Usp8 (NM_001252580) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI574262; AW557536; mKIAA0055; Ubpy
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229360 representing NM_001252580 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTGTAGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTCAATAAGAAGA CCGAAGTTAAACCTGAGAAAACCAGCACCAAGAATACCAAGTTTTGTAAATTCTCTTCGTTTCTAAGTTA TATACACAGCGCGCAGAAGATCTTCAAGACAGCAGAAGAATGCAGACTAGATCGTGATGAGGAAAGGGCC TATGTGCTTTATATGAAATATGTGGCAGTTTATAATCTTATCAAAAAGAGACCTGATTTCAAGCAACAGC AGGATTATTATCTTTCAATACTTGGACCTGCAAACATCAAAAAAGCTATTGAAGAAGCTGAAAGACTCTC CGAAAGCCTTAAACTAAGATACGAAGAGGCTGAAGTTCGGAAACAACTTGAAGAAAAGGACAGACGGGAG GAAGAGCAGCTGCAGCAACAGAAAAGGCAGGAGATGGGGAGAGAGGACAGCGGTGCGGCAGCCAAACGCT CTGTGGAAAATTTACTGGATTCCAAAACCAAAACCCAAAGGATTAACGGCGAGAAGAGTGAAGGAGCTGC AGCTGCAGAGAGAGGAGCCATCACAGCAAAGGAACTATATACGATGATGATGGATAAAAACACAAGCTTA ATTATAATGGATGCTCGAAAAATACAGGATTATCAGCATTCCTGTATCTTGGATTCTCTCAGTGTTCCTG AAGAAGCTATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCAAACCTCTCAGATGATTCTAAAGACAC ATGGAAAAAGAGGGGGAGTGTGGACTATGTGGTCCTTCTCGACTGGTTTAGTTCAGCGAAAGATTTGCTG CTTGGGACCACTCTACGGAGTCTGAAAGATGCACTTTTCAAGTGGGAAAGTAAAACTGTCCTGCGCCATG AGCCTTTGGTGTTGGAGGGCGGCTATGAAAACTGGCTGCTTTGCTACCCGCAGTTTACAACCAATGCTAA GGTCACTCCACCCCCTCGGAGCAGAGCTGAAGAGGTGTCTGTCTCATTGGATTTTACTTATCCCTCATTG GAAGAACCAGTTCCTTCCAAACTTCCTACCCAGATGCCACCTCCTCCTATAGAAACAAATGAAAAGGCAC TGTTGGTAACTGATCAAGATGAAAAGCTGAGACTGTCAACCCAGCCAGCTCTAGCTGGACCTGGTGCGGC TCCCAGAGCTGAAGCCTCACCCATAATTCAGCCAGCGCCTGCTACAAAGAGTGTTCCACAGGTTGATCGT ACGAAAAAACCGTCAGTCAAGTTGCCTGAAGATCATAGAATAAAATCTGAAAATACAGATCAGAGTGGAA GAGTTCTTTCTGATCGATCCACCAAGCCAGTATTTCCCTCTCCAACCACCATGTTAACAGATGAAGAAAA GGCTCGTATTCATCAAGAAACTGCCCTTCTTATGGAAAAGAATAAACAGGAGAAGGAACTTTGGGACAAG CAGCAGAAGGAACAGAAAGAGAAGCTGAGAAGGGAGGAACAAGAGCGCAAAGCTGGAAAGACACAGGATG CAGATGAACGTGACTCCACTGAGAATCAGCACAAAGCAAAGGATGGACAAGAGAAAAAAGACAGCAAACA GACCAAGACGGAAGACAGAGAGCTTTCAGCAGACGGGGCCCAGGAAGCCACAGGAACACAAAGACAAAGT AAGAGTGAGCATGAAGCTTCTGATGCTAAGGTACCTGTGGAAGGTAAAAGGTGTCCCACGTCAGAGGCGC AGAAGAGGCCAGCAGATGTGTCCCCTGCATCCGTGTCAGGAGAGCTGAATGCAGGCAAGGCTCAGCGAGA ACCTTTGACGAGAGCACGAAGTGAAGAAATGGGGAGAATTGTGCCGGGACTGCCTTTGGGCTGGGCCAAG TTTCTTGATCCAATCACCGGGACCTTTCGTTACTACCATTCCCCCACAAACACAGTTCATATGTATCCAC CTGAAATGGCTCCTTCGTCTGCACCTCCTTCCACCCCGCCCACTCACAAAGTCAAGCCCCAGGTCCCTGC CGAGCGGGACAGGGAGCCATCGAAACTGAAGCGCTCCTACTCCTCACCAGATATCACTCAGGCCCTGCAG GAGGAGGAGAAGAGGAGGCCGGCAGTGACCCCGATGGTCAACCGGGAAAACAAGCCACCATGTTACCCTA AAGCTGAGATTTCGAGGCTTTCTGCTTCTCAGATTCGGAACCTCAATCCTGTATTTGGAGGATCAGGACC AGCTCTTACTGGACTTCGTAATTTGGGAAATACTTGTTACATGAACTCAATCTTGCAGTGCCTGTGTAAT GCTCCACATCTGGCTGATTATTTCAACCGAAACTGCTACCAGGATGATATCAACAGGTCAAATTTGTTGG GGCATAAAGGTGAAGTGGCAGAAGAATTTGGTATAATCATGAAAGCACTGTGGACAGGACAGTATAGATA CATCAGTCCAAAGGACTTTAAAGTCACCATTGGTAAGATTAATGACCAGTTTGCAGGCTCCAGCCAACAG GATTCACAAGAGCTGCTTCTGTTCCTCATGGATGGCCTGCATGAGGATCTGAATAAGGCTGACAATCGGA AGAGGCACAAGGAAGAGAACAACGAGCACCTGGATGACCTGCAGGCGGCCGAGCACGCCTGGCAGAAGCA CAAGCAGCTCAACGAGTCCATCATCGTGGCCCTGTTCCAGGGCCAGTTCAAGTCCACAGTGCAGTGCCTC ACCTGCCGCAGGAGGTCGCGCACCTTCGAGGCCTTCATGTACTTGTCTTTGCCGCTAGCATCCACAAGTA AATGTACTCTACAGGACTGCCTTAGATTATTTTCCAAAGAAGAAAAGCTTACAGATAACAACAGATTTTA CTGCAGCCATTGCCGAGCTCGGCGGGATTCTCTAAAGAAAATAGAAATCTGGAAATTACCTCCTGTGCTG TTAGTGCACCTGAAACGATTTTCCTATGACGGCAGGTGGAAGCAGAAGCTGCAAACATCCGTGGATTTCC CATTGGAAAATCTTGACCTGTCACAGTACGTTATTGGTCCAAAAAACAGCTTGAAGAAATATAACTTGTT TTCTGTTTCAAACCACTACGGCGGGCTAGACGGAGGCCACTACACAGCCTACTGTAAGAACGCGGCAAGG CAGCGCTGGTTTAAGTTTGATGACCATGAAGTTTCTGATATCTCTGTGTCTTCTGTGAGGTCATCAGCAG CTTATATCCTCTTTTATACTTCCCTGGGACCACGCATAACTGATGTAGCCACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252580
Insert Size	3276 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252580.1, NP_001239509.1
RefSeq Size	4210 bp
RefSeq ORF	3276 bp
Locus ID	84092
Gene Summary	Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1 (By similarity). Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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