Unc5c (NM_001293561) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B130051O18Rik; rcm; Unc5h3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229205 representing NM_001293561 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAAGGTCTGAGGGCGACAGCGGCCCGCTGCGGACTGGGACTAGGATACTTGCTGCAGATGCTTG TGTTACCTGCCCTGGCCCTGCTAAGCGCCAGTGGCACCGGCTCCGCCGCTCAAGATGATGAATTTTTTCA CGAACTCCCAGAAACCTTTCCATCTGACCCACCTGAGCCATTGCCACACTTCCTCATTGAGCCCGAGGAA GCTTACATTGTGAAGAACAAGCCTGTGAACCTGTATTGTAAAGCCAGCCCTGCCACCCAGATCTACTTCA AGTGCAACAGCGAGTGGGTTCATCAGAAGGACCACGTAGTAGACGAGAGAGTAGATGAAACCTCTGGTCT AATTGTGAGAGAAGTGAGCATTGAGATTTCACGCCAGCAGGTGGAGGAACTGTTTGGGCCTGAAGATTAC TGGTGCCAGTGTGTGGCCTGGAGCTCAGCAGGCACTACGAAGAGTCGGAAGGCATACGTGCGCATTGCGT ATCTGCGGAAGACATTCGAGCAGGAACCCTTGGGAAAGGAAGTGTCCTTGGAGCAGGAAGTCTTACTCCA GTGTCGGCCACCTGAAGGGATCCCAGTGGCTGAGGTAGAATGGCTAAAGAATGAAGACATAATTGATCCT GCTGAAGATCGGAACTTTTATATTACTATCGATCACAACCTGATCATCAAGCAAGCCCGACTCTCAGATA CAGCAAATTATACCTGTGTTGCCAAAAATATTGTTGCCAAGAGAAAAAGCACCACAGCCACTGTCATCGT GTATGTTAATGGTGGCTGGTCCACCTGGACAGAGTGGTCTGTGTGTAACAGCCGCTGTGGGCGAGGATAT CAGAAACGCACAAGAACCTGCACCAACCCAGCCCCACTCAATGGTGGGGCCTTCTGTGAGGGGCAGAGTG TGCAGAAAATAGCATGCACTACGTTATGTCCAGTGGATGGTAGGTGGACTTCATGGAGCAAATGGTCAAC CTGTGGGACTGAATGCACCCACTGGCGCAGGAGGGAGTGTACAGCACCAGCCCCCAAGAACGGGGGTAAG GACTGTGATGGCCTGGTCCTCCAATCCAAGAACTGCACTGATGGGCTGTGCATGCAGGGATTCATTTACC CCATTTCAACTGAGCACAGACCCCAGAATGAATATGGATTTTCTTCTGCTCCTGACTCAGATGATGTGGC TCTCTACGTGGGGATTGTGATCGCTGTAACAGTCTGTCTGGCGATCACTGTTGTGGTGGCCCTGTTTGTG TATCGGAAGAACCACCGTGACTTTGAGTCTGACATCATTGACTCCTCAGCACTCAATGGCGGCTTTCAGC CTGTGAACATCAAGGCTGCCAGACAAGATCTCCTGGCTGTCCCCCCTGACCTCACCTCAGCTGCAGCCAT GTACAGGGGACCTGTCTATGCTCTGCATGATGTCTCAGACAAAATCCCAATGACCAACTCTCCAATTCTG GACCCACTACCCAACTTGAAAATCAAAGTGTACAACAGCTCAGGTGCTGTCACTCCTCAGGATGACCTTG CCGAGTTCTCATCCAAACTGTCACCCCAGATGACCCAGTCCTTGCTAGAGAATGAGGCCCTTAACCTGAA GAACCAGAGCCTCGCAAGACAGACTGACCCATCCTGCACAGCATTTGGTACCTTCAACTCTCTTGGGGGT CACCTCATCATTCCTAATTCAGGAGTAAGCTTGCTGATTCCCGCTGGGGCCATTCCTCAGGGGAGAGTCT ATGAAATGTATGTGACTGTACACAGGAAAGAAAATATGAGGCCCCCCATGGAAGACTCTCAGACCCTACT TACCCCTGTGGTGAGCTGTGGGCCTCCTGGAGCTCTGCTGACCCGCCCTGTCATCCTCACTCTGCATCAC TGTGCAGACCCCAGCACCGAGGACTGGAAGATCCAGCTCAAAAACCAGGCAGTGCAGGGACAATGGGAGG ATGTTGTGGTGGTTGGGGAGGAGAACTTCACAACCCCCTGTTACATTCAGCTGGATGCAGAGGCTTGCCA TATCCTCACAGAGAACCTCAGTACCTATGCCCTGGTTGGGCAGTCCACCACCAAAGCAGCTGCCAAGCGT CTTAAACTGGCCATCTTTGGGCCCCTCTGCTGCTCTTCCCTGGAGTACAGCATTAGAGTCTACTGCCTGG ATGACACACAGGATGCCCTGAAGGAAGTTCTACAACTGGAGAGGCAAATGGGAGGACAGCTCCTAGAAGA ACCCAAGGCTCTTCATTTTAAAGGCAGCATCCACAACCTGCGCCTGTCTATTCATGACATCGCCCATTCC CTCTGGAAGAGCAAATTGCTGGCTAAGTATCAGGAAATTCCATTTTACCACATCTGGAGTGGCTCTCAAA GAAACCTCCACTGCACCTTCACTCTGGAAAGACTCAGCCTAAACACAGTGGAACTGGTTTGCAAACTCTG TGTGCGGCAGGTTGAAGGAGAAGGGCAGATCTTCCAGCTCAACTGTACTGTGTCAGAGGAACCTACTGGC ATCGACTTACCTCTCCTGGACCCTGCTAGTACCATCACCACTGTCACCGGACCAAGTGCTTTCAGCATTC CTCTCCCTATCCGGCAGAAGCTATGCAGCAGCCTGGATGCCCCTCAAACAAGAGGCCATGACTGGAGGAT GCTGGCCCATAAACTCAACCTGGACAGGTACTTGAATTACTTTGCCACCAAATCGAGCCCAACTGGCGTA ATCCTGGATCTTTGGGAAGCACAGAACTTCCCAGATGGAAACCTGAGCATGCTGGCAGCCGTCCTGGAAG AAATGGGAAGACATGAGACAGTGGTGTCCTTGGCAGCAGAAGGACAGTATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293561
Insert Size	2853 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293561.1, NP_001280490.1
RefSeq Size	9355 bp
RefSeq ORF	2853 bp
Locus ID	22253
Gene Summary	Receptor for netrin required for axon guidance (PubMed:22685302, PubMed:10399920). Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding (PubMed:10399920, PubMed:22685302). NTN1/Netrin-1 binding might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Axon repulsion in growth cones may also be caused by its association with DCC that may trigger signaling for repulsion (PubMed:10399920). Might also collaborate with DSCAM in NTN1-mediated axon repulsion independently of DCC (PubMed:22685302). Also involved in corticospinal tract axon guidance independently of DCC (PubMed:9126743, PubMed:9389662, PubMed:12451134). Involved in dorsal root ganglion axon projection towards the spinal cord (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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