAGTATTTATTAGAGCAGAAGACGGTCTTTGCAGAGAAGTGGT CAAACACCTCAATCAGATTGAAGAACAAATTTTAGACCATTTGGCATGGAAAACCAAGTCCCCACTGTGG GACAGAATTAGAGATAATGAAAACAGAGTCCCTACTTGTGAAGAGGTCATGCCACCTCAAAACCTAGAGA GAACAGATGAAATTTACATCGCTGGCTCTCCCTTAACCCCGAGAAGGGTGGGTGAAGTTCGTGCTGATGC TGGAGGACTTGGAAGAAGTATAACGTCTCCAACCACATTGTATGACAGGTACAGCTCCCCAACAGTCAGC ACTACTAGAAGGCGGCTATTCGAGAATGATAGTCCCTCTGAAGGAAGCACATCTGGGCGCATCCCCCCAC AACCCCTAGTCAACGCTGTCCCCGTGCAGAATGTACCTGGGGAGACTGTTTCTGTCACACCAGTTCCTGG ACAGACCTTGGTCACCATGGCAACAGCCACTGTCACGGCCAACAATGGACAAACAGTGACCATTCCAGTC CAAGGTATTGCCAACGAAAATGGAGGGATAACCTTCTTCCCAGTCCAAGTCAACGTTGGGGGCCAGGCCC AGGCTGTCGCTGGCTCTATCCAGCCCCTCAGTGCTCAAGCACTGGCTGGAAGTCTGAGTTCCCAACAGGT GACAGGAACCACTTTGCAAGTCCCTGGTCCGGTGGCCATTCAACAGATTTCCCCTGGTGGACAACAGCAG AACCCAGGCCAGCCACTAACCAGCAGCAGTATCCGGCCGCGGAAGACTAGCTCCTTAGCGCTCTTCTTTA GAAAGGTTTACTACTTAGCCGGTGTCCGCCTTCGAGATCTTTGTATAAAACTAGATATTTCAGATGAACT GAGGAAAAAAATTTGGACCTGCTTTGAATTCTCTATAATCCAGTGCACCGAACTTATGATGGACAGACAT CTGGACCAGCTGTTGATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAAGAAGACAGGTCCTTCCAGA ACATCATGCGTTGTTACAGAACTCAGCCACAGGCCCGGAGCCAGGTGTACAGAAGTGTCTTGATAAAAGG GAAAAGAAGAAACTCTGGCAGCAGTGAGAGCAGAAGCCATCAGAATTCTCCAACCGAACTAAATACAGAC AGAGCCAGTAGAGATTCCAGCCCAGTGATGAGGTCAAACAGCACCCTACCAGTTCCACAGCCCAGCAGTG CCCCTCCTACACCAACTCGACTCACGGGTGCCAGCAGTGACGTTGAAGAGGAGGAACGAGGAGACCTCAT TCAGTTCTACAACAACATCTATAGGAAGCAAATCCAAGCGTTTGCCATGAAGTACTCGCAGGCAAACGCG CAGACGGACACTCCTCCCCTCTCTCCCTATCCATTTGTAAGAACAGGCTCCCCTCGCCGAGTACAGTTAT CTCAAAGTCATCCTATCTACATTTCCCCACATAACAACGAAGCAATGCCTTCTCCTCGAGAGAAGATTTT TTACTACTTCAGCAACAGCCCATCAAAGAGACTGAGGGAAATCAACAGTATGATACGGACAGGAGAGACT CCAACTAAAAAGAGAGGGATTCTCTTGGACGACGGAAGTGAATCACCTGCAAAAAGAATCTGCCCAGAGA ATCACTCTGCTCTGTTACGTCGTCTCCAGGATGTGGCGAATGACCGAGGTTCACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282000
Insert Size	3279 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282000.1, NP_001268929.1
RefSeq Size	4806 bp
RefSeq ORF	3279 bp
Locus ID	19651
UniProt ID	Q64700
Gene Summary	Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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