Cp (NM_001276248) Mouse Untagged Clone

CAT#: MC229349

Cp (untagged) - Mouse ceruloplasmin (Cp), transcript variant 3



  "NM_001276248" in other vectors (1)

CNY 10,450.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms D3Ertd555; D3Ertd555e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229349 representing NM_001276248
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTTTTGCTGCTTAGCACATTTATATTTTTGTATAGTTCCTTAGCCTTGGCAAGAGATAAGCATT
ATTTCATTGGAATTACTGAAGCAGTCTGGGACTATGCTTCTGGCACTGAAGAAAAGAAACTTATTTCAGT
TGACACGGAACAGTCCAATTTCTATCTTCAAAATGGTCCAGATCGTATTGGAAGAAAATATAAGAAGGCC
CTTTATTTTGAGTACACAGATGGCACCTTTAGTAAGACTATAGACAAACCAGCCTGGCTAGGGTTTTTAG
GCCCTGTCATCAAAGCTGAAGTTGAAGATAAAGTTTATGTTCACTTAAAGAACCTTGCCTCTAGGATCTA
CACTTTTCATGCACATGGGGTAACGTACACCAAGGAGTATGAGGGAGCCGTCTACCCTGACAACACCACT
GATTTTCAACGGGCTGATGACAAAGTGCTTCCCGGACAACAGTATGTGTATGTGCTGCATGCCAATGAGC
CAAGTCCTGGAGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCCCATGTTGATGCTCCAAAAGA
TATTGCATCAGGACTCATAGGACCTCTAATACTCTGTAAAAAAGGTTCTCTATATAAGGAAAAAGAGAAA
AATATTGACCAAGAATTTGTACTAATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTGGAAGATA
ACATCAAAACCTTCTGCTCTGAACCCGAGAAAGTTGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG
GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGCAGACAGAGTG
AAGTGGTACCTTTTTGGTATGGGTAATGAAGTTGATGTGCATTCAGCTTTCTTTCATGGCCAAGCCCTGA
CCAGCAGGAACTATCAAACCGATATAATCAACCTGTTCCCTGCCACCCTAATTGATGCTTATATGGTGGC
CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTAAACCATCTGAAAGCTGGGTTGCAGGCCTTT
TTCCAGGTTCGTGACTGTAACAAGCCCTCGCCAGAGGATAATATCCAAGATAGGCATGTGAGACACTATT
ACATTGCTGCTGAGGAGGTCATCTGGAATTATGCTCCTTCTGGGACGGACATCTTCACTGGAGAGAATTT
AACAGCTCTGGAAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCCACAAGAATTGGTGGCTCTTATAAA
AAAATGGCATATCGTGAGTACACAGATGGTTCCTTCACAAACCGAAAACAAAGAGGCCCTGATGAGGAAC
ATCTTGGAATCCTAGGTCCTGTCATTTGGGCAGAAGTAGGAGACACCATTAAAGTCACCTTTCATAACAA
AGGACAGCATCCTCTCAGCATTCAGCCAATGGGAGTAAGTTTCACTGCAGAAAATGAGGGAACATACTAT
GGCCCACCAGGTCGCTCCTCACAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACATACGAATGGA
CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTACTACTCTGGCGT
TGACCCCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT
GCTGATGGGAGACAGAAAGATGTAGACAAAGAGTTCTACTTGTTTCCCACAGTGTTTGATGAGAATGAGA
GTTTACTCTTAGATGATAATATCAGGATGTTCACAACTGCACCTGATCAAGTGGATAAGGAAGATGAAGA
CTTTCAGGAGTCTAATAAGATGCACTCCATGAATGGGTTCATGTATGGCAATCAGCCTGGCCTCAATATG
TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCTGATGTGCATGGGATATACT
TTTCAGGAAATACTTATCTGTCTAAAGGAGAAAGAAGAGACACTGCAAACCTATTCCCTCATAAAAGTCT
CACCCTTCTCATGAACCCTGACACAAAAGGGACTTTTGATGTTGAGTGCCTTACAACGGATCACTACACA
GGTGGCATGAAGCAAAAATACACTGTGAACCAGTGCCAGCGGCAGTTTGAAGATTTCACTGTCTACCTTG
GAGAAAGGACCTACTATGTGGCAGCCGTAGAGGTGGAATGGGATTACTCACCAAGCAGGGCCTGGGAAAA
GGAGCTGCATCATTTGCAAGAGCAAAATGTTTCAAATGTATTTTTGGATAAAGAAGAGTTTTTCATAGGC
TCAAAGTACAAGAAGGTTGTGTATCGCCAGTTTACTGACAGCTCATTCAGAGAACAGGTGAAGAGACGAG
CCGAAGACGAGCACTTGGGCATCCTTGGGCCACCAATTCATGCAAATGTTGGAGACAAAGTTAAAGTTGT
CTTTAAAAATATGGCAACCAGGCCATATTCAATACATGCCCATGGGGTGAAAACAGAGAGTTCTACAGTT
GTTCCAACGTTACCAGGTGAAGTTCGAACTTATACATGGCAAATTCCAGAAAGATCAGGGGCTGGAAGAG
AGGATTCAGCTTGTATCCCATGGGCTTATTACTCAACTGTGGATCGAGTTAAGGATCTCTATAGTGGGCT
AATAGGCCCATTGATTGTTTGTCGGAAGTCTTATGTGAAAGTATTCAGTCCTAAAAAGAAAATGGAGTTT
TTCCTTCTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAAAACATACTCTG
AACACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCCTAGAAAGCAATAAAATGCATGCTATTAATGG
GAAAATGTTTGGAAACCTACAAGGCCTCACAATGCACGTGAAAGATGAAGTCAACTGGTATGTGATGGGA
ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAG
TATACAGTTCTGATGTCTTTGACCTTTTCCCTGGAACATACCAAACCCTAGAAATGTTTCCCCAAACACC
TGGAACCTGGTTACTCCACTGCCACGTGACTGACCATGTCCATGCTGGGATGGCAACTACCTACACTGTT
TTACCAGTAGAACAAGTATCATCTCAGAGTTACAGGATGACCTGGAACATCCTCTATACACTACTAATCA
GCATCATTATTTTATTCCAAATGTCTACCAAGGAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001276248
Insert Size 3258 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001276248.1, NP_001263177.1
RefSeq Size 4561 bp
RefSeq ORF 3258 bp
Locus ID 12870
UniProt ID Q61147
Gene Summary The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
Transcript Variant: This variant (3) uses an alternate, in-frame acceptor splice site as well as an alternate 3' exon structure, compared to variant 2. It encodes isoform c which is longer and has a distinct C-terminus, compared to isoform b. Both variants 3 and 4 encode the same isoform (c).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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