Ogt (NM_001290535) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110038P24Rik; 4831420N21Rik; AI115525; Ogtl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229301 representing NM_001290535 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGTACAGGGTTAGCTGAGTTGGCACATCGAGAATATCAGG CAGGAGATTTTGAGGCAGCTGAGAGACACTGCATGCAGCTCTGGAGACAAGAGCCTGACAATACTGGTGT TCTTTTATTACTTTCATCTATACACTTCCAGTGTCGAAGGCTGGACAGATCTGCTCATTTTAGCACCTTG GCAATTAAACAGAATCCCCTTCTAGCAGAAGCCTATTCGAATTTGGGAAATGTGTACAAGGAAAGAGGGC AGTTGCAGGAAGCAATCGAGCATTATCGACATGCCTTGCGGCTGAAGCCTGATTTCATTGATGGTTATAT TAACCTGGCAGCAGCCTTGGTAGCAGCAGGTGACATGGAAGGAGCAGTACAAGCCTATGTCTCTGCTCTT CAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGGGGAACCTGCTCAAAGCCCTGGGTCGCTTGG AAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCAACCAAACTTTGCAGTAGCCTGGAGTAATCT CGGCTGTGTTTTCAATGCACAAGGGGAGATTTGGCTGGCTATTCATCACTTTGAAAAGGCTGTCACCCTT GACCCAAATTTTCTGGATGCTTATATCAATTTAGGAAATGTCTTGAAAGAGGCACGCATTTTTGACAGAG CTGTCGCAGCTTATCTTCGTGCCTTAAGTTTGAGCCCAAATCATGCGGTGGTGCACGGCAACCTGGCTTG TGTGTACTACGAGCAAGGCCTAATAGACCTGGCCATTGATACCTACAGGAGAGCTATCGAACTGCAACCC CATTTCCCCGATGCTTACTGCAACCTAGCAAATGCTCTCAAAGAGAAGGGCAGTGTTGCTGAAGCAGAAG ATTGTTATAACACAGCTCTTCGTCTGTGTCCTACTCATGCAGACTCTTTGAATAACCTTGCCAACATCAA ACGGGAACAGGGCAACATTGAAGAGGCAGTTCGCCTGTATCGCAAAGCATTAGAAGTCTTCCCAGAGTTT GCTGCTGCACATTCCAATTTAGCAAGTGTACTGCAACAGCAGGGCAAGCTGCAGGAAGCACTGATGCACT ATAAAGAAGCCATACGAATTAGTCCTACATTTGCTGATGCTTATTCCAATATGGGAAACACTCTAAAGGA GATGCAGGATGTGCAGGGCGCTTTGCAGTGTTATACTCGTGCCATCCAGATTAATCCTGCCTTTGCTGAT GCACACAGCAATCTGGCCTCCATTCACAAGGATTCAGGGAATATCCCAGAAGCAATAGCTTCTTACCGCA CAGCTCTGAAACTTAAGCCTGACTTTCCTGATGCTTATTGTAACTTGGCTCATTGCCTACAGATTGTCTG TGATTGGACAGACTATGATGAGCGGATGAAGAAATTGGTTAGTATTGTAGCTGAGCAGCTAGAGAAGAAT AGACTGCCTTCTGTCCATCCTCACCATAGCATGCTGTACCCTCTTTCCCATGGCTTCAGGAAGGCTATTG CAGAGAGGCATGGGAATCTCTGCTTGGATAAGATTAATGTCCTTCATAAACCACCATATGAACATCCAAA AGACTTGAAGCTCAGTGATGGCCGATTGCGTGTAGGCTATGTGAGTTCTGACTTCGGGAATCACCCTACT TCACACCTTATGCAGTCTATTCCAGGCATGCATAATCCTGATAAGTTTGAGGTATTCTGCTATGCCTTGA GCCCGGATGATGGTACAAACTTTCGAGTGAAGGTGATGGCGGAAGCCAATCATTTCATTGATCTTTCTCA GATTCCTTGTAATGGAAAAGCAGCCGACCGCATCCACCAAGATGGAATTCACATCCTTGTGAATATGAAT GGGTATACCAAGGGTGCTCGGAATGAGCTCTTTGCTCTTAGGCCAGCTCCTATTCAGGCCATGTGGCTGG GCTACCCTGGGACTAGTGGTGCACTGTTCATGGATTACATCATCACTGATCAGGAAACTTCCCCAGCTGA AGTTGCAGAGCAGTATTCTGAGAAACTGGCTTATATGCCCCATACTTTCTTTATTGGTGATCATGCTAAT ATGTTCCCTCACCTGAAGAAAAAAGCAGTCATCGATTTTAAATCCAATGGGCACATTTATGATAATCGGA TAGTTCTGAATGGCATCGATCTCAAAGCATTTCTCGATAGCCTACCCGATGTGAAGATTGTCAAGATGAA ATGTCCTGATGGAGGTGACAATCCAGACAGCAGTAACACAGCTCTTAATATGCCCGTTATTCCCATGAAT ACGATTGCAGAAGCAGTAATTGAAATGATTAACAGAGGGCAGATTCAGATAACAATTAACGGATTCAGTA TTAGCAATGGACTGGCGACTACACAGATTAATAATAAGGCTGCAACCGGAGAGGAAGTTCCCCGTACCAT TATTGTAACCACCCGTTCCCAGTATGGGCTACCAGAAGATGCCATTGTGTACTGTAACTTTAATCAGTTA TATAAAATTGACCCATCTACCCTGCAGATGTGGGCAAATATTCTGAAACGTGTGCCTAACAGCGTGCTTT GGCTGTTGCGTTTTCCAGCAGTAGGAGAACCCAATATTCAACAATATGCACAAAATATGGGCCTTCCCCA GAACCGTATCATTTTCTCACCTGTGGCTCCTAAAGAGGAGCATGTCAGGAGAGGTCAGCTGGCTGATGTC TGCCTGGATACTCCTTTGTGTAATGGACACACCACAGGGATGGATGTTCTCTGGGCAGGAACACCCATGG TGACTATGCCAGGAGAGACTCTTGCCTCTCGAGTTGCAGCTTCTCAGCTTACTTGTCTAGGATGTCTCGA GCTCATTGCTAAAAGCAGACAGGAATATGAAGACATAGCTGTGAAACTGGGAACCGATCTAGAATACCTG AAGAAAATTCGTGGCAAAGTCTGGAAACAGAGAATATCTAGCCCTCTGTTCAACACCAAACAATACACAA TGGAATTAGAGCGACTTTATCTGCAGATGTGGGAGCATTATGCAGCTGGCAACAAACCTGACCACATGAT TAAGCCTGTTGAAGTCACCGAGTCAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290535
Insert Size	3111 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290535.1, NP_001277464.1
RefSeq Size	5218 bp
RefSeq ORF	3111 bp
Locus ID	108155
UniProt ID	Q8CGY8
Gene Summary	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed:29465778). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (By similarity).Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:23337503, PubMed:23395176). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (By similarity). Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1 (By similarity). Glycosylates HOXA1 (PubMed:29465778).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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