Slc4a10 (NM_001242381) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA4136; NCBE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229353 representing NM_001242381 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATGAAGAAGCCGTTG TGGATAGAGGTGGAACACGCTCTATTCTCAAAACACATTTTGAGAAAGAAGATTTAGAAGGTCATCGGAC ATTATTTATTGGAGTTCATGTGCCCCTGGGTGGAAGAAAAAGCCATCGTCGTCACAGGCATCGTGGTCAT AAGCACAGAAAGAGGGACAGAGAGAGAGATTCGGGACTGGAGGATGGAAGAGAGTCCCCTTCTTTTGACA CCCCATCGCAGAGGGTGCAGTTTATTCTTGGAACTGAGGACGATGATGAGGAGCACCTCCCTCATGACCT TTTCACAGAGCTGGATGAGATTTGCTGGCGTGAAGGGGAAGATGCTGAGTGGCGAGAGACAGCCAGGTGG TTGAAATTTGAAGAGGATGTGGAAGATGGAGGAGAAAGATGGAGTAAGCCCTATGTGGCCACGCTTTCAT TACACAGCTTGTTTGAGTTGAGAAGCTGCATCCTGAATGGAACTGTGCTACTGGACATGCATGCCAACAC GATAGAAGAAATTGCAGATATGGTCCTTGACCAGCAGGTCAGCTCAGGCCAGCTGAATGAAGATGTTCGC CACAGGGTCCACGAAGCATTGATGAAGCAGCATCATCACCAGAATCAGAAAAAACTGGCTAACAGGATTC CTATTGTCCGATCTTTTGCTGATATTGGCAAGAAACAATCAGAACCAAATTCCATGGATAAAAATGGTCA GGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGCTGAGAATAAAAATGATGTCAGCAGGGAAAACAGCACT GTAGACTTCAGCAAGGTTGATCTGCATTTTATGAAAAAGATTCCTCCGGGTGCTGAAGCTTCAAACATCT TGGTAGGAGAACTGGAGTTCCTAGACAGAACTGTGGTTGCCTTTGTCAGGTTGTCTCCAGCTGTCTTGCT CCAAGGACTTGCTGAAGTTCCAATCCCAAGCAGATTTCTGTTCATCCTTCTGGGACCCCTGGGAAAGGGT CAACAGTACCACGAGATTGGCAGATCGATTGCGACCTTAATGACTGATGAGGTGTTTCATGATGTTGCTT ACAAAGCTAAAGACCGCAATGACTTGGTATCAGGAATTGATGAGTTTCTGGATCAGGTTACCGTTCTTCC TCCTGGAGAATGGGATCCAAGCATACGAATAGAACCTCCCAAAAATGTCCCTTCCCAGGAGAAGAGGAAG ATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGCGAAGCTGAGCCACATGGAGGACACAGCGGACCTG AACTCCAGCGAACTGGGAGGATTTTTGGGGGACTTATATTAGATATCAAAAGAAAGGCTCCATTCTTCTG GAGTGACTTCAGGGATGCTTTCAGCCTGCAGTGCTTAGCATCGTTCCTGTTTCTCTACTGTGCATGCATG TCTCCTGTCATCACATTTGGAGGACTGTTGGGAGAAGCAACTGAAGGTCGTATAAGTGCAATCGAATCAC TCTTTGGAGCATCTATGACCGGGATAGCCTATTCTCTTTTTGGTGGACAGCCCCTGACCATATTAGGCAG CACAGGACCTGTTTTGGTGTTTGAAAAGATCTTGTTTAAGTTTTGCAAGGAATACGGCCTGTCGTACTTG TCCTTACGGGCCAGCATTGGGCTCTGGACTGCAACACTGTGCATCATCCTTGTGGCCACGGACGCGAGCT CACTCGTCTGCTACATCACCCGGTTTACCGAAGAGGCTTTTGCTTCTCTCATTTGCATCATTTTTATCTA TGAAGCCCTGGAGAAGTTGTTTGAGCTCAGTGAAACCTATCCAATCAATATGCACAATGATTTGGAACTG CTGACACAATACTCATGTAACTGTATGGAGCCACATAGTCCCAGCAATGACACACTGAAGGAATGGCGGG AGTCCAACCTTTCTGCCTCTGACATAATCTGGGGGAACCTAACTGTGTCAGAGTGCAGATCACTGCACGG GGAGTATGTCGGGCGAGCCTGTGGCCATGGCCACCCCTACGTGCCAGATGTTCTCTTCTGGTCGGTGATC CTGTTCTTCTCCACAGTTACCATGTCAGCCACCCTGAAGCAGTTCAAGACCAGCCGCTATTTCCCAACCA AGGTTCGATCCATAGTGAGTGATTTTGCGGTTTTTCTTACAATTCTGTGTATGGTTTTAATTGACTATGC CATTGGGATCCCATCACCAAAACTACAAGTACCAAGCGTTTTCAAGCCGACCAGAGACGACCGTGGCTGG TTTGTTACACCTTTGGGTCCAAACCCATGGTGGACAATCATAGCTGCCATCATCCCAGCTTTACTCTGTA CTATTCTGATTTTCATGGACCAGCAGATTACAGCTGTCATCATCAACAGAAAAGAGCACAAGCTAAAGAA AGGTTGTGGCTATCACCTGGATCTGTTAATGGTGGCAGTCATGCTCGGGGTCTGCTCCATTATGGGCCTG CCATGGTTTGTGGCTGCCACAGTTCTCTCCATCACTCATGTCAACAGCCTCAAGCTCGAATCAGAGTGCT CTGCTCCAGGAGAACAACCCAAGTTTCTCGGCATTCGGGAGCAGAGGGTGACCGGGCTCATGATTTTTAT TCTTATGGGTTCATCCGTTTTCATGACCAGCATTCTGAAGTTTATCCCCATGCCAGTGTTATACGGAGTG TTTCTTTATATGGGTGCTTCGTCTCTCAAAGGAATTCAGTTATTTGATAGAATAAAGCTCTTCTGGATGC CAGCCAAACATCAACCAGATTTCATCTATCTAAGGCACGTGCCCCTCCGGAAAGTCCATCTCTTCACAGT CATTCAGATGAGTTGTCTCGGCCTTCTGTGGATAATCAAAGTTTCGAGAGCTGCTATTGTCTTTCCTATG ATGGTGTTGGCACTAGTGTTTGTGAGAAAGTTGATGGACTTCTTGTTTACCAAACGGGAACTCAGCTGGC TTGATGATTTAATGCCTGAGAGTAAAAAGAAGAAACTTGAAGATGCTGAGAAAGAAGAAGAACAAAGTAT GCTAGCCATGGAGGACGAGGGCACAGTACAACTCCCACTGGAGGGACACTACAGAGACGACCCGTCTGTG ATCAATATTTCTGATGAAATGTCAAAGACTGCCATGTGGGGGAACCTTCTAGTTACTGCTGACAACTCAA AAGAAAAGGAGTCACGCTTTCCTTCTAAAAGCTCCCCTTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242381
Insert Size	3264 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242381.1, NP_001229310.1
RefSeq Size	5460 bp
RefSeq ORF	3264 bp
Locus ID	94229
UniProt ID	Q5DTL9
Gene Summary	Sodium/bicarbonate cotransporter which plays an important role in regulating intracellular pH (PubMed:10993873, PubMed:20566632). Has been shown to act as a sodium/bicarbonate cotransporter in exchange for intracellular chloride (PubMed:10993873, PubMed:20566632). Has also been shown to act as a sodium/biocarbonate cotransporter which is not responsible for net efflux of chloride, with the observed chloride efflux being due to chloride self-exchange (By similarity). Controls neuronal pH and may contribute to the secretion of cerebrospinal fluid (PubMed:18165320). Reduces the excitability of CA1 pyramidal neurons and modulates short-term synaptic plasticity (PubMed:26136660). Required in retinal cells to maintain normal pH which is necessary for normal vision (PubMed:23056253). In the kidney, likely to mediate bicarbonate reclamation in the apical membrane of the proximal tubules (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate in-frame splice junction at the 5' end of an exon, lacks an alternate in-frame exon, and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (5) lacks two alternate internal segments and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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