Tlr7 (NM_001290756) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229313 representing NM_001290756 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTTCGATGTGGACACGGAAGAGACAAATTTTGATCTTTTTAAATATGCTCTTAGTTTCTAGAG TCTTTGGGTTTCGATGGTTTCCTAAAACTCTACCTTGTGAAGTTAAAGTAAATATCCCAGAGGCCCATGT GATCGTGGACTGCACAGACAAGCATTTGACAGAAATCCCTGAGGGCATTCCCACTAACACCACCAATCTT ACCCTTACCATCAACCACATACCAAGCATCTCTCCAGATTCCTTCCGTAGGCTGAACCATCTGGAAGAAA TCGATTTAAGATGCAATTGTGTACCTGTTCTACTGGGGTCCAAAGCCAATGTGTGTACCAAGAGGCTGCA GATTAGACCTGGAAGCTTTAGTGGACTCTCTGACTTAAAAGCCCTTTACCTGGATGGAAACCAACTTCTG GAGATACCACAGGATCTGCCATCCAGCTTACATCTTCTGAGCCTTGAGGCTAACAACATCTTCTCCATCA CGAAGGAGAATCTAACAGAACTGGTCAACATTGAAACACTCTACCTGGGTCAAAACTGTTATTATCGAAA TCCTTGCAATGTTTCCTATTCTATTGAAAAAGATGCTTTCCTAGTTATGAGAAATTTGAAGGTTCTCTCA CTAAAAGATAACAATGTCACAGCTGTCCCCACCACTTTGCCACCTAATTTACTAGAGCTCTATCTTTATA ACAATATCATTAAGAAAATCCAAGAAAATGATTTTAATAACCTCAATGAGTTGCAAGTTCTTGACCTAAG TGGAAATTGCCCTCGATGTTATAATGTCCCATATCCGTGTACACCGTGTGAAAATAATTCCCCCTTACAG ATCCATGACAATGCTTTCAATTCATTGACAGAATTAAAAGTTTTACGTTTACACAGTAATTCTCTTCAGC ATGTGCCCCCAACATGGTTTAAAAACATGAGAAACCTCCAGGAACTAGACCTCTCCCAAAACTACTTGGC CAGAGAAATTGAGGAGGCCAAATTTTTGCATTTTCTTCCCAACCTTGTTGAGTTGGATTTTTCTTTCAAT TATGAGCTGCAGGTCTACCATGCATCTATAACTTTACCACATTCACTCTCTTCATTGGAAAACTTGAAAA TTCTGCGTGTCAAGGGGTATGTCTTTAAAGAGCTGAAAAACTCCAGTCTTTCTGTATTGCACAAGCTTCC CAGGCTGGAAGTTCTTGACCTTGGCACTAACTTCATAAAAATTGCTGACCTCAACATATTCAAACATTTT GAAAACCTCAAACTCATAGACCTTTCAGTGAATAAGATATCTCCTTCAGAAGAGTCAAGAGAAGTTGGCT TTTGTCCTAATGCTCAAACTTCTGTAGACCGTCATGGGCCCCAGGTCCTTGAGGCCTTACACTATTTCCG ATACGATGAATATGCACGGAGCTGCAGGTTCAAAAACAAAGAGCCACCTTCTTTCTTGCCTTTGAATGCA GACTGCCACATATATGGGCAGACCTTAGACTTAAGTAGAAATAACATATTTTTTATTAAACCTTCTGATT TTCAGCATCTTTCATTCCTCAAATGCCTCAACTTATCAGGAAACACCATTGGCCAAACTCTTAATGGCAG TGAACTCTGGCCGTTGAGAGAGTTGCGGTACTTAGACTTCTCCAACAACCGGCTTGATTTACTCTACTCA ACAGCCTTTGAAGAGCTCCAGAGTCTTGAAGTTCTGGATCTAAGTAGTAACAGCCACTATTTTCAAGCAG AAGGAATTACTCACATGCTAAACTTTACCAAGAAATTACGGCTTCTGGACAAACTCATGATGAATGATAA TGACATCTCTACTTCGGCCAGCAGGACCATGGAAAGTGACTCTCTTCGAATTCTGGAGTTCAGAGGCAAC CATTTAGATGTTCTATGGAGAGCCGGTGATAACAGATACTTGGACTTCTTCAAGAATTTGTTCAATTTAG AGGTATTAGATATCTCCAGAAATTCCCTGAATTCCTTGCCTCCTGAGGTTTTTGAGGGTATGCCGCCAAA TCTAAAGAATCTCTCCTTGGCCAAAAATGGGCTCAAATCTTTCTTTTGGGACAGACTCCAGTTACTGAAG CATTTGGAAATTTTGGACCTCAGCCATAACCAGCTGACAAAAGTACCTGAGAGATTGGCCAACTGTTCCA AAAGTCTCACAACACTGATTCTTAAGCATAATCAAATCAGGCAATTGACAAAATATTTTCTAGAAGATGC TTTGCAATTGCGCTATCTAGACATCAGTTCAAATAAAATCCAGGTCATTCAGAAGACTAGCTTCCCAGAA AATGTCCTCAACAATCTGGAGATGTTGGTTTTACATCACAATCGCTTTCTTTGCAACTGTGATGCTGTGT GGTTTGTCTGGTGGGTTAACCATACAGATGTTACTATTCCATACCTGGCCACTGATGTGACTTGTGTAGG TCCAGGAGCACACAAAGGTCAAAGTGTCATATCCCTTGATCTGTATACGTGTGAGTTAGATCTCACAAAC CTGATTCTGTTCTCAGTTTCCATATCATCAGTCCTCTTTCTTATGGTAGTTATGACAACAAGTCACCTCT TTTTCTGGGATATGTGGTACATTTATTATTTTTGGAAAGCAAAGATAAAGGGGTATCAGCATCTGCAATC CATGGAGTCTTGTTATGATGCTTTTATTGTGTATGACACTAAAAACTCAGCTGTGACAGAATGGGTTTTG CAGGAGCTGGTGGCAAAATTGGAAGATCCAAGAGAAAAACACTTCAATTTGTGTCTAGAAGAAAGAGACT GGCTACCAGGACAGCCAGTTCTAGAAAACCTTTCCCAGAGCATACAGCTCAGCAAAAAGACAGTGTTTGT GATGACACAGAAATATGCTAAGACTGAGAGTTTTAAGATGGCATTTTATTTGTCTCATCAGAGGCTCCTG GATGAAAAAGTGGATGTGATTATCTTGATATTCTTGGAAAAGCCTCTTCAGAAGTCTAAGTTTCTTCAGC TCAGGAAGAGACTCTGCAGGAGCTCTGTCCTTGAGTGGCCTGCAAATCCACAGGCTCACCCATACTTCTG GCAGTGCCTGAAAAATGCCCTGACCACAGACAATCATGTGGCTTATAGTCAAATGTTCAAGGAAACAGTC TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290756
Insert Size	3153 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290756.1, NP_001277685.1
RefSeq Size	3862 bp
RefSeq ORF	3153 bp
Locus ID	170743
UniProt ID	P58681
Gene Summary	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the 5' region, compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a).

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