Herc4 (NM_030114) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700056O17Rik; 4921531D01Rik; 9530080M15Rik; mKIAA1593
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229321 representing NM_030114 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTGCTGGGGCAATGCATCCTATGGACAACTAGGTTTGGGAGGAATTGATGAAGAAATTGTACTAG AGCCCAGGAGAAGTGACTTTTTCGTGAACAAAAAGGTCCGAGATGTAGGCTGTGGACTCAGGCACACTGT GTTTGTCCTGGATGATGGGACTGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAG TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAGAATATCGTAGCTGTTGCGTGTGGAGAAG CTCACACGTTAGCGCTGAATGACAAGGGCCAGGTGTATGCTTGGGGTCTCGACTCTGACGGACAGCTTGG CCTACAGGGATCAGAGGAATGTATCAGAGTACCCAGAAATATTAAAAGTCTTTCGGATATCCAGATAGTA CAGGTTGCGTGTGGTTACTATCATTCGCTTGCACTTTCTAAAGCAAGTGAAGTTTTCTGTTGGGGACAGA ATAAATATGGCCAGCTGGGTCTAGGCATTGATTGTCAAAAGCAAACTTCACCACAGCTGATTAAGTCTTT GCTTGGAATACCATTCATGCAAGTCGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCCGGAGCT ATCTTTGGATGGGGACGTAACAAATTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGCGGAGAAGATCATACTGCTGCATT AACCAAGGAAGGTGGAGTGTTTACCTTTGGAGCTGGGGGCTATGGCCAGCTGGGTCATAATTCTACCAGT CATGAGATAAATCCCAGGAAAGTTTTTGAACTCATGGGAAGCATTGTTACTCAGGTCGCTTGTGGAAGGC AGCATACCTCTGCATTTGTTCCTTCTTCAGGACGAATTTACTCCTTTGGTCTTGGTGGTAATGGGCAGTT GGGAACTGGTTCAACCAGCAACAGAAAAAGCCCTTTCACTGTAAAAGGAAACTGGTTTTCTTATAATGGA CAGTGTCCACAAGATATTGGTTCTGAAGACTATTTCTGTGTCAAAAGAATTTTCTCAGGTGGAGATCAAA GCTTTTCACATTACTCTAGTCCTCAGAACTGTGGACCTCCAGATGACTTTAGATGCTCCGACCCTTCCAA GCAGATCTGGACAGTGAACGAAGCTCTGATTCAGAAATGGCTCAGCTACCCCTCTGGAAGGTTCCCTGTG GAGATAGCCAATGAGATAGATGGAACATTTTCTTCATCTGGCTGCCTAAATGGAAGTTTTCTGGCTATTA GCAATGACGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAACGCTGCTAGACTCTTATT CCACAAACTTATACAACCTGATCATCCTCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTA ATCCCTAAACTGACTAGTTCCCTACCTGATGTTGAAGCTCTGAGGTTTTATCTTACCCTGCCAGAGTGTC CCCTGATGAGTGACTGCAACAATTTCACAACAATAGCAATCCCCTTTGGTACAGCTCTTGTGAACCTAGA AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGCATTCCCCCTTCTGAAAGAA GAATTTTCAACAGTTTTCTTCATACCGCATTAAAGGTTTTAGAAATATTGCATAGGGTAAATGAGAAAAC AGGACAACTTATTCAATATGACAAGTTTTACATCCATGAAGTCCAAGAGCTGATAGACATAAGGAATGAC TATATCAACTGGGTCCAGCAGCAAGCCTATGGAGTGGATGTCAGCCATGGAGTAACTGAGTTGGCAGATA TCCCGGTGACAATTTGTACATATCCATTTGTATTTGATGCCCAGGCAAAAACTACTCTGCTCCAGACAGA TGCAGTCTTGCAGATGCAGATGGCCATTGACCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCA GTGATCGAGTCTGTGAATCCTTGCTTAATTCTAGTTGTTCGCAGAGAGAATATTGTAGGAGATGCGATGG AAGTCCTCAGGAAAACCAAGAATATAGATTATAAAAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGC TGTTGATGCAGGAGGTGTTCGCAAAGAATTTTTCTTGCTTATCATGAGGGAATTGTTGGATCCTAAATAC GGCATGTTTCGATATTATGAAGATTCCAGGCTCATTTGGTTTTCAGATAAGACATTTGAAGACAGTGATT TGTTCCACTTGATCGGTGTTATCTGTGGATTAGCAATTTATAATTTTACCATTGTGGACCTCCATTTCCC TTTGGCTTTATATAAGAAACTGCTGAAAAGGAAGCCGTCCCTGGATGATCTGAAAGAGCTGATGCCAGCC GTCGGGAGAAGCATGCAGCAATTGCTGGACTATCCAGAAGATGACATAGAGGAAACATTTTGTCTAAACT TTACGATCACAGTTGAGAACTTCGGTGCAACAGAAGTAAAAGAGCTGGTTCTGAACGGGGCAGACACTGC TGTTAATAGACAGAATCGGCAGGAGTTTGTTGATGCCTATGTGGATTACATATTCAATAAATCAGTGGCA TCTTTATTTGATGCTTTCCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTTCTTCTGCTCTTCCAGC CTAATGAATTACAAGCTATGGTTATTGGGAATACAAATTATGATTGGAAAGAACTGGAGAAGAATACAGA ATACAAAGGAGAGTATTGGGCAGACCATCCCACGATAAAAATTTTTTGGGAAGTGTTTCATGAATTACCA CTAGAGAAGAAGAAACAATTTCTATTATTTTTGACAGGTAGTGATCGGATTCCCATTCTTGGTATGAAGA GTCTGAAACTAGTCATCCAGTCAACAGGAGGTGGTGAGAGCTATCTCCCGGTTTCCCATACCTGTTTTAA TCTTCTAGATCTCCCAAAATATACAGAGAAAGAAACCCTCCGATGTAAACTGATCCAAGCTATTGATCAC AATGAAGGCTTCAGTTTAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030114
Insert Size	3174 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030114.2, NP_084390.1
RefSeq Size	4043 bp
RefSeq ORF	3174 bp
Locus ID	67345
UniProt ID	Q6PAV2
Gene Summary	Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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