Sema6d (NM_001291000) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110067B02Rik; AA409156; D330011G23; mKIAA1479
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229333 representing NM_001291000 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTTCCTTCTGCTTTGGTTCTGCGTGCTGTTCCTTCTGGTCTCCAGGTTACGGGCGGTCAGCTTCC CAGAAGACGATGAGCCCCTCAACACGGTTGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG CCCTTCAGGCAACGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT ATTGCTGGCAGGGATCAAGTCTATACAGTGAACTTAAATGAAATCCCCCAAACAGAGGTGATACCAAGCA AGAAGCTGACGTGGAGGTCCAGACAGCAGGATCGAGAAAATTGTGCTATGAAAGGCAAGCATAAAGATGA ATGCCACAACTTCATCAAAGTCTTTGTCCCAAGAAATGATGAGATGGTTTTTGTCTGTGGTACCAATGCT TTCAACCCGATGTGCAGATACTATAGGTTGAGAACGTTAGAGTATGATGGGGAAGAAATTAGTGGCCTGG CACGATGCCCGTTTGATGCCCGACAAACCAATGTCGCCCTCTTTGCTGATGGAAAACTCTATTCTGCCAC AGTGGCTGATTTCCTGGCCAGTGATGCTGTCATTTACAGAAGCATGGGAGATGGATCTGCCCTTCGCACA ATAAAATACGATTCCAAGTGGATCAAAGAACCACACTTCCTTCATGCCATAGAATATGGAAACTATGTCT ATTTCTTCTTCAGAGAAATCGCCGTGGAACATAATAACTTAGGCAAGGCTGTGTATTCCCGCGTGGCTCG CATTTGTAAAAACGACATGGGTGGCTCACAGCGGGTCCTGGAGAAACACTGGACTTCCTTCCTTAAGGCT CGGCTGAACTGCTCCGTTCCTGGAGATTCCTTTTTCTACTTCGACGTCCTGCAGTCTATAACAGACATAA TCCAAATCAATGGCATCCCCACTGTGGTTGGGGTCTTCACCACACAGCTCAACAGCATTCCTGGTTCTGC AGTCTGTGCCTTTAGCATGGACGACATTGAGAAAGTGTTCAAAGGGCGGTTCAAAGAGCAGAAAACCCCA GACTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTACCAAAACCAAGGCCTGGCTGTTGTGCCAAACACG GCCTCGCAGAAGCTTACAAGACCTCCATCGACTTTCCAGATGACACCCTGGCTTTCATCAAGTCCCACCC GCTGATGGACTCTGCCGTCCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACACGGGTCAGGTACAGG TTGACAGCCATCGAAGTGGACCGTTCAGCAGGGCCATACCAAAACTACACAGTCATCTTTGTTGGCTCTG AAGCTGGCGTGGTACTTAAAGTTTTGGCAAAGACCAGTCCTTTCTCTCTGAATGACAGTGTATTACTCGA AGAGATTGAAGCTTATAACCCAGCCAAGTGCAGCGCCGAGAGTGAGGAGGACAGAAAGGTGGTCTCATTA CAGCTGGACAAGGATCACCATGCTTTATACGTGGCCTTCTCTAGCTGCGTGGTCCGCATCCCCCTCAGCC GCTGTGAGCGCTACGGATCGTGTAAAAAGTCTTGCATTGCATCACGTGACCCGTACTGTGGTTGGTTAAG CCAGGGAGTTTGTGAGAGAGTGACCCTAGGGATGCTCCCTGGAGGATATGAGCAGGACACGGAGTACGGC AACACAGCCCACCTAGGGGACTGCCACGAAAGTTTGCCTCCTTCAACTACACCAGATTACAAAATATTTG GCGGTCCAACATCTGACATGGAGGTATCCTCATCTTCTGTTACCACTGTGGCAAGTAGCCCAGAAATTAC ATCTAAAGTGATTGATACCTGGAGACCTAAACTGACGAGCTCCCGGAAATTTGTAGTTCAAGATGACCCA AATACTTCTGATTTTACTGATACTATATCAGGTGTACGGTGGGAAGTCCAGTCTGGAGAATCCAATCAGA TGGTCCACATGAATGTCCTCATCACCTGCGTGTTTGCCGCTTTTGTCTTGGGCGCGTTCATCGCAGGAGT GGCCGTGTACTGCTACCGTGACATGTTCGTTCGGAAGAACAGAAAGATCCATAAAGACGCAGAATCCGCC CAGTCGTGCACAGACTCCAGCGGAAGCTTCGCCAAGCTGAACGGCCTCTTTGACAGCCCCGTCAAGGAAT ACCAGCAGAACATTGATTCTCCCAAACTCTACAGCAACCTGCTGACCAGTCGGAAGGAACTGCCACCAAA CACGGATACAAAGTCCATGGCCGTGGACCACAGAGGCCAGCCTCCCGAGCTGGCTGCTCTCCCCACGCCG GAATCCACACCTGTCCTCCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCTGAGAAGGCCCACAGCC ACGGTGCTTCAAGGAAAGAACACCCCCAGTTTTTTCCTTCTAGTCCTCCACCCCATTCCCCATTGAGTCA CGGGCATATCCCCAGTGCCATCGTTCTTCCAAACGCCACTCACGACTACAATACATCCTTCTCCAACTCG AATGCCCACAAAGCCGAAAAGAAGCTTCAGAGCATGGATCACCCTCTTACGAAGTCATCCAGTAAGCGGG AGCACCGGCGGTCTGTGGATTCCAGGAATACTCTCAATGATCTCCTGAAGCATCTAAATGACCCAAACAG TAACCCCAAAGCCATCCTGGGAGAGATCCATATGGCTCATCAAACCCTCATGCTGGACCCGGTGGGACCA ATGGCTGAGGTCCCACCCAAGGTCCCTAACCGGGAGGCATCTCTATACTCCCCTCCCTCCACACTCCCCA GAAATAGTCCAACCAAGAGAGTAGATGTCCCCACCACTCCTGGGGTGCCAATGACTTCTCTGGAAAGACA AAGGGGTTATCACAAAAATTCCTCCCAGAGGCACTCTATATCTGCCGTGCCTAAAAACTTAAACTCACCA AATGGTGTTTTGTTATCTAGACAGCCGAGTATGAACCGTGGAGGCTATATGCCCACCCCAACAGGGGCGA AGGTGGACTATATTCAGGGGACACCGGTGAGTGTTCATCTGCAGCCCTCCCTCTCCAGACAGAGCAGCTA TACCAGTAATGGCACCCTCCCCAGGACGGGACTAAAGAGGACACCATCCTTAAAACCTGATGTGCCACCA AAGCCTTCCTTTGTTCCGCAAACCACATCTGTCAGACCACTGAACAAGTACACGTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291000
Insert Size	3210 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291000.1, NP_001277929.1
RefSeq Size	6509 bp
RefSeq ORF	3210 bp
Locus ID	214968
UniProt ID	Q76KF0
Gene Summary	Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is longer than isoform Sema6D-1.

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