Pdgfra (NM_011058) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI115593; CD140; CD140a; Pdgfr; Pdgfr-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229357 representing NM_011058 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCTCCCACCAGGTCTTTCTGGTCCTCAGCTGTCTCCTCACAGGGCCGGGCCTCATCTCCTGCC AGCTCTTATTACCCTCTATCCTCCCAAACGAGAATGAGAAGATTGTGCAGCTGAATTCGTCCTTTTCTCT GAGATGCGTTGGGGAGAGTGAAGTGAGCTGGCAGCACCCCATGTCTGAAGAAGACGACCCCAACGTGGAA ATCAGAAGTGAGGAGAACAACAGTGGCCTCTTTGTCACAGTGCTGGAAGTGGTTAACGCCTCCGCAGCCC ACACCGGATGGTACACTTGCTACTACAACCACACTCAGACGGATGAGAGTGAGATCGAAGGCAGGCACAT TTACATCTATGTACCAGACCCAGACATGGCCTTTGTGCCTCTCGGGATGACCGATTCCTTAGTCATCGTG GAAGAGGATGACTCTGCCATCATACCTTGCCGCACCACAGATCCGGAGACTCAAGTAACCTTGCACAATA ACGGGAGGCTGGTGCCTGCCTCCTATGACAGCAGGCAGGGCTTCAACGGAACCTTCAGCGTGGGGCCTTA CATCTGTGAGGCCACCGTCAAAGGGAGGACGTTCAAGACCAGCGAGTTTAATGTTTATGCCTTGAAAGCA ACGTCAGAACTGAATCTGGAGATGGACGCACGCCAGACTGTGTATAAGGCAGGAGAAACGATCGTGGTGA CCTGTGCCGTCTTTAACAACGAGGTCGTTGACCTGCAGTGGACTTACCCTGGAGAAGTGAGAAACAAAGG TATCACCATGCTGGAGGAGATCAAACTCCCATCCATCAAACTGGTGTACACTTTGACCGTCCCCAAGGCC ACGGTGAAGGACAGTGGAGAGTATGAATGTGCTGCCCGTCAGGCCACTAAAGAGGTCAAGGAAATGAAGA GAGTCACCATTTCTGTCCACGAGAAAGGCTTCGTCGAGATTGAGCCCACCTTTGGCCAGCTGGAAGCTGT CAACTTGCACGAAGTCAGAGAGTTCGTGGTGGAGGTGCAGGCCTACCCGACGCCCAGGATATCGTGGCTG AAGGACAACTTGACTCTGATAGAGAATCTCACCGAGATCACCACTGATGTACAGAAGAGTCAGGAAACAA GGTATCAGAGCAAATTAAAGCTGATCCGGGCTAAGGAAGAAGACAGTGGCCATTACACCATTATAGTTCA AAATGAGGATGACGTGAAGAGTTACACGTTTGAGCTGTCAACCCTAGTTCCTGCATCCATTTTGGATCTC GTAGATGACCACCATGGCTCTGGCGGGGGACAGACTGTGAGGTGTACAGCCGAAGGCACCCCTCTTCCAG AAATCGACTGGATGATCTGCAAGCATATTAAGAAATGTAATAATGACACTTCGTGGACAGTTTTGGCCAG CAATGTCTCAAATATTATCACAGAGCTCCCTCGCCGTGGCAGGAGTACCGTGGAGGGACGAGTGTCCTTC GCCAAAGTGGAAGAGACCATCGCAGTTCGATGCCTGGCAAAGAACAACCTCAGCGTTGTGGCCCGTGAGC TGAAGCTGGTGGCTCCCACTCTGCGATCTGAACTCACAGTGGCGGCCGCAGTGTTGGTGCTGTTGGTGAT TGTCATTGTCTCTCTCATCGTCCTGGTGGTCATTTGGAAGCAGAAACCACGGTATGAAATTCGCTGGAGG GTTATCGAGTCAATCAGCCCCGATGGACATGAGTATATCTATGTGGACCCCATGCAGTTGCCTTACGACT CCAGATGGGAATTCCCGAGAGATGGCCTCGTGCTTGGTCGGATTTTGGGATCCGGTGCATTTGGGAAAGT GGTCGAAGGTACAGCTTATGGATTAAGCCGGTCCCAACCTGTAATGAAGGTGGCTGTGAAGATGCTCAAA CCCACAGCCAGGTCTAGTGAAAAGCAGGCTCTCATGTCTGAGCTGAAGATAATGACTCACCTGGGGCCAC ATTTGAACATTGTGAACTTGCTGGGAGCCTGCACCAAGTCAGGTCCCATTTACATCATCACAGAATACTG CTTCTATGGGGATTTGGTCAACTACTTGCATAAGAACAGAGATAGCTTCATGAGCCAACACCCAGAGAAG CCGAAGAAAGACCTGGACATCTTTGGATTGAATCCTGCAGACGAGAGCACAAGAAGTTATGTGATTTTGT CTTTTGAAAATAACGGCGACTACATGGACATGAAGCAAGCTGATACCACACAGTATGTCCCCATGCTTGA AAGGAAAGAGGTTTCTAAATACTCTGACATCCAGAGATCGCTGTACGATCGGCCAGCCTCCTACAAGAAG AAATCCATGCTAGACTCAGAAGTCAAAAACCTCCTTTCGGACGATGACTCCGAGGGTCTGACTTTGCTGG ATCTATTGAGCTTCACCTATCAAGTTGCTCGAGGAATGGAATTTTTGGCTTCGAAAAATTGTGTCCACCG GGACCTGGCTGCCCGCAACGTCCTCCTGGCGCAAGGAAAAATTGTGAAGATCTGTGATTTTGGCCTGGCC AGAGACATCATGCACGATTCCAACTACGTGTCAAAAGGCAGTACTTTCCTGCCTGTGAAGTGGATGGCAC CCGAGAGCATCTTCGACAACCTCTACACCACGCTGAGTGACGTCTGGTCCTATGGCATTCTGCTCTGGGA AATCTTTTCCCTTGGTGGCACACCCTATCCTGGCATGATGGTCGATTCTACTTTCTACAATAAGATCAAG AGTGGATACCGGATGGCCAAACCTGACCATGCCACCAGTGAAGTCTATGAGATCATGGTGCAGTGCTGGA ACAGTGAGCCCGAGAAGAGACCCTCCTTCTACCACCTCAGCGAGATAGTGGAGAACCTGTTGCCGGGACA ATACAAGAAGAGTTATGAAAAGATTCACCTGGACTTCCTAAAGAGTGACCATCCGGCCGTGGCACGCATG CGGGTGGACTCTGATAATGCGTACATCGGTGTCACTTACAAAAATGAAGAGGATAAGCTGAAGGACTGGG AAGGTGGCCTGGACGAACAGAGACTGAGCGCTGACAGTGGCTACATCATCCCCCTGCCAGACATTGACCC TGTTCCAGAGGAGGAAGACCTGGGCAAGAGGAACAGACACAGCTCACAGACTTCGGAAGAGAGTGCCATC GAGACAGGTTCCAGTAGTTCCACCTTCATCAAGAGAGAGGACGAGACCATCGAGGACATCGACATGATGG ATGACATTGGTATAGATTCCTCGGACCTGGTGGAGGACAGCTTCCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011058
Insert Size	3270 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011058.2, NP_035188.2
RefSeq Size	6553 bp
RefSeq ORF	3270 bp
Locus ID	18595
UniProt ID	P26618
Gene Summary	This gene encodes a member of the receptor tyrosine kinase family of proteins. Binding of platelet-derived growth factor protein ligands to this receptor triggers receptor dimerization and autophosphorylation, resulting in the activation of several downstream signaling pathways. Signaling through the encoded receptor plays a role in gastrulation and the development of nearly all organ systems. Mice lacking a functional copy of this gene reportedly exhibit defects in lung, skeleton, testis and the central nervous system, and die soon after birth. Alternative splicing and intronic polyadenylation of gene transcripts have been implicated in muscle regeneration and fibrosis in adult mice. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).

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