Sema6c (NM_001272024) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA1869; Semay
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229225 representing NM_001272024 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGTGCCCCCCACTCCATGCCCCTGCTGCTCCTGCTGCTGTTGCTGTCATCGCTCCCCCAAGCCC AGGCTGCCTTTCCCCAGGACCCCACCCCTCTGTTGACCTCTGACCTTCAAGGTGCCTCTCCATCATCCTG GTTCCGGGGCCTGGAGGACGATGCTGTGGCTGCGGAACTTGGGCTGGACTTTCAGAGATTCCTGACCTTG AACCGGACCTTGCTTGTGGCTGCCCGGGATCACGTTTTCTCCTTCGATCTTCAAGCCCAAGAAGAAGGGG AGGGGCTGGTGCCCAACAAGTTTCTGACATGGAGGAGTCAAGATATGGAGAACTGTGCAGTCCGGGGAAA GCTGACGGACGAATGCTACAACTACATCCGTGTTCTTGTTCCCTGGAACTCGCAGACACTTCTTGCCTGT GGAACAAATTCCTTCAGCCCTATGTGCCGCAGCTATGGGATAACATCTCTGCAACAGGAGGGTGAGGAGC TGAGTGGGCAGGCTCGATGCCCCTTTGATGCCACCCAGTCCACTGTGGCCATCTTTGCAGAGGGCAGTTT GTACTCAGCCACAGCAGCAGATTTCCAGGCCAGTGATGCTGTAGTTTACAGAAGTCTTGGACCTCAGCCT CCACTCCGTTCTGCAAAGTATGACTCCAAGTGGCTTCGAGAGCCACACTTTGTCTATGCGTTGGAGCATG GAGAGCACGTCTACTTCTTCTTCCGAGAAGTCTCTGTGGAGGATGCCCGCTTAGGGAGGGTGCAGTTTTC CAGGGTAGCCCGGGTATGTAAACGTGACATGGGTGGCTCACCTCGGGCCTTGGATCGCCACTGGACATCC TTCCTTAAGCTGAGGCTCAACTGCTCTGTCCCTGGGGACTCTACCTTCTACTTTGACGTCTTACAGTCCT TAACTGGGCCTGTGAACCTGCATGGCCGCTCCGCTCTCTTTGGGGTCTTCACTACTCAGACCAATAGCAT TCCTGGGTCTGCAGTCTGCGCCTTCTACCTAGATGATATTGAACGTGGCTTTGAGGGCAAGTTCAAGGAG CAGAGGAGTCTGGATGGGGCCTGGACTCCTGTGTCTGAGGACAAGGTCCCCTCACCCAGGCCAGGGTCCT GTGCAGGTGTGGGTGCAGCTGCCTCGTTCTCCTCCTCTCAAGACCTACCTGATGATGTCCTGCTCTTCAT CAAGGCACACCCGCTGCTGGATCCTGCTGTGCCACCCGCCACCCATCAACCGCTCCTCACTCTGACTAGC AGGGCCCTGCTGACCCAGGTAGCTGTGGACGGAATGGCTGGTCCCCACAGAAATACTACTGTCCTGTTTC TTGGCTCCAACGATGGGACAGTGCTGAAGGTGCTACCTCCAGGGGGTCAGTCTTTGGGCTCTGAGCCTAT CGTCTTGGAAGAGATTGATGCCTACAGCCATGCCCGGTGCAGTGGGAAGCGGTCACCCCGAGCTGCGCGG CGGATCATAGGGCTGGAGCTGGACACTGAGGGTCACAGGCTTTTTGTGGCCTTTCCTGGATGTATCGTCT ACCTCTCTCTCAGCCGGTGTGCCCGGCATGGAGCATGTCAGAGGAGCTGCCTGGCTTCTCTGGACCCATA CTGTGGATGGCATCGATCCAGAGGCTGCATGAGTATCAGGGGACCTGGTGGGACTGATGTCGATCTGACT GGGAACCAGGAATCCACAGAGCACGGTGACTGCCAAGATGGAGCGACCGGGAGTCAGTCTGGCCCTGGAG ATTCTGCTTATGTGCTTCTGGGTCCTGGCCCTTCCCCTGAGACCCCCAGTTCCCCCAGTGATGCCCAACA AGGGCCCCAGTCTTCCACTCTTGGAGCTCACACACAGGGCGTGCGCAGGGACCTTTCCCCAGCCTCAGCC TCCCGATCCATTCCCATCCCACTCCTCCTGGCCTGTGTGGCCGCGGCCTTCGCTTTGGGCGCCTCAGTCT CCGGTCTCTTGGTGTCCTGTGCTTGTCGTCGCGCGAACCGCCGTCGGAGCAAGGACATCGAGACCCCGGG GCTGCCGCGCCCCCTCTCCCTTCGCAGCCTGGCCCGGCTGCACGGTGGCGGTCCTGAGCCCCCGCCTCCG CCCAAGGATGGAGATGCAGCGCAAACGCCCCAGCTCTACACTACCTTCCTGCCTCCGCCCGACGGCGGAT CCCCACCGGAGCTGGCCTGCCTACCCACGCCGGAGACGACGCCCGAGCTGCCCGTGAAGCACCTCCGTGC CTCCGGGGGCCCCTGGGAATGGAACCAGAATGGGAACAACGCCTCGGAGGGCCCAGGCCGCCCACCACGG GGCTGCAGCGGGGCGGGCGGGCCCGCACCGCGAGTGCTGGTGAGGCCACCGCCCCCTGGCTGCCCCGGGC AGGCGGTAGAGGTGACCACGCTGGAGGAACTGCTGCGCTACCTGCACGGCCCTCAGCCGCCCAGGAAGGG TAGCGAACCTCTCGCCTCCGCCCCGTTCACCTCCCGGCCGCCCGCCTCGGAGCCCGGCGCCTCGCTGTTC GTGGACTCCAGCCCGATGCCGCGGGATGGCGTTCCGCCGCTCAGGCTCGACGTGCCACCCGAAGGCAAGC GCGCTGCCCCGAGCGGGCGGCCTGCTCTCTCGGCCCCAGCCCCGCGCCTGGGTGTCGGCGGCAGCCGCCG ATTGCCCTTTCCCACACACCGGGCGCCCCCGGGCCTGCTCACGCGAGTGCCCTCGGGAGGCCCGGCCAGG TACTCCGGGGGGCCCGGGAGGCACCTCCTGTACCTGGGCCGGCCCGAAGGCCATCGCGGCCGCTCCCTGA AGAGGGTGGACGTGAAGTCTCCGCTGTCGCCCAAGCCGCCCCTCGCCTCCCCGCCGCAGCCCGCCCCGCA CGGTGGTCATTTCAACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272024
Insert Size	2892 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272024.1, NP_001258953.1
RefSeq Size	4140 bp
RefSeq ORF	2892 bp
Locus ID	20360
UniProt ID	Q9WTM3
Gene Summary	May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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