Mllt10 (NM_001252561) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Af10; B130021D15Rik; D630001B22Rik; mKIAA4140
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229254 representing NM_001252561 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGATGTGAGCTGTGTCCCCATAAAGATGGAGCTTTAAAAAGAACAGATAATGGGGGTTGGGCTC ATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTT ACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAA GCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGACAGGCTTTCCATGTAACATGTGCTC AGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAA ATACCACTTTAGTAAGCTGAAAAAGAGCAAACGGGGATCTAATAGGTCATATGAGCAAAGTTTAAGTGAC TCTTCCTCTCACTCTCAGGATAAGCATCATGAGAAAGAGAAAAAAAAATATAAAGAAAAGGACAAACACA AACAAAAACACAAGAAGCAACCAGAACCGTCACCTGCCTTGGTTCCTTCTTTGACTGTTACTACAGAAAA AACTTATACAAGCACTAGCAACAACTCCATATCTGGATCATTGAAGCGCTTGGAAGATACTGCAGCAAGA TTTACAAATGCAAATTTCCAGGAAGTCTCTGCCCATACCTCTAGTGGAAAAGATGTTTCAGAGGCTAGAG GGTCAGAGGGCAAAGGGAAGAAATCTTCAGCTCACAGCTCAGGTCAAAGGGGAAGGAAGCCTGGTGCAGG AAGAAATCCAGGAACAGCTGTGTCAGCATCTAGCCCCTTTCCACAAGGCAGTTTTTCAGGAACTCCAGGC AGTGTAAAGTCATCTTCTGGAAGTTCAGTGCAGTCTCCCCAGGACTTCTTAAGCTTTACAGACTCAGATC TGCGAAGTGACAGTTACACTCACACCCAGCAGCCATCATCAACCAAAGATGTACATAAAGGAGAGTCTGG AAGCCAGGAAGCGGCAGTAAACAGTTTTAGCTCTTTAGTTGGTCACCCTGTGACCTCGACTGTTATTTCA CAGCCTAAAAGCTTTGACAATTCACCTGGAGAGTTGGGTAGTTCGAGCCTCCCTACAGCAGGATATAAGC GGGCTCAGACTTCTGGTATAGAAGAAGAAGCTGTAAAGGAAAAGAAACGAAAAGGAAATAAGCAAAGCAA ACATGGCCCCGGCCGACCCAAAGGAAACAAAAATCAAGAAAATGTTTCTCACCTCTCAGTTTCTTCTGCT TCACCAACATCGTCCGTAGCATCAGCTGCAGGAAGTGTAACAAGCTCTAGTCTTCAGAAATCTCCTACGC TGCTCAGGAATGGAAGTTTGCAGAGTCTTAGTGTTGGCTCGTCTCCAGTTGGCTCAGAAATTTCCATGCA GTATCGACATGATGGAGCTTGTCCAACAACTACCTTCTCAGAGTTGCTGAATGCAATACACAATGGTATT TATAACAGCAATGATGTAGCCGTATCCTTTCCAAACGTGGTGTCTGGCTCAGGATCTAGTACTCCTGTCT CTAGCTCTCATATACCTCAGCAGTCATCTGGTCATTTGCAACAGGTGGGAGCTCTCTCCCCCTCAGCTGC GTCATCTGTAACCCCTGCTGCTGCTACAACTCAGGCAAATACCGTTTCTGGATCTTCTCTCAGTCAGGCA CCAGCCCATATGTATGGCAGTAGATTAAATCAGAATCCATCCATGGCAGTTCTCATAGCTCAGTCTGAAA GCAGCCAAACAGATCAAGATCTTGGAGACAATGCCCGTAGCCTGGGTGGCCGAGGAAGCTCGCCCCGAGG AAGCCTCTCTCCGAGATCCCCTGTGAGCAACTTACAGCTTCGCTATGATCAACCGAGCAATAGCAGTTTG GAAACTGTGCCTCCAGTAGCAGCAAGTATTGAACAGCTCTTGGAGAGGCAGTGGAGTGAAGGACAGCAGT TTTTACTAGAGCAGGGGACTCCTGGTGACATTCTAGGAATGTTAAAGTCATTACATCAACTTCAAGTTGA AAATCGAAGATTAGAAGAACAGATCAAAAATTTGACTGCCAAGAAAGAACGTCTTCAGTTGTTGAATGCA CAGCTTTCAGTGCCTTTCCCAGCCATAACGACAAACCCCAGTCCGTCCCATCAGATGCACACATACACAG CACAGACTGCTCCTCCTCCTGATTCCCTGAACAGCAGTAAGAGCCCACATATAGGAAACAGCTTTTTACC TGACAATTCTCTTCCTGTATTAAATCAGGACTTGACCTCCAGTGGACAAAGCACCAGCAGTTCTTCTGCT CTTTCTACTCCCCCTCCTGCTGGCCAGAGTCCTGCTCAGCAGAGCTCTGGAGTCAGTGGGGTTCAGCAGG TGAATGGCGTGACAGTGGGGGCACTAGCTAGTGGGATGCAGACCGTCACATCCACCATCCCAGCTGTGTC CGCAGTGGGTGGAATAATTGGAGCTCTGCCAGGTAACCAACTGGCAATTAACGGCATTGTGGGAGCTTTA AATGGTGTGATTCAGACTCCAGTCACAATCTCCCAGAACCCTGCCCCTCTCACCCACACCAGTGTACCAC CTAATGCAGCGCATCCAATGCCGGCCGCCGCACTGACCAACAGTGCCTCGGGACTAGGGCTGCTGTCGGA CCAGCAGAGACAGATGTTTATCCAGCAGCAGCAGTTCCAGCAGCTGTTAAACTCCCAGCAGCTCACACCC GAACAGCATCAGGCCTTCCTATACCAGCTGATGCAGCAGCAGCACCACCCGCCTGAGCTCCAGCAGCTGC AGCTCCCGGGACCCACGCAGATCCCCATCAACAATCTCCTTGCAGGTGCTCAGGCGCCTCCACTCCACAC AGCTACCACCAACCCCTTCCTCACCATCCACGGGGACAGCACGAGCCAGAAGGTCACAAGACTTAGTGAT AAAACTGGGCCTGTAGCTCAAGAGAAAAGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252561
Insert Size	2973 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252561.1, NP_001239490.1
RefSeq Size	5116 bp
RefSeq ORF	2973 bp
Locus ID	17354
Gene Summary	Probably involved in transcriptional regulation. Binds to cruciform DNA (By similarity). In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) includes an alternate exon and uses an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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