Ap1b1 (NM_001243044) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Adtb1; b2b1660Clo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229202 representing NM_001243044 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCAAAATACTTCACCACAACCAAGAAAGGGGAGATCTTTGAGCTGAAGGCAGAGCTTAACA GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCAATGACTGTGGGCAAAGATGT CAGTGCTCTCTTCCCTGATGTGGTGAACTGCATGCAGACAGACAACCTGGAGCTAAAGAAGCTGGTGTAC CTGTACTTGATGAACTATGCCAAGAGTCAGCCTGACATGGCCATTATGGCTGTCAACACCTTTGTGAAGG ACTGTGAGGACCCCAATCCTCTCATCCGGGCCCTGGCTGTGAGGACTATGGGCTGCATCCGAGTTGACAA GATTACAGAGTACTTGTGTGAACCACTCCGGAAGTGCCTGAAGGATGAAGACCCATATGTGCGCAAGACA GCGGCTGTGTGTGTGGCCAAGCTCCATGACATCAATGCCCAGCTGGTAGAAGACCAGGGCTTCCTGGACA CGCTTAAAGACCTCATCTCAGACTCTAACCCCATGGTGGTAGCGAATGCAGTGGCTGCCCTGTCAGAGAT CGCTGAGTCTCACCCCAGTAGCAACCTGCTTGACTTGAACCCACAGTCTATCAACAAGCTGCTGACAGCC CTTAATGAGTGCACTGAATGGGGCCAGATCTTCATTCTGGACTGTCTGGCCAACTACATGCCCAAGGATG ACCGTGAGGCCCAGAGCATCTGTGAGCGGGTCACTCCTAGACTCTCCCATGCCAACTCTGCTGTGGTGCT GTCTGCGGTGAAGGTGCTGATGAAGTTCATGGAGATGCTGTCCAAGGACCTGGACTACTATGCCACACTG CTCAAGAAGCTGGCCCCTCCCCTGGTCACACTGCTGTCCGCAGAGCCAGAGCTGCAGTATGTGGCCCTGC GCAACATCAATCTCATTGTACAGAAAAGGCCAGAGATCTTGAAGCATGAAATGAAGGTTTTCTTTGTGAA GTACAATGACCCCATCTACGTGAAGCTGGAGAAACTGGACATTATGATCCGCCTGGCCTCCCAGGCCAAC ATTGCCCAGGTATTGGCAGAACTCAAAGAGTATGCTACAGAAGTAGACGTGGATTTCGTACGGAAGGCAG TGCGTGCCATTGGCCGCTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTGAGCACATTGCTTGA TCTCATCCAGACCAAGGTCAACTATGTGGTTCAGGAAGCCATTGTGGTCATTAAGGACATCTTCCGCAAG TACCCCAACAAGTATGAGAGTGTGATAGCTACACTGTGTGAGAATCTGGACTCTCTGGATGAGCCTGAGG CCCGAGCCGCTATGATCTGGATAGTGGGCGAGTATGCCGAGCGGATTGACAATGCAGACGAGCTGCTCGA GAGCTTCCTGGAAGGTTTCCATGATGAGAGCACTCAGGTCCAGCTACAGCTACTGACAGCCATTGTGAAA CTCTTCCTGAAGAAGCCAACAGAGACTCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT CAGACAATCCAGACCTTCGGGACCGTGGCTACATCTACTGGCGCTTGCTCTCCACGGACCCTGTAGCTGC TAAAGAGGTGGTACTGGCTGAGAAGCCCCTCATCTCAGAGGAGACAGACCTTATTGAGCCTACACTTCTG GATGAACTCATCTGCTACATTGGCACGCTGGCCTCTGTCTACCACAAGCCTCCCAACGCCTTTGTGGAGG GTGGCCGGGGAGTGGTACATAAGAGCCTGCCACCCCGCACTGCCTCGAGTGAGAGCACAGAGAGCCCTGA GACAGCTCCTGCTGGAGCACCTGCTGGTGATCAGCCTGATGTCATTCCTGCCCAGGGTGACCTGTTAGGT GACCTCCTCAACCTGGACCTTGGGCCCCCAGTGAGTGGCCCACCCTTAGCTGCCTCCTCCGTGCAAATGG GAGCTGTGGATCTTCTTGGTGGTGGCCTCGACAGCCTGATTGGAGATAGCAACTTTGGGGCACCTTCAGC ATCAGTGGCCGCAGCACCAGCACCAGCCAGACTTGGAGCACCCATCAGCAGTGGCTTAAGTGACCTCTTT GACCTGACCAGTGGTGTGGGCACCCTTTCAGGATCCTATGTAGCTCCCAAAGCAGTCTGGCTCCCTGCCA TGAAGGCCAAGGGACTGGAGATCTCTGGCACCTTCACCCGGCAGGCTGGCTCCATCTCTATGGACCTGCA GCTGACTAACAAGGCCCTGCAGGTCATGACAGATTTTGCTATCCAGTTCAATCGTAACAGCTTTGGCTTG GCCCCTGCTGCACCTCTCCAGGTGCACGTACCACTCAGCCCCAACCAGACCGTGGAGATCTCCCTGCCTC TTAACACAGTGGGCTCAGTCTTGAAGATGGAGCCTCTAAACAATCTTCAGGTGGCTGTGAAGAACAACAT TGACGTCTTCTACTTCAGCACTTTGTACCCACTGCATGTCCTCTTCGTGGAGGATGGGAAGATGGACCGG CAGATGTTCCTGGCCACATGGAAGGACATTGCCAACGAGAACGAAGCCCAGTTCCAGATCAGAGACTGCC CGCTTAACACAGAGGCTGCGAGCAACAAGCTACAGAGCAGTAACATTTTCACTGTCGCCAAGAGGAACGT GGAGGGCCAGGATATGCTCTACCAATCTCTGAAGTTGACCAATGGCATCTGGGTGCTGGCGGAGCTGCGG ATCCAGCCAGGCAACCCCAGCTTCACGGACTTGGAGCTGTCCCTGAAGTGTCGAGCACCGGAGGTGTCCC AGCATGTATACCAGGCCTACGAGACTATCCTCAAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243044
Insert Size	2841 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243044.1, NP_001229973.1
RefSeq Size	4118 bp
RefSeq ORF	2841 bp
Locus ID	11764
Gene Summary	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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