Invs (NM_001281977) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	inv; Nphp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229161 representing NM_001281977 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTCTTACTTACTCGAAGAGCAAACTGGATGCAGAAGGATCTAGAAGAGATGACACCTTTGCACC TGAGCACTCGGCACAGGAGCCCCAAGTGTCTAGCTCTTCTGCTGAAGTTTATGGCCCCTGGGGAGGTGGA TACACAGGACAAAAACAAGGACGCTGCCCCGACAGAGTCTTTACTGAACTGGCAAGACTACGAGGGCCGC ACACCTCTGCACTTTGCTGTCGCTGATGGAAACCTAACGGTGGTGGATGTCTTGACCTCCTATGAAAGCT GCAACATAACGTCTTACGATAACTTATTTCGAACCCCACTTCACTGGGCAGCCTTACTAGGTCATGCACA GATTGTCCATCTCCTTCTAGAAAGAAACAAGTCTGGAACGATCCCTTCGGACAGCCAGGGAGCAACACCC TTGCACTATGCAGCTCAGAGTAACTTTGCTGAAACGGTCAAAGTGTTTTTGCAGCATCCTTCAGTGAAGG ATGATTCTGACCTGGAAGGAAGAACATCTTTCATGTGGGCAGCAGGAAAAGGCAATGACGACGTGCTCAG GACGATGCTGAGTCTAAAGTCAGACATCGACATTAACATGTCGGACAAGTACGGAGGCACAGCTTTACAC GCCGCTGCCCTTTCTGGCCACGTCAGCACCGTGAAGTTATTATTGGACAATGATGCTCAAGTGGATGCCA CTGACGTCATGAAACATACTCCACTGTTCCGAGCCTGTGAGATGGGACACAGAGATGTGATTCAGACGCT TATTAAAGGTGGAGCACGAGTAGATCTGGTTGACCAGGACGGACATTCGCTTCTACACTGGGCAGCGCTG GGGGGAAATGCTGACGTCTGCCAGATACTGATAGAAAATAAGATCAACCCAAACGTGCAGGATTATGCAG GAAGAACACCGCTTCAGTGTGCTGCGTACGGAGGGTACATCAACTGTATGGCTGTGCTCATGGAGAATAA TGCAGACCCCAACATCCAAGACAAAGAGGGACGAACAGCTTTGCACTGGTCCTGTAACAATGGCTACCTT GATGCCATTAAACTACTGCTAGATTTTGCTGCTTTCCCTAATCAGATGGAGAACAATGAGGAGAGGTACA CACCCCTTGATTATGCATTGCTTGGTGAGCGCCATGAGGTGATCCAGTTCATGCTGGAACATGGTGCCCT GTCCATCGCAGCTATACAAGACATCGCTGCCTTCAAAATCCAAGCAGTCTACAAAGGATACAAGGTCAGA AAAGCCTTCCGAGACCGGAAGAACCTCCTCATGAAGCACGAACAGCTGAGGAAAGACGCTGCAGCCAAAA AACGGGAAGAGGAAAACAAGAGGAAGGAAGCTGAACAGCAGAAAGGGCAGCTGGACACCGATCCTCCCAG ATCCCACTGCAGTTCATCTGCACCTGTCCTCCCCTGTCCACCCAGCCCCCAGAATGAGGCCAGCAAGCAG GACGCAACCCCTAGCAAACAGCCACCTGCTAGCCACACAGTCCAGAGCCCTGACCCAGAACACAGCAGGC CTCCAGGCAGATGTCCAGGCAGAGCCTCCCAAGGGGATTCTTCCATAGACCTTCAGGGAACGGCCTCCAG AAAGCCAAGTGAAACACCCATAGAGCACTGCAGAGGCCCCTCTGCCTGTGTGCACCCCAGATCCTGGGAA GGTGGCAACAGCTCCAAGAACCAGGGAACATCCTCTGTGGAGAAGCGCAGAGGTGAGACCAATGGCAAAC ACCGGAGGTGTGAGGAGGGGCCCAGCAGCGCCAGGCAGCCCTTGTGCACTGGGTCAGGCCGGCCTGCTGA GAAAGGAGAGGACTCCAGCCCTGCAGTTGCAAGTGCTTCCCAGCAGGACCACCCCAGGAAGCCCAATAAG AGACAGGACAGGGCAGCCAGGCCCAGAGGTGCTTCGCAGAAAAGACGCACCCATCAGCTCAGAGACAGGT GCTCCCCAGCTGGGTCCAGCCGGCCTGGCAGTGCCAAGGGGGAAGTTGCCTGTGCTGATCAGAGTTCTCT CCACCGTCACACACCAAGAAGCAAGGTGACCCAGGACAAGCTTATAGGAGGGGTCTCCTCAGGTTTGCCG CTCAGCACAGAAGCGTCGAGGTCAGGATGCAAGCAGCTTTATGAGGACATCTGTGCATCTCCAGAGACTG GCGTGGCTCATGGCCCCCCACCTGGACAGTGTATGAATATTCACCTTCTTCCTGTAGAGCAGCGCCTGCT GATAATCCAGAGGGAACGAAGCAGGAAAGAGCTGTTTCGACGGAAAAACAAGGCAGCGGCGGTCATCCAG CGAGCTTGGCGAAGCTACCAGCTCAGGAAGCACCTGTCTCGGCTTCTGCATTTGAAGCAGCTTGGAGCCA GAGAAGTGCTCAGATGTACCCAAGTGTGCACAGCCCTGCTCCTCCAGGTTTGGAGGAAAGAACTGGAACT CAAATTCCCAAAGTCCATCTCAGTAAGCAGGACATCGAAGAGTCCATCCAAAGGCTCGTCGGCCACAAAG TATGCCAGGCACTCAGTGCTCAGGCAGATCTACGGTTGTTCTCAAGAAGGGAAAGGACATCATCCCATCA AATCTTCTAAAGCCCCCGCTGTGCTTCATCTCAGCTCAGTGAACAGTTTGCAGTCTATACATCTTGACAA CAGTGGAAGATCAAAGAAGTTCTCTTACAATCTGCAACCATCCAGTCAATCAAAAAACAAGCCAAAGCTT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281977
Insert Size	2733 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281977.1, NP_001268906.1
RefSeq Size	5622 bp
RefSeq ORF	2733 bp
Locus ID	16348

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