Ddr1 (NM_001198831) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6030432F18; AI323681; Cak; CD167a; Nep; PTK3A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229163 representing NM_001198831 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACAGGGACCCTCTCATCTCTACTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA AGGGACATTTTGACCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCTGACAGCGA TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCTGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACTTGCAGGTGGACCTTCGTA GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG AAGCTATCGGTTGCGTTACTCCCGAGATGGCCGCCGCTGGATGGACTGGAAGGACCGCTGGGGACAGGAG GTGATTTCGGGTAACGAGGATCCCGGGGGAGTAGTGCTGAAGGACCTTGGGCCCCCCATGGTGGCCCGGC TGGTCCGCTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT CTGGCGGGATGGACTCCTGTCATATACAGCCCCCGTGGGGCAGACCATGCAGTTATCTGAGGTGATGGTA CATCTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTATGGCGGTCTGGGCCAGCTGG CAGATGGCGTGGTGGGCCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGTCTGGCCAGGCTATGACTA TGTGGGATGGAGCAATCAGAGCTTCCCCACGGGCTACGTGGAGATGGAGTTTGAGTTTGATCGGTTGAGG ACCTTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTACCAGGCGGGGTGG AATGCCGGTTTAAAAGGGGTCCCGCCATGGCCTGGGAAGGAGAGCCTGTCCGCCATGCTCTGGGAGGCAG CCTTGGAGACCCCAGAGCCCGGGCCATCTCAGTGCCCCTGGGTGGCCACGTGGGCCGCTTTCTGCAGTGC AGATTCCTCTTTGCAGGTCCTTGGTTACTCTTCAGTGAGATCTCTTTCATCTCAGATGTGGTGAACGACT CCTCTGACACCTTCCCACCAGCCCCCTGGTGGCCACCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGA GCTGGAGCCCCGGGGTCAACAGCCAGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGC CTGGTGGCCATCATCCTGCTGCTGCTTCTCATCATCGCGCTGATGCTCTGGAGGCTGCACTGGCGCCGGC TGCTCAGCAAGGCTGAGCGCCGCGTGTTGGAGGAGGAGCTGACGGTTCACCTTTCTGTCCCTGGGGACAC CATCCTCATCAACAACCGCCCAGGACCCCGAGAGCCACCCCCTTACCAGGAGCCCCGGCCTCGGGGGACT CCACCCCATTCTGCACCCTGCGTCCCCAACGGCTCTGCGTTGCTGCTCTCCAATCCGGCCTACCGCCTCC TTCTGGCCACTTACGCCCGTCCCCCTCGAGGCCCGGGCCCCCCCACACCCGCCTGGGCCAAACCCACCAA CACCCAGGCCTGCAGTGGGGACTATATGGAGCCCGAGAAGCCGGGTGCCCCGCTTCTACCCCCACCTCCC CAGAACAGCGTCCCCCATTATGCCGAAGCTGACATTGTCACCCTGCAGGGCGTCACTGGGGGCAACACCT ACGCTGTGCCTGCACTGCCCCCAGGGGCGGTTGGGGATGGGCCCCCCAGAGTGGATTTCCCTCGGTCACG GCTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAATTTGGGGAGGTACACCTGTGTGAAGTAGAGGACCCG CAAGATCTGGTCAGTAGTGACTTCCCTATCAGTGTGCACAAGGGACACCCCTTGCTGGTAGCAGTGAAGA TCCTCCGGCCAGATGCCACCAAAAATGCCAGGAATGATTTCCTGAAGGAGGTAAAGATCATGTCACGGCT GAAGGACCCAAACATCATCCGGCTCCTGGGTGTGTGTGTGCAGGATGACCCCCTCTGCATGATCACAGAC TACATGGAGAACGGCGATCTGAACCAGTTCCTCAGTGCCCGCCAGCTGGAGAACAAGGCCACTCAGGGGC TCTCTGGGGACACAGAGTCTGACCAGGGGCCCACAATCAGCTACCCTATGCTGTTACACGTGGGGGCCCA GATCGCCTCTGGCATGCGTTATCTCGCCACGCTGAACTTTGTGCATCGGGACCTGGCCACCCGGAACTGC TTGGTTGGGGAAAATTTCACCATCAAAATCGCCGACTTTGGCATGAGCCGGAATCTCTACGCTGGGGATT ATTACCGTGTCCAGGGCCGGGCGGTGCTGCCCATCAGGTGGATGGCTTGGGAGTGCATTCTCATGGGGAA GTTCACAACAGCCAGTGACGTTTGGGCCTTCGGAGTGACCCTGTGGGAGGTGCTGATGCTCTGCAGGTCC CAGCCCTTTGGGCAGCTTACAGATGAGCAGGTTATCGAGAATGCCGGCGAGTTCTTCAGGGACCAGGGCC GGCAGGTCTACTTGTCCAGGCCACCCGCCTGCCCACAGACCCTGTATGAGCTGATGCTCCGGTGTTGGAG CCGGGAGCCCGAGCAGCGGCCGCCCTTCGCCCAGCTTCATCGGTTCCTGGCGGATGATGCGCTCAACACG GTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198831
Insert Size	2736 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198831.1, NP_001185760.1
RefSeq Size	3639 bp
RefSeq ORF	2736 bp
Locus ID	12305
UniProt ID	Q03146
Gene Summary	Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing, but also tumor cell invasion. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Phosphorylates PTPN11 (By similarity). Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same protein (isoform 1).

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