Plk4 (NM_173169) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700028H20; AI385771; S; Sak; Stk1; Stk18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229135 representing NM_173169 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTGCATCGGGGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTCGGTAAAGGATCATTTG CTGGTGTCTACAGAGCTGAGTCCATACACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC CATGTACAAAGCTGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAGTTGAAACACCCC TCTGTCTTGGAGCTCTATAATTACTTTGAAGATAACAATTATGTCTACCTGGTATTGGAAATGTGCCACA ATGGAGAAATGAACAGATATCTGAAGAACAGAATGAAGCCTTTCTCAGAAAGGGAAGCTAGGCACTTCAT GCACCAGATTATCACAGGAATGTTATATCTTCATTCTCATGGCATATTGCACCGGGACCTCACACTCTCT AACATCTTACTTACGCGGAATATGAACATAAAAATTGCTGACTTTGGACTAGCAACGCAGTTGAATATGC CACATGAAAAGCACTATACACTCTGTGGGACTCCTAATTATATTTCACCAGAAATTGCAACTCGAAGTGC ACATGGACTTGAATCTGATATTTGGTCATTGGGCTGTATGTTTTATACGTTACTTATTGGAAGACCACCT TTTGACACTGACACAGTCAAGAACACATTGAACAAAGTAGTCCTGGCAGATTATGAAATGCCAGCCTTTT TGTCACGAGAGGCCCAGGACCTTATCCACCAGTTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCTGTC TTCTGTGTTGGACCATCCTTTCATGTCACGAAATCCTTCACCAAAGAGTAAAGACGTAGGGACTGTAGAG GACTCAATGGATAGTGGGCATGCTACACTTTCCACAACAATTACAGCCTCTTCTGGTACCAGTTTGAGTG GCAGCCTACTTGACAGAAGACTTTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAAAAA TAAAAATTCAAGTGACTTTTCTTCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAACAA GAAGCTAATAGTAGGGGACGGGGGAGAGTGATTGAAGATGCAGAAGAGAGGCCGCATTCTCGATACCTGC GCAGAGCTCATTCCTCTGATAGAGCCAGCCCCTCTAATCAGTCTCGAGCAAAAACATACTCAGTAGAACG TTGTCACTCAGTAGAAATGCTTTCAAAGCCTAGAAGATCACTGGATGAAAATCAACACAGTTCCAATCAT CATTGTCTAGGAAAAACTCCTTTTCCATTTGCAGACCAGACACCTCAGATGGAAATGGTACAGCAGTGGT TTGGGAATCTGCAAATGAATGCTCATTTAGGAGAAACTAATGAGCACCACACCGTTAGCCCAAACAGAGA TTTCCAGGACTATCCAGATTTGCAGGACACGTTACGAAACGCTTGGACTGACACGAGAGCCAGCAAGAAT GCTGATACTTCTGCCAATGTTCATGCTGTAAAGCAGCTGAGTGCCATGAAATACATGAGTGCACATCACC ATAAGCCTGAGGTCATGCCACAGGAGCCGGGCCTACATCCTCATTCTGAACAAAGCAAGAATAGAAGTAT GGAGTCGACACTGGGTTACCAGAAACCTACCTTAAGAAGTATTACATCTCCTCTGATTGCTCACAGATTA AAGCCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATCCTTGATTCAGAGGAGATCACTGTTTATT ACCCGAACGATGGAAGAGGCTTTCCTCTTGCTGACAGACCTCCCTTGCCTACTGACAACATCAGTAGGTA CAGCTTTGACAATCTACCAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGATTCATTCAGCTAGTA AGATCTAAAACTCCCAAAATCACTTATTTTACAAGATATGCTAAATGTATTTTGATGGAAAATTCTCCTG GTGCTGATTTCGAAGTTTGGTTTTATGATGGAGCCAAAATACATAAAACTGAAAATTTAATTCACATAAT TGAGAAAACAGGGATATCTTATAATTTAAAAAATGAAAATGAAGTTACCAGCCTGAAAGAGGAAGTAAAA GTATATATGGACCATGCTAATGAGGGTCACCGTATTTGCTTGTCACTGGAATCTGTAATCTCTGAGGAGG AAAAGAGAAGCAGGGGTTCTTCATTCTTCCCTATAATCGTAGGAAGAAAACCTGGTAATACTAGTTCACC TAAAGCCTTATCAGCTCCTCCTGTGGACCCAAGCTGCTGTAAGGGAGAGCAGGCGTCAGCAAGCAGACTG AGCGTGAATAGTGCCGCTTTCCCCACACAGTCCCCAGGACTCAGTCCTTCCACTGTGACAGTTGAAGGAC TTGGCCACACAGCGACTGCCACAGGAACAGGCGTCTCTTCAAGTCTTCCTAAATCTGCACAGCTTTTGAA ATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGCTAACTAGCGGAGCTGTGTGGGTTCAGTTTAAT GATGGGTCACAGTTGGTTGTCCAGGCAGGAGTATCTTCCATCAGTTACACATCACCAGATGGTCAGACAA CTAGGTATGGAGAAAATGAAAAATTACCTGAATACATCAAACAGAAATTACAGTGTCTTTCTTCCATCCT TCTGATGTTTTCTAATCCAACTCCTAATTTTCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_173169
Insert Size	2697 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_173169.2, NP_775261.2
RefSeq Size	3486 bp
RefSeq ORF	2697 bp
Locus ID	20873
UniProt ID	Q64702
Gene Summary	This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2, to centrosomes during G2/M, and to the cleavage furrow during cytokinesis. It is required for progression through mitosis, cell survival, and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The transcription start site was identified in PMID:10607900.

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